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Nickel Detoxification and Plant Growth Promotion by Multi Metal Resistant Plant Growth Promoting Rhizobium Species RL9

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Abstract

Pollution of the biosphere by heavy metals is a global threat that has accelerated dramatically since the beginning of industrial revolution. The aim of the study is to check the resistance of RL9 towards the metals and to observe the effect of Rhizobium species on growth, pigment content, protein and nickel uptake by lentil in the presence and absence of nickel. The multi metal tolerant and plant growth promoting Rhizobium strain RL9 was isolated from the nodules of lentil. The strain not only tolerated nickel but was also tolerant o cadmium, chromium, nickel, lead, zinc and copper. The strain tolerated nickel 500 μg/mL, cadmium 300 μg/mL, chromium 400 μg/mL, lead 1,400 μg/mL, zinc 1,000 μg/mL and copper 300 μg/mL, produced good amount of indole acetic acid and was also positive for siderophore, hydrogen cyanide and ammonia. The strain RL9 was further assessed with increasing concentrations of nickel when lentil was used as a test crop. The strain RL9 significantly increased growth, nodulation, chlorophyll, leghaemoglobin, nitrogen content, seed protein and seed yield compared to plants grown in the absence of bioinoculant but amended with nickel The strain RL9 decreased uptake of nickel in lentil compared to plants grown in the absence of bio-inoculant. Due to these intrinsic abilities strain RL9 could be utilized for growth promotion as well as for the remediation of nickel in nickel contaminated soil.

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Authors and Affiliations

  1. Department of Biological Sciences, College of Natural and Applied Sciences, Crescent University, Abeokuta, Ogun State, Nigeria

    Parvaze Ahmad Wani

  2. Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, UP, India

    Mohammad Saghir Khan

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  1. Parvaze Ahmad Wani
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  2. Mohammad Saghir Khan
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Correspondence to Parvaze Ahmad Wani.

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Wani, P.A., Khan, M.S. Nickel Detoxification and Plant Growth Promotion by Multi Metal Resistant Plant Growth Promoting Rhizobium Species RL9. Bull Environ Contam Toxicol 91, 117–124 (2013). https://doi.org/10.1007/s00128-013-1002-y

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  • DOI: https://doi.org/10.1007/s00128-013-1002-y

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