Skip to main content
SpringerLink
Log in

Effect of root canal sealers on mouse peritoneal macrophage functions

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Three root canal filling materials,viz. calcium hydroxide-based cement (Apexit®, resin-based cement (AH-plus®) and glass-ionomer based material (Ketac Endo®) were tested for their influence on several functions of peritoneal macrophages from Balb/c mice. Macrophage functions were evaluated by the adherence, phagocytic, candidacidal and Nitro blue tetrazolium-dye assays. Ketac-Endo® enhanced all macrophage functions in the first 2 d (p≤0.05), when compared to the positive control, but this effect had changed after 7 and 14 d, causing inhibition of these functions. Other materials suppressed substrate adherence capacity and phagocytosis, while significantly stimulating macrophage microbicidal activity (p≤0.05) in a time-dependent manner.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AI:

adherence index

MΦ:

macrophage(s)

NBT:

Nitroblue tetrazolium

PI:

phagocytic index

TGC:

thioglycollate

References

  • Hauman C.H.J., Love R.M.: Biocompatibility of dental materials in contemporary endodontic therapy: part 2. Root canal filling materials.Internat.Endod.J. 36, 147 (2003).

    Article  CAS  Google Scholar 

  • Huang F.M., Tai K.W., Chou M.Y., Chang Y.C.: Cytotoxicity of resin-, zinc oxide eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells.Internat.Endod.J. 35, 153–158 (2002a).

    Article  Google Scholar 

  • Huang T.H., Yang J.J., Li H., Kao C.T.: The biocompatibility evaluation of epoxy resin based root canal sealersin vitro.Biomaterials 23, 77–83 (2002b).

    Article  PubMed  Google Scholar 

  • Jimenez-Rubio A., Segura J.J.: The effect of the bleaching agent sodium perborate on macrophage adhesionin vitro: implications in external cervical root resorption.J.Endod. 24, 229–232 (1998).

    Article  PubMed  CAS  Google Scholar 

  • Kaplan A.E., Goldberg F., Artaza L.P., de Silvio A., Macchi R.L.: Disintegration of endodontic cements in water.J.Endod. 23, 439–441 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Liapatas S., Nakou M., Rontogianni D.: Inflammatory infiltrate of chronic periradicular lesions: an immunohistochemical study.Internat.Endod.J. 36, 464–471 (2003).

    Article  CAS  Google Scholar 

  • Miletić I., Anić I., Karlović Z., Maršan T., Pezelj-Ribarić S., Osmak M.: Cytotoxic effects of the four root filling materials.Endod.Dent.Traumatol. 16, 287–290 (2000).

    Article  PubMed  Google Scholar 

  • Miletić I., Anić I., Pezelj-Ribarić S., Jukić S.: Leakage of five root canal sealers.Internat.Endodon. 32, 415–418 (1999).

    Article  Google Scholar 

  • Miletić I., Cevcić N., Anić I., Borcić J., Karlović Z., Osmak M.: The cytotoxicity of Roeko Seal and AH plus compared during different setting periods.J.Endod. 31, 307–309 (2005).

    Article  PubMed  Google Scholar 

  • de Oliveira Mendes S.T., Ribeiro Sobrinho A.P., de Carvalho A.T., de Souza Cortes M.I., Vieira L.Q.:In vitro evaluation of the cytotoxicity of two root canal sealers on macrophage activity.J.Endod. 29, 95–99 (2003).

    Article  PubMed  Google Scholar 

  • Perassi F.T., Filho I.B., Berbert F.L., Carlos I.Z., de Toledo Leonardo R.: Secretion of tumor necrosis factor-α by mouse peritoneal macrophages in the presence of dental sealers, sealapex and endomethasone.J.Endod. 30, 534–537 (2004).

    Article  PubMed  Google Scholar 

  • Pezelj-Ribarić S., Brekalo I., Abram M., Dorić M., Miletić I., Karlović Z.: Influence of calcium hydroxide root-canal sealer on microbial growthin vitro.Folia Microbiol. 47, 458–460 (2002).

    Article  Google Scholar 

  • Pommel L., About I., Pashley D., Camps J.: Apical leakage of four endodontic sealers.J.Endod. 29, 208–210 (2003).

    Article  PubMed  Google Scholar 

  • Sedeghein A., Bolhari B., Sarafnejad A.: A comparison of the effect of three endodontic sealers on adherence of mouse peritoneal macrophages.J.Calif.Dent.Assoc. 29, 673–677 (2001).

    Google Scholar 

  • Segura J.J., Liamas R., Jimenez Rubio-Manzanares A.: Calcium hydroxide inhibits substrate adherence capacity of macrophages.J.Endod. 23, 444–447 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Spangberg L.: Instruments, materials and devices, pp. 548–558 in S. Cohen, R.C. Burns:Pathways of the Pulp. Mosby, St.Louis (USA) 2002.

    Google Scholar 

  • Tomazić-Jezić V.J., Merritt K., Umbreit T.H.: Significance of the type and the size of biomaterial particles on phagocytosis and tissue distribution.J.Biomed.Mater.Res. 55, 523–529 (2001).

    Article  PubMed  Google Scholar 

  • Trindade M.C.D., Lind M., Sun D., Schurman D.J., Goodman S.B., Smith R.L.:In vitro reaction to orthopedic biomaterials by macrophages and lymphocytes isolated from patients undergoing revision surgery.Biomaterials 22, 253–259 (2001).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Dental Pathology, University of Rijeka, 51 000, Rijeka, Croatia

    I. Brekalo

  2. Department of Oral Medicine, University of Rijeka, 51 000, Rijeka, Croatia

    S. Pezelj-Ribarić

  3. Department of Microbiology, Medical Faculty, University of Rijeka, 51 000, Rijeka, Croatia

    M. Abram

  4. Department of Oral and Maxillofacial Surgery, University Hospital Rijeka, Croatia

    V. Ahel

Authors
  1. I. Brekalo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Pezelj-Ribarić
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Abram
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. Ahel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. Brekalo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brekalo, I., Pezelj-Ribarić, S., Abram, M. et al. Effect of root canal sealers on mouse peritoneal macrophage functions. Folia Microbiol 52, 95–98 (2007). https://doi.org/10.1007/BF02932146

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02932146

Keywords

Search

Navigation