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Invertebrate tissue culture as a tool to study insect transmission of plant viruses

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Summary

Invertebrate tissue culture is an underutilized resource in the study of insect-transmitted plant viruses. Since the establishment of leafhopper vector monolayers over 20 yr ago, these continuous cultures were used primarily to assay the infectivity of plant viruses that replicate in their insect vectors and to elucidate the viral determinants for transmission of wound tumor phytoreovirus and potato yellow dwarf rhabdovirus. Primary aphid cultures were used to study the uptake of viruses and viral accumulation. The biggest potential for use of insect cell culture in the future is in studying the molecular biology of propagative viruses. Although leafhopper cell lines do not support the same virus-vector specificity that is found in vivo, the cell lines have some advantage over the use of whole insects in the ease with which viral transcripts and mutant viruses can be uniformly introduced into cells. Existing leafhopper monolayer systems could facilitate studies on the influence of the vector’s genetic background on viral replication. Cultures derived from salivary glands might be useful to study the insect receptors that recognize the viral capsid. Cell lines from planthoppers, thrips, and eriophid mite vectors would allow research on the biological and molecular characterization of poorly characterized plant viruses.

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References

  • Adam, G. Plant virus studies in insect vector cell cultures. In: Mayo, M. A.; Harrap, K. A., eds. Vectors in virus biology. London: Academic Press; 1984:37–62.

    Google Scholar 

  • Adam, G.; Sander, E. Isolation and culture of aphid cells for the assay of insect-transmitted plant viruses. Virology 70:502–508; 1976.

    Article  PubMed  CAS  Google Scholar 

  • Ammar, E.-D.; Nault, L. R. Assembly and accumulation sites of maize mosaic virus in its planthopper vector. Intervirology 24:33–41; 1985.

    PubMed  CAS  Google Scholar 

  • Bar-Joseph, M.; Marcus, R.; Lee, R. F. The continuous challenge of citrus tristeza virus control. Ann. Rev. Phytopathol. 27:291–316; 1989.

    Article  Google Scholar 

  • Black, L. M. Vector cell monolayers and plant viruses. Adv. Virus Res. 25:191–271; 1979.

    PubMed  CAS  Google Scholar 

  • Brooks, M. A. Nutrition, cell culture and symbiosis of leafhoppers and planthoppers. In: Nault, L. R.; Rodriguez, J. G., eds. The leafhoppers and planthoppers. New York: John Wiley & Sons, Inc.; 1985:195–216.

    Google Scholar 

  • Caciagli, P.; Casetta, A. Maize rough dwarf (reoviridae) in its planthopper vectorLaodelphax striatellus in relation to vector infectivity. Ann. Appl. Biol. 109:337–344; 1986.

    Google Scholar 

  • Chen, M.; Shikata, E. Morphology and intracellular localization of rice transitory yellowing virus. Virology 46:786–796; 1971.

    Article  PubMed  CAS  Google Scholar 

  • Chen, M.; Shikata, E. Electron microscopy and recovery of rice transitory yellowing virus from its leafhopper vector,Nephotettix cincticeps. Virology 47:483–486; 1972.

    Article  PubMed  CAS  Google Scholar 

  • Chui, R. J.; Liu, H. Y.; MacLeod, R., et al. Potato yellow dwarf virus in leafhopper cell culture. Virology 40:387–396; 1970.

    Article  Google Scholar 

  • Conti, M. Transmission of Plant viruses by leafhoppers and planthoppers. In: Nault, L. R.; Rodriguez, J. G., eds. The leafhoppers and planthoppers. New York: John Wiley & Sons, Inc.; 1985:289–307.

    Google Scholar 

  • Dall, D. J.; Anzola, J. V.; Xu, Z., et al. Structure-specific binding of wound tumor virus transcripts by a host factor: involvement of both terminal nucleotide domains. Virology 179:599–608; 1990.

    Article  PubMed  CAS  Google Scholar 

  • Duffus, J. E.; Flock, R. A. Whitefly-transmitted disease complex of the desert southwest. Calif. Agric. 36:4–6; 1982.

    Google Scholar 

  • Falk, B. W.; Tsai, J. H. Assay for maize stripe virus-infected plants by using antiserum produced to a purified noncapsid protein. Phytopathology 73:1259–1262; 1983.

    CAS  Google Scholar 

  • Francki, R. I. B.; Boccardo, G. The plant reoviridae. In: Joklik, W. K., ed. The reoviridae. New York: Plenum Press; 1983:505–563.

    Google Scholar 

  • Gaedigk-Nitschko, K.; Adam, G.; Mundry, K. W. Role of the spike protein from potato yellow dwarf virus during infection of vector cell monolayers. In: Mitsuhashi, J., ed. Invertebrate cell system applications, vol. 2. Boca Raton, FL: CRC Press, Inc.; 1989:91–97.

    Google Scholar 

  • Gamez, R.; Leon, P. Ecology and evolution of a neotropical leafhoppervirus-maize association. In: Nault, L. R.; Rodriguez, J. G., eds. The leafhoppers and planthoppers. New York: John Wiley & Sons, Inc.; 1985:331–350.

    Google Scholar 

  • Gildow, F. E. Transcellular transport of barley yellow dwarf virus into the hemocoel of the aphid vector,Rhopalosiphum padi. Phytopathology 75:292–297; 1985.

