Skip to main content
Log in

Chemical composition effects onto antimicrobial and antioxidant activities of propolis collected from different regions of Turkey

  • Physiology and Metabolism
  • Short Communications
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

Chrysin, apigenin, flavonoids, flavanones, naringenin, ethyl oleate, 3-4-dimethoxy-cinnamic acid and 9-octadecenoic acid were the predominant components of propolis samples collected from different regions of Turkey. The extracts of P3 from Denizli-Başkarci, P5 from Denizli and P7 from Tekirdaĝ had effective antibacterial activities on Gram-negatives. Chrysin, which has antibacterial activity, was found to be high concentration. The extracts of P3, P2B from Aydin and P6 from Konya had much more effective antibacterial activities on Gram-positives. The total antioxidant activity increased with the increasing amount of extracts added to linoleic acid emulsion. All doses of propolis ethanol extract displayed antioxidant activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Aliyazicioglu Y., Deger O., Ovali E., Barlak Y., Hosver I., Tekelioglu Y., Caner Karahan S. (2005). Effects of Turkish pollen and propolis extracts on respiratory burst for K-562 cell lines. Int. Immunopharmacol., 5: 1652–1657.

    Article  CAS  PubMed  Google Scholar 

  • Bankova V., de Castro S.L., Marcucci M.C. (2000). Propolis: Recent advances in chemistry and plant origin. Apidologie, 31: 3–15.

    Article  CAS  Google Scholar 

  • Blonska M., Bronikowska J., Pietsz G., Czuba Z.P., Scheller S., Krol W. (2004). Effects of ethanol extract of propolis (EEP) and its flavones on inducible gene expression in J774A.1 macrophages. J. Ethnopharmacol., 91: 25–30.

    Article  CAS  PubMed  Google Scholar 

  • Brown J.E., Khodr H., Hider R.C., Rice-Evans C.A. (1998). Structural dependence of flavonoid interactions with Cu2 ions: implications for their antioxidant properties. Biochem. J., 330: 1173–1178.

    CAS  PubMed  Google Scholar 

  • Burdock G.A. (1998). Review of the biological properties and toxicity of bee propolis (propolis). Food Chem. Toxicol., 36: 347–363.

    Article  CAS  PubMed  Google Scholar 

  • Burits M., Bucar F. (2000). Antioxidant activity ofNigella sativa essential oil. Phytoter. Res., 14: 323–328.

    Article  CAS  Google Scholar 

  • Castaldo S., Capasso F. (2002). Propolis, an old remedy used in modern medicine. Fitoterapia, 73 (1), S1-S6.

    Article  CAS  PubMed  Google Scholar 

  • Cuendet M., Hostettmann K., Potterat O. (1997). Iridoid glucosides with free radical scavenging properties fromFagraea blumei. Helv. Chim. Acta, 80: 1144–1152.

    Article  CAS  Google Scholar 

  • Choi Y.M., Noh D.O., Cho S.Y., Suh H.J., Kim J.M. (2006). Antioxidant and antimicrobial activities of propolis from several regions of Korea. LWT, 39: 756–761.

    Article  CAS  Google Scholar 

  • Duarte S., Rosalen P.L., Hayacibara M.F., Cury J.A., Bowen W.H., Marquis R.E., Rehder V.L.G., Sartoratto A., Ikegaki M., Koo H. (2006). The influence of a novel propolis on mutans streptococci biofilms and caries development in rats. Arch. Oral Biol., 51: 15–22.

    Article  PubMed  Google Scholar 

  • Duh P.D., Tu Y.Y., Yen G.C. (1999). Antioxidant activity of water oxidative damage of erythrocyte membrane by the flavonoid quercetin and its relation to iron chelating activity. FEBS Lett., 416: 123–129.

    Google Scholar 

  • Grange J.M., Davey R.W. (1990). Antibacterial properties of propolis (bee glue). J. Roy. Soc. Med., 83: 159–160.

    CAS  PubMed  Google Scholar 

  • Gunduz C., Biray C., Kosova B., Yilmaz B., Eroglu Z., Sahin F., Omay S.B., Cogulu O. (2005). Evaluation of Manisa propolis effect on leukemia cell line by telomerase activity. Leukemia Res., 29: 1343–1346.

    Article  CAS  Google Scholar 

  • Hatano T., Edamatsu R., Mori A., Fujita Y., Yasuhara E. (1989). Effect of interaction of tannins with co-existing substances. VI. Effects of tannins and related polyphenols on superoxide anion radical and on DPPH radical. Chem. Pharm. Bull., 37: 2016–2021.

