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Wild peas vary in their cross-compatibility with cultivated pea (Pisum sativum subsp. sativum L.) depending on alleles of a nuclear–cytoplasmic incompatibility locus

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Abstract

Key message

Divergent wild and endemic peas differ in hybrid sterility in reciprocal crosses with cultivated pea depending on alleles of a nuclear ‘speciation gene’ involved in nuclear–cytoplasmic compatibility.

Background

In hybrids between cultivated and wild peas, nuclear–cytoplasmic conflict frequently occurs. One of the nuclear genes involved, Scs1, was earlier mapped on Linkage Group III.

Results

In reciprocal crosses of seven divergent pea accessions with cultivated P. sativum, some alleles of Scs1 manifested incompatibility with an alien cytoplasm as a decrease in pollen fertility to about 50 % in the heterozygotes and lack of some genotypic classes among F2 segregants. Earlier, we defined monophyletic evolutionary lineages A, B, C and D of pea according to allelic state of three markers, from nuclear, plastid and mitochondrial genomes. All tested representatives of wild peas from the lineages A and C exhibited incompatibility due to Scs1 deleterious effects in crosses with testerlines of P. sativum subsp. sativum (the common cultivated pea) at least in one direction. A wild pea from the lineage B and a cultivated pea from the lineage D were compatible with the testerline in both directions. The tested accession of cultivated P. abyssinicum (lineage A) was partially compatible in both directions. The Scs1 alleles of some pea accessions even originating from the same geographic area were remarkably different in their compatibility with cultivated Pisum sativum cytoplasm.

Conclusion

Variability of a gene involved in reproductive isolation is of important evolutionary role and nominate Scs1 as a speciation gene.

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Acknowledgments

This work has been supported by Russian Foundation for Fundamental Research, Grant number 13-04-00516A and the project VI.53.1.3. Pollen counts were made with the use of the Centre of Microscopy of Biological Objects of ICG SB RAS.

Ethical statement

The experiments comply with the current laws of the country in which they were performed.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institute of Cytology and Genetics of Siberian Division of Russian Academy of Sciences, Acad. Lavrentyev ave. 10, Novosibirsk, 630090, Russia

    V. S. Bogdanova, O. E. Kosterin & A. K. Yadrikhinskiy

  2. Novosibirsk State University, Pirogova str. 2, Novosibirsk, 630090, Russia

    O. E. Kosterin

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  1. V. S. Bogdanova
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  2. O. E. Kosterin
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  3. A. K. Yadrikhinskiy
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Correspondence to O. E. Kosterin.

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Communicated by Y. Xue.

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Bogdanova, V.S., Kosterin, O.E. & Yadrikhinskiy, A.K. Wild peas vary in their cross-compatibility with cultivated pea (Pisum sativum subsp. sativum L.) depending on alleles of a nuclear–cytoplasmic incompatibility locus. Theor Appl Genet 127, 1163–1172 (2014). https://doi.org/10.1007/s00122-014-2288-9

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  • DOI: https://doi.org/10.1007/s00122-014-2288-9

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