Journal of Molecular Medicine

, Volume 92, Issue 2, pp 139–149

Nafcillin enhances innate immune-mediated killing of methicillin-resistant Staphylococcus aureus

Authors

    • Department of PediatricsUniversity of California, San Diego
    • Division of Pediatric Pharmacology & Drug DiscoveryUniversity of California San Diego School of Medicine
  • Cheryl Y. Okumura
    • Department of PediatricsUniversity of California, San Diego
    • Department of BiologyOccidental College
  • Wdee Thienphrapa
    • Department of PediatricsUniversity of California, San Diego
  • Joshua Olson
    • Department of PediatricsUniversity of California, San Diego
  • Poochit Nonejuie
    • Department of Biological SciencesUniversity of California, San Diego
  • Quang Dam
    • Department of PediatricsUniversity of California, San Diego
  • Abhay Dhand
    • Department of MedicineNew York Medical College
  • Joseph Pogliano
    • Department of Biological SciencesUniversity of California, San Diego
  • Michael R. Yeaman
    • David Geffen School of Medicine at UCLA and the Los Angeles Biomedical Research Institute at Harbor–UCLA Medical Center
  • Mary E. Hensler
    • Department of PediatricsUniversity of California, San Diego
  • Arnold S. Bayer
    • David Geffen School of Medicine at UCLA and the Los Angeles Biomedical Research Institute at Harbor–UCLA Medical Center
    • Department of PediatricsUniversity of California, San Diego
    • Skaggs School of Pharmacy & Pharmaceutical SciencesUniversity of California, San Diego
    • Division of Pediatric Pharmacology & Drug DiscoveryUniversity of California San Diego School of Medicine
Original Article

DOI: 10.1007/s00109-013-1100-7

Cite this article as:
Sakoulas, G., Okumura, C.Y., Thienphrapa, W. et al. J Mol Med (2014) 92: 139. doi:10.1007/s00109-013-1100-7

Abstract

Based on in vitro synergy studies, the addition of nafcillin to daptomycin was used to treat refractory methicillin-resistant Staphylococcus aureus (MRSA) bacteremia. Daptomycin is a de facto cationic antimicrobial peptide in vivo, with antistaphylococcal mechanisms reminiscent of innate host defense peptides (HDPs). In this study, the effects of nafcillin on HDP activity against MRSA were examined in vitro and in vivo. Exposures to β-lactam antimicrobials in general, and nafcillin in particular, significantly increased killing of S. aureus by selected HDPs from keratinocytes, neutrophils, and platelets. This finding correlated with enhanced killing of MRSA by whole blood, neutrophils, and keratinocytes after growth in nafcillin. Finally, nafcillin pretreatment ex vivo reduced MRSA virulence in a murine subcutaneous infection model. Despite the lack of direct activity against MRSA, these studies show potent, consistent, and generalized nafcillin-mediated “sensitization” to increased killing of MRSA by various components of the innate host response. The use of nafcillin as adjunctive therapy in MRSA bacteremia merits further study and should be considered in cases refractory to standard therapy.

Key messages

  • Nafcillin has been used as adjunctive therapy to clear persistent MRSA bacteremia.

  • Nafcillin enhances killing of MRSA by a cadre of innate host defense peptides.

  • Nafcillin increases binding of human cathelicidin LL-37 to the MRSA membrane.

  • Nafcillin enhances killing of MRSA by neutrophils.

  • Nafcillin reduces virulence of MRSA in a murine subcutaneous infection model.

Keywords

MRSAInnate immunityBeta-lactamsNafcillinHost defense peptides

Copyright information

© Springer-Verlag Berlin Heidelberg 2013