    Google Scholar 

  • Gingery, R. E. The rice stripe virus group. In: Milne, R. G., ed. The plant viruses, vol. 4. New York: Plenum; 1988:297–329.

    Google Scholar 

  • Hsu, H. T.; McBeath, J. H.; Black, L. M. The comparative susceptibilities of cultured vector and nonvector leafhopper cells to three plant viruses. Virology 81:257–262; 1977.

    Article  PubMed  CAS  Google Scholar 

  • Huiet, L.; Klaassen, V. A.; Tsai, J. H., et al. Identification and sequence analysis of the maize stripe virus major noncapsid protein gene. Virology 179:862–866; 1990.

    Article  PubMed  CAS  Google Scholar 

  • Huiet, L.; Klaassen, V. A.; Tsai, J. H., et al. Nucleotide sequence and RNA hybridization analyses reveal an ambisense coding strategy for maize stripe virus RNA 3. Virology 182:47–53; 1991.

    Article  PubMed  CAS  Google Scholar 

  • International Committee on the Taxonomy of Viruses. Classification and nomenclature of viruses. Fifth report of the ICTV. In: Francki, R. I. B.; Fauquet, C. M.; Knudson, D. L., et al., eds. Archives of virology, suppl. 2. New York: Springer Verlag/Wien; 1991.

    Google Scholar 

  • Jackson, A. O.; Francki, R. I. B.; Zuidema, D. Biology structure and replication of plant rhabdoviruses. In: Wagner, R., ed. The rhabdoviruses. New York: Plenum Press; 1987:427–507.

    Google Scholar 

  • Kimura, I. Monolayer cell cultures of leafhopper vectors, and their inoculation with a rice virus. In: Mitsuhashi, J., ed. Invertebrate cell system applications, vol. 2. Baco Raton, FL: CRC Press, Inc.; 1989:109–115.

    Google Scholar 

  • Kitajima, E. W.; Gámez, R. Electron microscopy of maize rayado fino virus in the internal organs of its leafhopper vector. Intervirology 19:129–134; 1983.

    PubMed  CAS  Google Scholar 

  • Lastra, J. R.; Esparza, J. Multiplication of vesicular stomatitis virus in the leafhopperPeregrinus maidis (Ashm), a vector of a plant rhabdovirus. J. Gen. Virol. 32:139–142; 1976.

    PubMed  CAS  Google Scholar 

  • Maramorosch, K. Plant pathology applications. In: Maramorosch, K., ed. Invertebrate tissue culture. Research applications. London: Academic Press; 1976:305–316.

    Google Scholar 

  • Matthews, R. E. F. Plant virology. New York: Academic Press; 1991.

    Google Scholar 

  • Mitsuhashi, J. Use of invertebrate cell culture for the study of plant viruses. In: Vago, C., ed. Invertebrate tissue culture, vol. 2. London: Academic Press; 1972:343–360.

    Google Scholar 

  • Nault, L. R.; Ammar, E. D. Leafhopper and planthopper transmission of plant viruses. Ann. Rev. Entomol. 34:503–529; 1989.

    Article  Google Scholar 

  • Nault, L. R.; Gingery, R. E.; Gordon, D. T. Leafhopper transmission and host range of maize rayado fino virus. Phytopathology 70:709–712; 1980.

    Article  Google Scholar 

  • O’Loughlin, G. T.; Chambers, T. C. The systemic infection of an aphid by a plant virus. Virology 33:262–271; 1967.

    Article  PubMed  CAS  Google Scholar 

  • Peter, D.; Black, L. M. Infection of primary cultures of aphid cells with a plant virus. Virology 40:847–853; 1970.

    Article  Google Scholar 

  • Rathgeber, J.; Adam, G.; Mundry, K.-W. Protein synthesis in vector cell monolayers after infection with potato yellow dwarf virus. In: Mitsuhashi, J., ed. Invertebrate cell system applications, vol. 2. Boca Raton, FL: CRC Press, Inc.; 1989:99–108.

    Google Scholar 

  • Reddy, D. V. R.; Black, L. M. Production of wound-tumor virus and woundtumor soluble antigen in the insect vector. Virology 30:551–561; 1966.

    Article  PubMed  CAS  Google Scholar 

  • Reddy, D. V. R.; Black, L. M. Isolation and replication of mutant populations of wound tumor virions lacking certain genome segments. Virology 80:336–346; 1977.

    Article  PubMed  CAS  Google Scholar 

  • Shikata, E. Cytopathological changes in leafhopper vectors of plant viruses. In: Maramorosch, K.; Harris, K. F., ed. Leafhopper vectors and plant disease agents. New York: Academic Press; 1979:309–325.

    Google Scholar 

  • Slykhuis, J. T. Vector and host relations of North American wheat striate mosaic virus. Can. J. Bot. 41:1171–1185; 1963.

    Article  Google Scholar 

  • Vidano, C. Phases of maize rough dwarf virus multiplication in the vectorLaodelphax striatellus (Fallen). Virology 41:218–232; 1970.

    Article  PubMed  CAS  Google Scholar 

Download references

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  1. Department of Plant Pathology, University of California, 92521, Riverside, California

    Rebecca Creamer

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  1. Rebecca Creamer
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Creamer, R. Invertebrate tissue culture as a tool to study insect transmission of plant viruses. In Vitro Cell Dev Biol - Animal 29, 284–288 (1993). https://doi.org/10.1007/BF02633956

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