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Genetic diversity in the northernmost Oryza rufipogon populations estimated by SSR markers

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Abstract

To estimate genetic diversity of the residual northern populations of Oryza rufipogon, a total of 232 individuals from six populations were analyzed using microsatellites (SSRs). The O. rufipogon populations with different status included three from Dongxiang (Jiangxi Province) and three from Chaling (Hunan Province) in China. The 23 rice SSR primer pairs selected from the RiceGenes Database detected a total of 115 alleles, indicating that all the SSR loci were polymorphic in this study. The total gene diversity was 0.919 in the six O. rufipogon populations, and the Donxiang populations showed higher diversity than the Chaling populations. More significant genetic differentiation and less gene flow were found among the Dongxiang populations than those from Chaling. The two putative introgressed populations showed relatively high genetic variation. One in situ conserved population from Dongxiang had the lowest level of genetic diversity. The re-introduced population from Chaling restored about 90% of the genetic variation, compared with the original source population. It is concluded from these results that a relatively high level of genetic variation resided in the northern O. rufipogon populations and continued efforts of conservation of these populations are needed; and that the conservation of some Chaling and Dongxiang populations has been effective in preventing gene flow from cultivated rice. Introgression of cultivated rice demonstrated significant impacts on genetic variability of the O. rufipogon populations, and should be carefully considered in conserving this wild rice. This study also suggested that re-introduction to its original habitats is an effective approach to restore O. rufipogon populations.

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Acknowledgements

We thank Mr. Guihua Liu and Ji Qian for their assistants in sample collection, and to Mr. M. Reagon of the Ohio State University for his comments on this manuscript. This research was supported by the National Natural Science Foundation of China for Distinguished Young Scholars (Grant No. 30125029), the 863 Program of Ministry of Science and Technology of China (Grant No. 2001AA227141), and Shanghai Commission of Science and Technology (Grant No. 02JC140022).

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  1. Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, China

    Z. P. Song, X. Xu, B. Wang, J. K. Chen & B.-R. Lu

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  1. Z. P. Song
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  2. X. Xu
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  3. B. Wang
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  4. J. K. Chen
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  5. B.-R. Lu
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Correspondence to B.-R. Lu.

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Communicated by J.S. Heslop-Harrison

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Song, Z.P., Xu, X., Wang, B. et al. Genetic diversity in the northernmost Oryza rufipogon populations estimated by SSR markers. Theor Appl Genet 107, 1492–1499 (2003). https://doi.org/10.1007/s00122-003-1380-3

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  • DOI: https://doi.org/10.1007/s00122-003-1380-3

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