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Isolation and Functional Characterization of Siderophore-Producing Lead- and Cadmium-Resistant Pseudomonas putida KNP9

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Abstract

Heavy metals, being phytotoxic, cause growth inhibition and even plant death. Siderophore-producing bacterial strain KNP9 is growth promoting and has been isolated from Panki Power Plant, Kanpur, India. It simulated significant (p > 5%) root and shoot growth of mung bean to the extent of 16.48% and 28.80%, respectively in the presence of CdCl2 (110 μM). However, the increase in root and shoot growth was 20% and 19.5%, respectively, in the presence of (CH3COO)2Pb (660 μM). Moreover, concentration of accumulated lead and cadmium in root and shoot was also reduced in the presence of this isolate ranging from 37.5 to 93.19%. A moderate reduction in chlorophyll content (39.14%) in the presence of 110 μM CdCl2 was rescued by bioinoculant KNP9. However, the 19.58% decrease in chlorophyll content in the case of lead acetate remained unchanged even in the presence of KNP9. Nevertheless, 16S ribosomal DNA (rDNA) sequencing identified KNP9 as a strain of Pseudomonas putida.

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Acknowledgments

This work is supported by Department of Biotechnology grant to R.G. The senior author (M.T.) acknowledged the CSIR for financial assistance in terms of J.R.F. We also thank Dr. Mahesh Kumar, Epidemology and Preventive Medicine C.Vsc., for the heavy metal analysis and Dr. B. Saini for carefully reading the manuscript.

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Authors and Affiliations

  1. Department of Microbiology, CBSH, G.B. Pant University of Agriculture & Technology, Pantnagar-263145, India

    Manishi Tripathi & Reeta Goel

  2. National Centre for Cell Science, Pune University Campus, Ganeshkhind, Pune-411007, India

    Hitendra P. Munot & Yogesh Shouche

  3. Laboratoire de Microbiologie et de Genetique, CNRS UPRES-A7010, Université Louis-Pasteur, 67000, Strasbourg, France

    Jean Marie Meyer

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  1. Manishi Tripathi
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  2. Hitendra P. Munot
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  4. Jean Marie Meyer
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  5. Reeta Goel
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Correspondence to Reeta Goel.

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Tripathi, M., Munot, H.P., Shouche, Y. et al. Isolation and Functional Characterization of Siderophore-Producing Lead- and Cadmium-Resistant Pseudomonas putida KNP9. Curr Microbiol 50, 233–237 (2005). https://doi.org/10.1007/s00284-004-4459-4

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