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Inhibition of fading in fluorescence microscopy of fixed cells

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Summary

Following Hirschfeld et al., dithionite was added to mounting media to inhibit fluorescence fading. Excellent response is reported for fluorescein, acridine orange, 33258 Hoechst, acriflavine, berberine (and ethidium bromide), but not for quinacrine.

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Authors and Affiliations

  1. Department of Physics, Ben Gurion University of the Negev, Beer Sheva, Israel

    D. Gill

Authors
  1. D. Gill
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Additional information

Acknowledgment. The author is grateful to Dr T. Hirschfeld of Block Engineering, Cambridge, Mass., USA, for an illuminating introduction to his work.

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Gill, D. Inhibition of fading in fluorescence microscopy of fixed cells. Experientia 35, 400–401 (1979). https://doi.org/10.1007/BF01964376

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  • DOI: https://doi.org/10.1007/BF01964376

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