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Effects of Repeated Applications of Chlorimuron-Ethyl on the Soil Microbial Biomass, Activity and Microbial Community in the Greenhouse

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Abstract

The impacts of repeated chlorimuron-ethyl applications on soil microbial community structure and function were studied under greenhouse conditions. Chlorimuron-ethyl was applied to soil samples at three different doses [1-,10-,100-fold of recommended field rate (T1, T10, T100)] for 3 years. The half-lives of chlorimuron-ethyl were 37.1–54.6 days. The soil microbial biomass (microbial biomass carbon and total phospholipid fatty acid), the microbial activity (basal respiration and average well color development), the ratio of Gram-negative/Gram-positive bacteria and Shannon index were stimulated by chlorimuron-ethyl during the initial period. Except for T100, the other treatments recovered to the untreated level. The ratio of fungi/bacteria decreased during the initial period and then recovered in the end. Principal component analysis of phospholipid fatty acid showed that chlorimuron-ethyl altered the microbial community structure. Except got T100, T1 and T10 were not different from the control at the end of experiment. These results suggested a dosage effect of chlorimuron-ethyl on the living microbial biomass and the microbial community.

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Acknowledgments

This work was supported financially by the Nature Science Foundation of China (NSFC, 31000863, 31171879) and Public service sector R&D Project (201203098).

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Authors and Affiliations

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Jun Xu, Ying Zhang, Fengshou Dong, Xingang Liu, Xiaohu Wu & Yongquan Zheng

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  1. Jun Xu
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  2. Ying Zhang
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  3. Fengshou Dong
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  4. Xingang Liu
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  5. Xiaohu Wu
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  6. Yongquan Zheng
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Corresponding author

Correspondence to Yongquan Zheng.

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Jun Xu and Ying Zhang have contributed equally to this paper.

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Xu, J., Zhang, Y., Dong, F. et al. Effects of Repeated Applications of Chlorimuron-Ethyl on the Soil Microbial Biomass, Activity and Microbial Community in the Greenhouse. Bull Environ Contam Toxicol 92, 175–182 (2014). https://doi.org/10.1007/s00128-013-1156-7

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  • DOI: https://doi.org/10.1007/s00128-013-1156-7

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