    CAS  Google Scholar 

  • Isla, M.I., Moreno M.I.N., Sampietro A.R., Vattuone M.A. (2001). Antioxidant activity of Argentine propolis extracts. J. Ethnopharmacol., 76: 165–170.

    Article  CAS  PubMed  Google Scholar 

  • Kartal M., Yildiz S., Kaya S., Kurucu S., Topçu G. (2003). Antimicrobial activity of propolis samples from two different regions of Anatolia. J. Ethnopharmacol., 86: 69–73.

    Article  CAS  PubMed  Google Scholar 

  • Keskin N., Hazir S., Baser S.H., Kurkcuoglu M. (2001). Antibacterial activity and chemical composition of Turkish propolis. Z. Naturforsch., 56c: 1112–1115.

    Google Scholar 

  • Kolankaya D., Selamoĝlu G., Sorkun K., Salih B. (2002). Protective effects of Turkish propolis on alcohol-induced serum lipid changes and liver injury in male rats. Food Chem., 78: 213–217.

    Article  CAS  Google Scholar 

  • Koo H., Gomes B.P.F.A., Rosalen P.L., Ambrosano G.M.B., Park Y.K., Cury J.A. (2000).In vitro antimicrobial activity of propolis andArnca montana against oral pathogens. Arch. Oral Biol., 45: 141–148.

    Article  CAS  PubMed  Google Scholar 

  • Kosalec I., Bakmaz M., Pepeljnjak S. (2003). Analysis of propolis from continental and Adriatic regions of Croatia. Acta Pharm., 53: 275–285.

    CAS  PubMed  Google Scholar 

  • Kosalec I., Bakmaz, M., Pepeljnjak, S., Vladimir-Knezevic, S. (2004). Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta Pharm., 54 (1): 65–72.

    CAS  PubMed  Google Scholar 

  • Kujumgiev A., Bankova V., Ignatova A., Popov S. (1993). Antibacterial activity of propolis, some of its components and their analogs. Pharmazie, 48: 785–786.

    CAS  PubMed  Google Scholar 

  • Kujumgiev A., Tsvetkova I., Serkedjieva Yu., Bankova V., Christov R., Popov S. (1999). Antibacterial, antifungal, and antiviral activity of propolis of different geographic origin. J Ethnopharmacol., 64: 235–240.

    Article  CAS  PubMed  Google Scholar 

  • Kumazawa S., Hamasaka T., Nakayama T. (2004). Antioxidant activity of propolis of various geographic origins. Food Chem., 84: 329–339.

    Article  CAS  Google Scholar 

  • Marcucci M.C., (1995). Propolis: chemical composition, biological properties and therapeutic activity. Apidologie, 26: 83–89.

    Article  CAS  Google Scholar 

  • Marcucci M. C., Ferreres F., Garcia-Viguera C., Bankova V.S., De Castro S.L., Dantas A.P., Valente P.H.M., Paulino N. (2001). Phenolic compounds from Brazilian propolis with pharmacological activities. J. Ethnopharmacol., 74: 105–112.

    Article  CAS  PubMed  Google Scholar 

  • Nieva M.M.I., Isla M.I., Cudmani N.G., Vattuone M.A., Sampietro A.R. (1999). Screening of antibacterial activity of Amaicha del Valle (Tucuman, Argentina) propolis. J. Ethnopharmacol., 68: 97–102.

    Article  Google Scholar 

  • Oktay M., Gülçin I., Küfrevioglu Ö.I. (2003). Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. Lebensm.-Wiss., Technol., 36: 263–271.

    Article  CAS  Google Scholar 

  • Özcan M. (2000). Use of propolis extract as a natural antioxidant for plant oils. Grasas y Aceites, 51 (4): 251–253.

    Article  Google Scholar 

  • Park Y.K., Koo M.H., Ikegaki M., Contado J.L. (1997). Investigations of the flavonoid aglycones of propolis collected byApis mellifera in Brazil. Arq. Biol. Technol., 40: 97–106.

    CAS  Google Scholar 

  • Park Y.K., Koo M.H., Ikegaki M., Cury C.A., Rosalen P.L. (1998). Effects of propolis onStreptococcus mutans, Actinomyces naeslundii andStaphylococcus aureus, Revista de Microbiologia, 29 (2): 143–148.

    Google Scholar 

  • Pepeljnjak S., Kosalec I. (2004). Galangin expresses bactericidal activity against multiple-resistant bacteria: MRSA,Enterecoccus spp. andPseudomonas aeruginosa. FEMS Microbiol. Lett., 240: 111–116.

