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Coding variants of TLR2 and TLR4 genes do not substantially contribute to prosthetic joint infection

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Abstract

Objective and design

Prosthetic joint infection (PJI) is a severe complication of total joint arthroplasty (TJA). We conducted a genetic association study that investigated whether selected coding variants of the genes for Toll-like receptors (TLR)2 and TLR4 may contribute to genetic susceptibility for PJI.

Subjects and methods

In total, 350 patients with TJA (98 with PJI/252 without PJI), and 189 unrelated healthy Czech individuals without TJA were enrolled in our study. Three missense polymorphisms of the genes encoding for TLR2 (TLR2 R753Q, rs5743708) and TLR4 (TLR4 D299G, rs4986790 and T399I, rs4986791) were genotyped by “TaqMan” assay.

Results

The frequencies of less common variants for the investigated TLR2/TLR4 polymorphisms in healthy individuals were similar to those observed in other Caucasian populations. Importantly, the distribution of TLR2/TLR4 genotype alleles did not differ between the patients with PJI and the control groups of patients with nonseptic prostheses/healthy individuals.

Conclusion

Our data suggest that structural genetic variants of the receptors TLR2 and TLR4 do not substantially affect the risk of prosthetic joint infection.

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Acknowledgments

We would like to thank Radek Hart, MD, PhD (Department of Orthopaedics, Znojmo, Czech Republic) and Petr Kozak, MD (Department of Orthopaedics, Frydek-Mistek, Czech Republic) and their co-workers for their kind help with recruitment of patients with prosthetic joint infection. The technical assistance of Ms. J. Onderkova and S. Zachova is gratefully acknowledged. Grant support: IGA MZCR NS/10260-3 and CZ.1.05/2.1.00/01.0030.

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Authors and Affiliations

  1. Laboratory of Immunogenomics and Immunoproteomics, Department of Immunology, Faculty of Medicine and Dentistry, Palacky University, I. P. Pavlova str. 6, 775 20, Olomouc, Czech Republic

    Frantisek Mrazek, Anna Stahelova & Martin Petrek

  2. Department of Orthopaedics, Faculty of Medicine and Dentistry, Palacky University and University Hospital, I. P. Pavlova 6, 775 20, Olomouc, Czech Republic

    Jiri Gallo

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  1. Frantisek Mrazek
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  2. Jiri Gallo
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  3. Anna Stahelova
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  4. Martin Petrek
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Correspondence to Frantisek Mrazek.

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Responsible Editor: Andras Falus.

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Mrazek, F., Gallo, J., Stahelova, A. et al. Coding variants of TLR2 and TLR4 genes do not substantially contribute to prosthetic joint infection. Inflamm. Res. 62, 483–487 (2013). https://doi.org/10.1007/s00011-013-0601-8

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  • DOI: https://doi.org/10.1007/s00011-013-0601-8

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