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High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

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Abstract

The dominant gene Rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen Pyrenophora graminea is located in the distal region of chromosome arm 1 (7H)S. As the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an F2 population of 1,400 plants (ThibautRdg2a×Mirco). The map included six classes of resistance gene analogues (RGAs) tightly associated with Rdg2a. Rdg2a was delimited to a genetic interval of 0.14 cM between the RGAs ssCH4 and MWG851. Additional markers were generated using the sequence from the corresponding region on rice chromosome 6, allowing delimitation of the Rdg2a syntenic interval in rice to a 115 kbp stretch of sequence. Analysis of the rice sequence failed to reveal any genes with similarity to characterized resistance genes. Therefore, either the rice-barley synteny is disrupted in this region, or Rdg2a encodes a novel type of resistance protein.

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Acknowledgements

The authors thank Nadia Faccini and Donata Pagani for technical assistance. We would like to thank Wolfgang Spielmeyer and Evans Lagudah for the ssCH4 probe and Lene Madsen and Jens Stougaard (University of Aarhus) for the S-9202 DNA sequence. This work was supported by the Italian MiPAF, Project “Protezione delle piante mediante l’uso di marcatori molecolari (PROMAR)” and scheda 7/90 MiPAF. N.C. was funded by Syngenta.

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Author notes

  1. N. C. Collins

    Present address: Australian Centre for Plant Functional Genomics, University of Adelaide, SA 5064, Glen Osmond, Australia

Authors and Affiliations

  1. Istituto sperimentale per la Cerealicoltura, Sezione di Fiorenzuola d’Arda, Via S. Protaso 302, 29017 , Fiorenzuola d’Arda, Italy

    D. Bulgarelli, G. Tacconi, E. Dellaglio, A. M. Stanca & G. Valè

  2. Sainsbury Laboratory, John Innes Centre, Norwich, NR4 7UH, UK

    N. C. Collins

  3. Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6420, USA

    R. Brueggeman & A. Kleinhofs

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  1. D. Bulgarelli
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  8. G. Valè
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Correspondence to G. Valè.

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Communicated by P. Langridge

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Bulgarelli, D., Collins, N.C., Tacconi, G. et al. High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley. Theor Appl Genet 108, 1401–1408 (2004). https://doi.org/10.1007/s00122-003-1557-9

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