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Improvement of Sclerotinia sclerotiorum resistance in Brassica napus by using B. oleracea

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Abstract

Sclerotinia stem rot is one of the most serious diseases in rapeseed (Brassica napus) due to the lack of resistance sources. A high level of resistance was reported in Brassica oleracea cytodeme, one of parental species of rapeseed. In this study, a panel of 55 resynthesized lines of B. napus (RS lines) derived from seven wild and two cultivated types of B. oleracea was evaluated for Sclerotinia resistance over 2 years. Relative to ‘Zhongyou 821’, a cultivar of B. napus with partial resistance against S. sclerotiorum, RS lines exhibited stronger stem resistance. Although the resistant level of RS lines was lower than that of corresponding parental B. oleracea, a moderate correlation between resistance of RS line and corresponding parental B. oleracea type was found both for leaf (r = 0.74, P = 0.02) and stem (r = 0.69, P = 0.04). Our data suggests that the RS lines are important resources to improve Sclerotinia resistance of current rapeseed. A breeding strategy is discussed to enhance the Sclerotinia resistance of rapeseed by using B. oleracea.

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Acknowledgments

We sincerely thank Dr. Martin Frauen for the discussion on this study. This work was supported financially by grants from Special Fund for Agroscientific Research in the Public Interest (201103016), NSFC (31171585), CSTC 201180001, National Key Technology R & D Program (2010BAD01B02), DAAD (A/10/00321) and 111 Project (B12006).

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Authors and Affiliations

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China

    Yijuan Ding, Jiaqin Mei, Qinfei Li, Yao Liu, Huafang Wan, Lei Wang & Wei Qian

  2. Department of Crop Sciences, Georg-August-Universität Göttingen, Von-Siebold-Str. 6, 37075, Göttingen, Germany

    Heiko C. Becker

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  1. Yijuan Ding
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  2. Jiaqin Mei
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  3. Qinfei Li
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  4. Yao Liu
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  5. Huafang Wan
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  6. Lei Wang
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  7. Heiko C. Becker
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  8. Wei Qian
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Correspondence to Wei Qian.

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10722_2013_9978_MOESM1_ESM.xls

Parentage origin and relative Sclerotinia susceptibility to rapeseed ‘Zhongyou 821’ of 55 resynthesized lines used in the study. The types of B. oleracea cytodeme were identified in the previous study (Mei et al. 2011b) (XLS 33 kb)

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Ding, Y., Mei, J., Li, Q. et al. Improvement of Sclerotinia sclerotiorum resistance in Brassica napus by using B. oleracea . Genet Resour Crop Evol 60, 1615–1619 (2013). https://doi.org/10.1007/s10722-013-9978-z

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