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Evidence of intralocus recombination at the Glu-3 loci in bread wheat (Triticum aestivum L.)

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Recombination at the Glu-3 loci was identified, and strong genetic linkage was observed only between the amplicons representing i-type and s-type genes located, respectively, at the Glu-A3 and Glu-B3 loci.

Abstract

The low-molecular weight glutenin subunits (LMW-GSs) are one of the major components of wheat seed storage proteins and play a critical role in the determination of wheat end-use quality. The genes encoding this class of proteins are located at the orthologous Glu-3 loci (Glu-A3, Glu-B3, and Glu-D3). Due to the complexity of these chromosomal regions and the high sequence similarity between different LMW-GS genes, their organization and recombination characteristics are still incompletely understood. This study examined intralocus recombination at the Glu-3 loci in two recombinant inbred line (RIL) and one doubled haploid (DH) population, all segregating for the Glu-A3, Glu-B3, and Glu-D3 loci. The analysis was conducted using a gene marker system that consists of the amplification of the complete set of the LMW-GS genes and their visualization by capillary electrophoresis. Recombinant marker haplotypes were detected in all three populations with different recombination rates depending on the locus and the population. No recombination was observed between the amplicons representing i-type and s-type LMW-GS genes located, respectively, at the Glu-A3 and Glu-B3 loci, indicating tight linkage between these genes. Results of this study contribute to better understanding the genetic linkage and recombination between different LMW-GS genes, the structure of the Glu-3 loci, and the development of more specific molecular markers that better represent the genetic diversity of these loci. In this way, a more precise analysis of the contribution of various LMW-GSs to end-use quality of wheat may be achieved.

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Acknowledgements

The authors thank Dr. Richard Frohberg for providing the Butte 86 × ND2603 population and Dr. Arron H. Carter for providing the Brundage × Coda population. Stacey Sykes and Shawna Vogl assisted in the preparation and submission of the manuscript. This research was supported in part by NIFA AFRI Grant No. 2013-67013-21226.

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Authors and Affiliations

  1. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, USA

    Maria I. Ibba

  2. USDA-ARS Western Wheat Quality Laboratory, Pullman, WA, USA

    Alecia M. Kiszonas & Craig F. Morris

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  1. Maria I. Ibba
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  2. Alecia M. Kiszonas
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  3. Craig F. Morris
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Correspondence to Craig F. Morris.

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Mention of trademark or proprietary products does not constitute a guarantee or warranty of a product by the US Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. This article is in the public domain and not copyrightable.

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Ibba, M.I., Kiszonas, A.M. & Morris, C.F. Evidence of intralocus recombination at the Glu-3 loci in bread wheat (Triticum aestivum L.). Theor Appl Genet 130, 891–902 (2017). https://doi.org/10.1007/s00122-017-2858-8

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