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Mapping QTL for bruchid resistance in rice bean (Vigna umbellata)

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Abstract

The damage caused to stored seed by bruchids (Callosobruchus maculatus) is considered to be a major production constraint in rice bean (Vigna umbellata). Breeding for genetically determined resistance is the most environmentally benign and cost-effective means to mitigate the losses to bruchid infestation. Here, a screen of rice bean germplasm identified two sources of resistance, and determined the genetic basis of the resistance using a quantitative trait locus (QTL) mapping approach. The two resistant accessions (LRB238 and JP100304) were each crossed to a common susceptible cultivar (LRB26) to generate F2 mapping populations, one of which (LRB238 × LRB26) was genotyped with a range of Vigna sp. microsatellite assays and by sequence related amplified polymorphism (SRAP) fingerprinting. The resulting linkage map comprised ten linkage groups and covered a genetic distance of 872.1 cM with a mean inter-marker distance of 32.05 cM. The subsequent QTL analysis detected the presence of 11 QTL, distributed over all ten linkage groups, most of which were associated with the % damage caused to the seed. Two major QTL, Cmpd1.5 (flanked by the SRAP markers E2M9-270 and E12M7-311) and Cmpd1.6 (flanked by the SRAP marker E7M10-141 and the microsatellite locus CEDG259) mapped within 11.9 cM and 13.0 cM of the flanking markers, respectively, accounted for, 67.3 and 77.4 % of the variance respectively, for % damaged seeds. A bulked segregation analysis carried out in the JP100304 × LRB26 population revealed that the resistance donor harboured some resistance factors not represented in LRB238.

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Acknowledgments

The authors are grateful to the Kirkhouse Trust (Oxford, UK) for funding the senior author’s Ph. D. programme (Grant number KSGIN00135). The constructive advice given by Dr. Robert Koebner (Norwich, UK) is particularly acknowledged. We also thank Dr. Prashanth Mohan Raj, National Bureau of Agriculturally Important Insects for providing the C. m aculatus culture, and NIAS (Japan) for the gift of accession JP100304.

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Authors and Affiliations

  1. UAS, GKVK, Bangalore, India

    Pavithravani B. Venkataramana

  2. NSP, UAS, GKVK, Bangalore, India

    Rame Gowda

  3. Department of Agronomy, Faculty of Agriculture at Kamphaeng Sean Kasetsart University, Nakhon Pathom, 73140, Thailand

    Prakit Somta & Peerasak Srinives

  4. Department of Genetics and Plant Breeding, UAS, GKVK, Bangalore, India

    S. Ramesh & A. Mohan Rao

  5. Seed Science and Technology IIHR, Bangalore, India

    K. Bhanuprakash

  6. IIRR, Hyderabad, Telangana, India

    C. Gireesh

  7. ZARS, VC Farm, Mandya UAS, Bangalore, India

    C. K. Pramila

  8. Post-Doctoral Fellow, AICRP on Pigeonpea, University of Agricultural Sciences, GKVK, Bangalore, Karnataka, 560065, India

    Pavithravani B. Venkataramana

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  1. Pavithravani B. Venkataramana
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Venkataramana, P.B., Gowda, R., Somta, P. et al. Mapping QTL for bruchid resistance in rice bean (Vigna umbellata). Euphytica 207, 135–147 (2016). https://doi.org/10.1007/s10681-015-1551-8

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