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Mapping of quantitative trait loci for a new source of resistance to bruchids in the wild species Vigna nepalensis Tateishi & Maxted (Vigna subgenus Ceratotropis)

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Abstract

Azuki bean breeders have long been interested in producing azuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] varieties with bruchid resistance. A new bruchid (Callosobruchus spp.) resistance source was found in V. nepalensis Tateishi & Maxted, a species that is cross compatible with azuki bean. Quantitative trait loci (QTLs) analysis for resistance to C. chinensis (L.) and C. maculatus (F.) was conducted using F2 (V. nepalensis × V. angularis) and BC1F1 [(V. nepalensis × V. angularis) × V. angularis] populations derived from crosses between the bruchid resistant species V. nepalensis and bruchid susceptible species V. angularis. Resistance was measured using two traits, percentage of seeds damaged by bruchids and the time taken for adult bruchids to emerge from seeds. Based on the results from both populations seven QTLs were detected for bruchid resistance; five QTLs for resistance to C. chinensis and two QTLs for resistance to C. maculatus. The different locations found for some resistance QTL to the two bruchid species suggests different resistance mechanisms. QTLs on linkage group (LG) 1 and LG2 for bruchid resistance to C. chinensis co-localized with seed size QTLs suggesting that incremental increase in seed size accompanied susceptibility to C. chinensis. Based on linked markers the QTL on these two linkage groups appear to be the same as previously reported in other Asian Vigna. However, several other QTLs were newly detected including one on LG4 that appears unrelated to seed size. Transfer of these new sources of bruchid resistance from V. nepalensis to azuki bean will be aided by the progress being made in azuki genome mapping.

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Acknowledgments

This study was conducted when the first author (PS) was a recipient of a Royal Golden Jubilee Ph.D. Scholarship awarded from the Thailand Research Fund.

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Authors and Affiliations

  1. Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, 73140, Thailand

    Prakit Somta & Peerasak Srinives

  2. Genebank, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602, Japan

    Akito Kaga, Norihiko Tomooka, Takehisa Isemura & Duncan A. Vaughan

Authors
  1. Prakit Somta
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  2. Akito Kaga
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  3. Norihiko Tomooka
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  4. Takehisa Isemura
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  5. Duncan A. Vaughan
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  6. Peerasak Srinives
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Corresponding author

Correspondence to Duncan A. Vaughan.

Additional information

Communicated by H. T. Nguyen.

Electronic supplementary material

Below is the link to the electronic supplementary material.

S1.

QTLs detected in the self pollinated (F2) and backcrossed (BC1F1) populations by interval mapping and/or multiple interval mapping. (DOC 37 kb)

S2.

QTL locations determined by interval mapping and/or multiple interval mapping of C. chinensis resistance, C. maculatus resistance, and seed size on comparative linkage map between F2 (V. nepalensis x V. angularis) and BC1F1 [(V. nepalensis x V. angularis) x V. angularis] maps. Linkage groups of F2 and BC1F1 maps are aligned on the left and right sides, respectively. Lines connect common markers between linkage groups. The QTL locations confirmed by the both mapping populations are indicated by arrow. Horizontal bold bars are the positions that revealed statistically significant association with traits. Vertical gray lines from them represents their standard deviations obtained by bootstrap. (TIFF 27591 kb)

S3.

Evaluation of bruchid resistance to C. chinensis and C. maculatus using seeds from different cross combinations between V. nepalensis and V. angularis (DOC 42 kb)

S4.

Evaluation of bruchid resistance to C. chinensis and C. maculatus using artificial seeds made from various proportions of V. nepalensis cotyledon seed powder in V. angularis. (DOC 35 kb)

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Somta, P., Kaga, A., Tomooka, N. et al. Mapping of quantitative trait loci for a new source of resistance to bruchids in the wild species Vigna nepalensis Tateishi & Maxted (Vigna subgenus Ceratotropis). Theor Appl Genet 117, 621–628 (2008). https://doi.org/10.1007/s00122-008-0806-3

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  • DOI: https://doi.org/10.1007/s00122-008-0806-3

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