Abstract
Interleukin-2 (IL-2) is an immunoregulatory cytokine produced by T lymphocytes in response to antigen. It is a potent growth and differentiation factor for several cell-types and is structurally related to the four-helix bundle family of cytokines. Here, we report IL-2 inhibitory potential and computational studies on different series of chalcones, benzothiazepines, semicarbazones, and dihydropyrimidines. These compounds were synthesized in wet lab and were then tested for their potency as IL-2 inhibitors through in vitro T cell proliferation, IL-2 cytokine production as well as their effect on oxidative burst. Compounds that showed significant suppressive activity were further evaluated for their cytotoxicity on normal two cell lines. Most of the chalcones were found to have a powerful inhibitory effect on T-lymphocytes proliferation and cytokine production. Among the aza heterocycles benzothiazepines, benzoxazepines, and benzodiazepinones were found to be the strongest IL-2 inhibitors. Molecular docking and MD simulation studies were carried out to correlate experimental and theoretical results whereby a good correlation was observed which indicated that computational studies could provide an alternate tool for the identification and designing of more potent IL-2 inhibitors.
Similar content being viewed by others
References
Ahsan Ullah, Ansari FL, Ihsan-ul-haq, Nazir S, Mirza B (2007) Combinatorial synthesis, lead identification and antitumor study of chalcone-based positional-scanning library. Chem Biodivers 4:203–214
Akbas E, Furgan A (2008) Studies on reactions of pyrimidine compounds. Microwave-Assisted Synthesis of 1,2,3,4-tetrahydro-2-thioxopyrimidine derivatives. Phosphorus Sulfur Silicon 183:82–89
Ansari FL, Umbreen S, Hussain L, Makhmoor T, Nawaz SA, Lodhi MA, Khan SN, Shaheen F, Choudhary MI, Atta-ur-Rehmam (2005) Syntheses and biological activities of chalcone and 1,5-benzothiazepine derivatives: promising new free-radical scavengers, and cholinesterase, urease, and alpha-glucosidase inhibitors. Chem Biodivers 2:487–496
Ansari FL, Iftikhar F, Ihsan-ul-Haq, Baseer M, Rashid U (2008a) Solid-phase synthesis and biological evaluation of a parallel library of 2,3-dihydro-1,5-benzothiazepines. Bioorg Med Chem 16:7691–7697
Ansari FL, Wadood A, Ahsan Ullah, Iftikhar F, Zaheer-ul-Haq (2008b) In silico studies of urease inhibitors to explore ligand-enzyme interactions. J Enzym Inhib Med Chem 24:151–156
Ansari FL, Baseer M, Iftikhar F, Kulsoom S, Ahsan Ullah, Nazir S, Shaukat A, Ihsan-ul-Haq, Mirza B (2009) Microwave assisted synthesis, antibacterial activity against Bordetella bronchiseptica of a library of 3′-hydroxyaryl and heteroaryl chalcones and molecular descriptors-based SAR. ARKIVOC x:318–332
Ansari FL, Kalsoom S, Zaheer-ul-Haq, Ali Z, Jabeen F (2011) In silico studies on 2,3-dihydro-1,5-benzothiazepines as cholinesterase inhibitors. Med Chem Res 21(9):1–11
Arkin MA, Randal M, DeLano WL, Hyde J, Luong TN, Oslob JD, Raphael DR, Taylor L, Wang J, McDowell RS, Wells JA, Braisted AC (2003) Binding of small molecules to an adaptive protein–protein interface. Proc Natl Acad Sci USA 100:1603–1608
Baseer M, Ansari FL, Ashraf Z (2013) Synthesis, in vitro antibacterial and antifungal activity of some N-acetylated and non-acetylated pyrazolines. Pak J Pharm Sci 26:66–73
