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Transfer of sclerotinia resistance from wild relative of Brassica oleracea into Brassica napus using a hexaploidy step

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Sclerotinia resistance was transferred into rapeseed from a wild relative of Brassica oleracea ( B. incana ) using hexaploids derived from crosses between B. incana and rapeseed as a bridge.

Abstract

A high level of resistance against Sclerotinia sclerotiorum has been documented in wild Brassica oleracea, but not in cultivated rapeseed (Brassica napus). To transfer sclerotinia resistance from a wild relative into rapeseed, a strategy was proposed using hexaploids (AACCCC) derived from crosses between the wild B. oleracea-related B. incana genotype ‘C01’ and the Chinese rapeseed variety ‘Zhongshuang 9’ as a bridge. Progenies (BC1F1) generated by backcrossing the hexaploid to ‘Zhongshuang 9’ could be generated with a high crossability (average 18.3 seeds per pod). Seventy-three individuals in BC1F1 were firstly screened for resistance with five molecular markers linked to the major resistance QTL on chromosome C09 in ‘C01’, and 11 individuals harboring resistance loci were selected to develop vegetative clones. Of these, five exhibited significantly higher resistance than ‘Zhongshuang 9’ and the most resistant individual was chosen to develop the BC1F2 progeny. Finally, five individual genotypes with nearly twofold higher resistance than ‘Zhongshuang 9’ were found among 100 BC1F2 individuals by using marker-assisted selection and resistance evaluation. Hereof, one rapeseed-type individual with 38 chromosomes and good self-fertility (15.0 ± 3.56 seeds/pod) was identified. Our results indicate that the proposed strategy is effective for transferring sclerotinia resistance from a wild relative of B. oleracea into rapeseed.

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Acknowledgments

We thank Elke Diederichsen (Free University of Berlin, Germany) for providing the S. sclerotiorum isolate, and Peter M. Gresshoff (the University of Queensland, Australia) for correcting the manuscript. This study was financially supported by “Forschungsund Entwicklungsfonds Raps’’ in Germany, the 973 Program (2015CB150201), the Key Projects in the National Science and Technology (2014BAD01B07), NSFC (31401861, 31401411, 31171585) and the Fundamental Research Funds for the Central Universities (XDJK2013A013, XDJK2014A015, SWU113106 and XDJK2014B036) in China.

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The authors declare that they have no conflict of interest.

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Correspondence to Wei Qian or Wolfgang Friedt.

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Communicated by Richard G.F. Visser.

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Mei, J., Liu, Y., Wei, D. et al. Transfer of sclerotinia resistance from wild relative of Brassica oleracea into Brassica napus using a hexaploidy step. Theor Appl Genet 128, 639–644 (2015). https://doi.org/10.1007/s00122-015-2459-3

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  • DOI: https://doi.org/10.1007/s00122-015-2459-3

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