Summary
Human diploid cultures are widely used for the study of aging. We have developed quantitative cell culture procedures that include optimization of culture conditions and determination of replicative age by direct cell count. Using these procedures we are able to determine reproducibly the stage in the replicative life history of diploid cell cultures.
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Cristofalo, V.J., Charpentier, R. A standard procedure for cultivating human diploid fibroblastlike cells to study cellular aging. Journal of Tissue Culture Methods 6, 117–121 (1980). https://doi.org/10.1007/BF02082862
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DOI: https://doi.org/10.1007/BF02082862