Braisted AC, Oslob JD, Delano WL, Hyde J, McDowell RS, Waal N, Yu C, Arkin MR, Raimundo BC (2003) Discovery of a potent small molecule IL-2 inhibitor through fragment assembly. J Am Chem Soc 125:3714
Brown EJ, Schreiber SL (1996) A signalling pathway to translational control. Cell 86:517–520
Chandrasekhar S, Abbas R, Reddy M, Yadav V (2002) Solid-phase synthesis of isoxazolines. J Am Chem Soc 4(6):652–655
Essmann U, Perera L, Berkowitz ML, Darden T, Lee H, Pedersen LG (1995) A smooth particle mesh Ewald method. J Chem Phys 103:8577–8593
Humphrey W, Dalke A, Schulten K (1996) VMD—Visual Molecular Dynamics. J Mol Graph 14:33–38
Jafri L, Ansari FL, Jamil M, Kalsoom S, Qureishi S, Mirza B (2012) Microwave-assisted synthesis and bioevaluation of some semicarbazone. Chem Biol Drug Des 79(6):950–959
Kee SP, Gavriilidis A (2007) Batch versus continuous mg-scale synthesis of chalcone epoxide with soluble polyethylene glycol poly-l-leucine catalyst. J Mol Catal A Chem 263:152–162
Kirkman RL, Barrett LV, Gaulton GN, Kelley VE, Koltun WA, Schoen FJ, Ythier A, Strom TB (1985) The effect of anti-interleukin-2 receptor monoclonal antibody on allograft rejection. Transplantation 40:719–722
Levai A, Szikszai AK (2008) Synthesis of optically active 1,5-benzothiazepines. ARKIVOC I:65–86
Lipinski CA (2000) Drug-like properties and the causes of poor solubility and poor permeability. J Pharmacol Toxicol Methods 44:235–249
Mesaik MA, Jabeen A, Halim SA, Begum A, Khalid A, Shukralla MA, Fatima B, Zaheer-ul-Haq, Choudhary MI (2012) In silico and in vitro immunomodulatory studies on compounds of Lindelofia stylosa. Chem Biol Drug Des 79:290–299
Michael D, David S (2005) Transplant-related immunosuppression: a review of immunosuppression and pulmonary infections. Proc Am Thorac Soc 2:449–455
Molecular operating environment (MOE 2011.10) Chemical Computing Group Inc., Montreal
Mule JJ, Shu S, Schwartz SL, Rosenberg SA (1984) Adoptive immunotherapy of established pulmonary metastases with LAK cells and recombinant interleukin-2. Science 225:1487–1489
Murakawa Y, Takada S, Ueda Y, Suzuki N, Hoshino T, Sakane T (1985) Characterization of T lymphocyte subpopulations responsible for deficient interleukin 2 activity in patients with systemic lupus erythematosus. J Immunol 134:187–195
Nawaz SA, Umbreen S, Kahlid A, Ansari FL, Choudhary MI (2008) Structural insight into the inhibition of acetylcholinesterase by 2,3,4,5-tetrahydro-1,5-benzothiazepines. J Enzym Inhib Med Chem 23:206–212
Nelson BH, Willerford DM (1998) Biology of the interleukin-2 receptor. J Biol Chem 268:13601–13608
Pahwa R, Paradise C, Good RA (1989) Management of a novel immune deficiency with IL-2 therapy. Cancer Treat Rev 16 Suppl A:143–149
Parvez M, Umbreen S, Ansari FL (2003) Derivative of 4-(2-hydroxyphenyl)-2-phenyl-2,3-dihydro-1,5-benzothiazepine. Acta Cryst C59:o298–o301
Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC, Ferrin TE (2004) UCSF Chimera-A visualization system for exploratory research and analysis. J Comput Chem 25:1605–1612
Phadke K, Carlson DG, Gitter BD, Butler LD (1986) Role of interleukin 1 and interleukin 2 in rat and mouse arthritis models. J Immunol 136:4085–4091
Ryckaert JP, Ciccotti G, Berendsen HJC (1977) Numerical integration of the Cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes. J Comput Phys 23:327–341