    Article  CAS  PubMed  Google Scholar 

  • Popova M., Silici S., Kaftanoglu O., Bankova V. (2005). Antibacterial activity of Turkish propolis and its qualitative and quantitative chemical composition. Phytomedicine, 12: 221–228.

    Article  CAS  PubMed  Google Scholar 

  • Pratt, D.E. (1992). Natural antioxidants from plant material. In; Huang M.T., Ho C.T., Lee, C.Y., Eds., Phenolic Compounds in Food and their Effect on Health. II: Antioxidants and Cancer Prevention. American Chemical Society, Washington, DC, pp. 54–71.

    Chapter  Google Scholar 

  • Prytzyk E., Dantas A.P., Salomao K., Pereira A.S., Bankova V.S., De Castro S.L., Aquino Neto F.R. (2003). Flavonoids and trypanocidal activity of Bulgarian propolis. J. Ethnopharmacol., 88: 189–193.

    Article  CAS  PubMed  Google Scholar 

  • Salomao K., Dantas A.P., Borba C.M., Campos L.C., Machado D.G., Aguino N.F.R., de Castro S.L. (2004). Chemical composition and microbicidal activity of extracts from Brazilian, and Bulgarian propolis. Lett. Appl. Microbiol., 38: 87–92.

    Article  CAS  PubMed  Google Scholar 

  • Sforcin J.M., Fernandes Jr. A., Lopes C.A.M., Bankova V., Funari S.R.C. (2000). Seasonal effect on Brazil propolis antibacterial activity. J. Ethnopharmacol., 73: 243–249.

    Article  CAS  PubMed  Google Scholar 

  • Silici S., Kutluca S. (2005). Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J. Ethnopharmacol. 99: 69–73.

    Article  CAS  PubMed  Google Scholar 

  • Slinkard K., Singleton V.L. (1977). Total phenol analyses: automation and comparison with manual methods. Am. J. Enol. Viticult., 28: 49–55.

    CAS  Google Scholar 

  • Sonmez S., Kirilmaz L., Yucesoy M., Yucel B., Yilmaz B. (2005). The effect of bee propolis on oral pathogens and human gingival fibroblasts. J. Ethnopharmacol., 102: 371–376.

    Article  PubMed  Google Scholar 

  • Sorkun K., Suer B., Salih B. (2001). Determination of chemical composition of Turkish propolis. Z. Naturforsch. C., 56 (7–8): 666–668.

    CAS  PubMed  Google Scholar 

  • Sugihara N., Arakawa T., Ohnishi M., Furuno K. (1999). Anti- and pro-oxidative effects of flavonoids on metal-induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes loaded with a-linoleic acid. Free Radical Biol. Med., 27: 1313–1323.

    Article  CAS  Google Scholar 

  • Sahinler N., Kaftanoĝlu O. (2005). Natural product propolis: Chemical composition. Nat. Prod. Res., 19 (2): 183–188.

    Article  CAS  PubMed  Google Scholar 

  • Uzel A., Sorkun K., Önçaĝ Ö, Çoĝulu D., Gençay Ö., Salih B. (2005). Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiol. Res., 160: 189–195.

    Article  CAS  PubMed  Google Scholar 

  • Vinson J.A., Yong H., Xuchui S., Zubik L. (1998). Phenol antioxidant quantity and quality in foods: vegetables. J. Agric. Food Chem., 46: 3630–3634.

    Article  CAS  Google Scholar 

  • Wang B.J., Lien Y.H., Yu Z.R. (2004). Supercritical fluid extractive fractionation study of the antioxidant activities of propolis. Food Chem., 86: 237–243.

    Article  CAS  Google Scholar 

  • Yen G.C., Duh P.D., Tsai C.L. (1993). Relationship between antioxidant activity and maturity of peanut hulls. J. Agric. Food Chem., 41: 67–70.

    Article  CAS  Google Scholar 

  • Yildinm Z., Hacievliyagil S., Kutlu O.N., Aydin N.E., Kurkcuoglu M., Iraz M., Durmaz R. (2004). Effect of water extract of Turkish propolis on tuberculosis infection in guinea-pigs. Pharmacol. Res., 49: 287–292.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Nazime Mercan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mercan, N., Kivrak, I., Duru, M.E. et al. Chemical composition effects onto antimicrobial and antioxidant activities of propolis collected from different regions of Turkey. Ann. Microbiol. 56, 373–378 (2006). https://doi.org/10.1007/BF03175035

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03175035

Key words

Navigation