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Phenotypic and genotypic typing ofSalmonella enterica serovar Enteritidis isolates from poultry farms environments in Tunisia

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Abstract

Salmonella enterica serovar Enteritidis is one of the majorSalmonella serovars which may cause animal infections and human salmonellosis. In this study, two hundred forty five samples (faeces, water and environmental swabs) were taken from eight poultry farms localized in different geographical areas of Tunisia. We foundSalmonella serovar Enteritidis (16 strains),Salmonella typhimirium (2),Salmonella scharzengrund (2), andSalmonella braenderup (1).Salmonella Enteritidis strains were characterized by pulsed field gel electrophoresis (PFGE) analysis, plasmid analysis and antibiotic resistance profiles.XbaI PFGE analysis revealed two PFGE types and plasmid profiling identified four plasmid types. The majority of isolates were susceptible to all antibiotic tested. The combined use of phenotypic and genotypic methods indicates the spread of a particularSalmonella Enteritidis clone. This clone is highly related to a major world-wide clone identified in many other countries.

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Authors and Affiliations

  1. Institut de la Recherche Vétérinaire de Tunisie, 20 rue Jebel Lakhdar, Bab Saadoun, 1006, Tunis

    Intissar Gueda, Rafika Debya, Mohamed Salah Abbassi, Leila Soufi & Salah Hamami

  2. Services des Laboratoires, Centre National de Greffe de Moelle Osseuse, Tunis, Tunisie

    Assia Ben Hassen

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  1. Intissar Gueda
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  2. Rafika Debya
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  3. Mohamed Salah Abbassi
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  4. Leila Soufi
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  5. Assia Ben Hassen
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  6. Salah Hamami
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Correspondence to Mohamed Salah Abbassi.

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Gueda, I., Debya, R., Abbassi, M.S. et al. Phenotypic and genotypic typing ofSalmonella enterica serovar Enteritidis isolates from poultry farms environments in Tunisia. Ann. Microbiol. 59, 373–377 (2009). https://doi.org/10.1007/BF03178342

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  • DOI: https://doi.org/10.1007/BF03178342

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