Saxena S, Nouri-Aria KT, Anderson MG, Eddleston AL, Williams R (1986) Interleukin 2 activity in chronic liver disease and the effect of in vitro alpha-interferon. Clin Exp Immunol 63(3):541–548
Scholz G, Pohl I, Genschow E, Klemm M, Spielmann H (1999) Embryotoxicity screening using embryonic stem cells in vitro correlation to in vivo teratogenicity. Cells Tissues Organs 165:203–211
Sharma V, Sharma KV (2010) Synthesis and biological activity of some 3,5-diarylisoxazoline derivatives: reaction of substituted chalcones with hydroxylamine hydrochloride. Electron J Chem 7(1):203–209
Shen S, Lee W, Lin H, Huang H, Ko C, Yang L, Chen Y (2002) In vitro and in vivo inhibitory activities of rutin, wogonin, and quercetin on lipopolysaccharide-induced nitric oxide and prostaglandin E2 production. Eur J Pharmacol 446:187–194
Sivakumar PM, Ganesan S, Veluchamy P, Doble M (2010) Novel chalcones and 1,3,5-triphenyl-2-pyrazoline derivatives as antibacterial agents. Chem Biol Drug Des 76:407–411
Smith KA (1988) The interleukin-2 gene is expressed in the syncytiotrophoblast of the human placenta. Science 240:1169–1176
Thirunarayanan G, Vanangamudi G (2007) Synthesis of some aryl chalcones using silica- sulphuric acid reagent under solvent free conditions. Electron J Chem 4:90–96
Tilley JW, Chen L, Fry DC, Emerson SD, Powers GD, Biondi D, Varnell T, Trilles R, Guthrie R, Mennona F, Kaplan G, LeMahieu RA, Carson M, Han RJ, Liu CM, Palermo R, Ju G (1997) Identification of a small-molecule inhibitor of the IL-2 IL-2r-alpha receptor interaction which binds to IL-2. J Am Chem Soc 119:7589–7590
Trotter JL, Clifford DB, Anderson CB, Van der Veen RC, Hicks BC, Banks G (1988) Elevated serum interleukin-2 levels in chronic progressive multiple sclerosis. N Engl J Med 318:1206
Waldmann TA, O’Shea J (1998) The use of antibodies against the IL-2 receptor in transplantation. Curr Opin Immunol 10(5):507–512
Wiederrecht GJ, Sabers CJ, Brunn GJ, Martin MM, Dumont FJ, Abraham RT (1995) Mechanism of action of rapamycin: new insights into the regulation of G1-phase progression in eukaryotic cells. Prog Cell Cycle Res 1:53–71
Wu X, Wilairat P, Go Mei-Lin (2002) Antimalarial activity of ferrocenyl chalcones. Bioorg Med Chem Lett 12:2299–2302
Yang Y, Zheng Yu, Xia P, Bastow KF, Nakanishi Y, Kuo HL (2000) Antitumor agents. Part 202: novel 2′-amino chalcones: design, synthesis and biological evaluation. Bioorg Med Chem Lett 10:8699–8701
Zaheer-ul-Haq, Khan W, Kalsoom S, Ansari FL (2010) In silico modeling of the specific inhibitory potential of thiophene-2,3-dihydro-1,5-benzothiazepine against BChE in the formation of β-amyloid plaques associated with Alzheimer’s disease. Theor Biol Med Model 7(1):22–47
Zielasek J, Burkart V, Naylor P, Goldstein A, Kiesel U, Kolb H (1990) Interleukin-2-dependent control of disease development in spontaneously diabetic BB rats. Immunology 69:209–214
Acknowledgments
The authors Farzana Latif Ansari, Zaheer-ul-Haq, and Saima Kalsoom acknowledge the Higher Education Commission, Islamabad, Pakistan for providing financial support to conduct this study.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Kalsoom, S., Rashid, U., Shaukat, A. et al. In vitro and in silico exploration of IL-2 inhibition by small drug-like molecules. Med Chem Res 22, 5739–5751 (2013). https://doi.org/10.1007/s00044-013-0564-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00044-013-0564-x