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Genetic dissection of the maize (Zea mays L.) MAMP response

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Loci associated with variation in maize responses to two microbe-associated molecular patterns (MAMPs) were identified. MAMP responses were correlated. No relationship between MAMP responses and quantitative disease resistance was identified.

Abstract

Microbe-associated molecular patterns (MAMPs) are highly conserved molecules commonly found in microbes which can be recognized by plant pattern recognition receptors. Recognition triggers a suite of responses including production of reactive oxygen species (ROS) and nitric oxide (NO) and expression changes of defense-related genes. In this study, we used two well-studied MAMPs (flg22 and chitooctaose) to challenge different maize lines to determine whether there was variation in the level of responses to these MAMPs, to dissect the genetic basis underlying that variation and to understand the relationship between MAMP response and quantitative disease resistance (QDR). Naturally occurring quantitative variation in ROS, NO production, and defense genes expression levels triggered by MAMPs was observed. A major quantitative traits locus (QTL) associated with variation in the ROS production response to both flg22 and chitooctaose was identified on chromosome 2 in a recombinant inbred line (RIL) population derived from the maize inbred lines B73 and CML228. Minor QTL associated with variation in the flg22 ROS response was identified on chromosomes 1 and 4. Comparison of these results with data previously obtained for variation in QDR and the defense response in the same RIL population did not provide any evidence for a common genetic basis controlling variation in these traits.

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Acknowledgements

This work was funded in part by DOE grant #DE-SC0014116, “Physiological and Molecular-Genetic Characterization of Basal Resistance in Sorghum”; XZ’s fellowship was funded by the China Scholarship Council Fellowship; we would like to thank Carole Saravitz, Joe Chiera, and the staff in NCSU Phytotron for their management of our plant materials, Jim Holland (USDA-ARS) and Sherry Flint-Garcia (USDA-ARS) for seed of the CML228 × B73 mapping population; we also would like to thank Jonathan Kressin (NCSU Department of Horticultural Science), Wall Crumpler, and John Godwin for access to and help with using the plate-reader, and Jenny Kimball, Yangrong Cao, Qin Yang, Yijian He, Guanfeng Wang, and Shannon Sermons for help, advice, and access to various materials.

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  1. Oswaldo Valdés-López

    Present address: Laboratorio de Genomica Funcional de Leguminosas, FES Iztacala, UNAM, Tlalnepantla, 54090, Mexico

Authors and Affiliations

  1. Maize Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, China

    Xinye Zhang

  2. Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA

    Xinye Zhang & Peter Balint-Kurti

  3. Division of Plant Science and Biochemistry, University of Missouri, Columbia, MO, 65211, USA

    Oswaldo Valdés-López & Gary Stacey

  4. Statistics Department, North Carolina State University, Raleigh, NC, 27695, USA

    Consuelo Arellano

  5. U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS) Plant Science Research Unit, Raleigh, NC, USA

    Peter Balint-Kurti

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Contributions

PBK, GS, XZ and OVL planned the research, XZ and OVL performed the research and analyzed the data, CA performed statistical analyses, XZ and PBK wrote the manuscript, and GA, OVL, and CA edited the manuscript.

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Correspondence to Peter Balint-Kurti.

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This work was funded in part by DOE Grant #DE-SC0014116, “Physiological and Molecular-Genetic Characterization of Basal Resistance in Sorghum”; XZ’s fellowship was funded by the China Scholarship Council Fellowship.

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The authors declare that they have no conflict of interest.

This article does not contain any studies with human participants or animals performed by any of the authors.

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Communicated by Jianbing Yan.

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Zhang, X., Valdés-López, O., Arellano, C. et al. Genetic dissection of the maize (Zea mays L.) MAMP response. Theor Appl Genet 130, 1155–1168 (2017). https://doi.org/10.1007/s00122-017-2876-6

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