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25-hydroxyvitamin D, 24, 25-dihydroxyvitamin D and 1,25-dihydroxyvitamin D in human cerebrospinal fluid

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Summary

Samples of CSF and plasma were obtained simultaneously from 46 adult patients who had no endocrine disorders and were undergoing routine diagnostic lumbar puncture because of suspected or proved prolapse of a disc. Concentrations of 25-OHD, 24,25(OH)2D and 1,25(OH)2D were measured. The samples were purified by column chromatography and fractionated by HPLC. In the appropriate fractions the vitamin D metabolites were measured by PBA, and cytoreceptor assay. The results were as follows (median, range in brackets): 25-OHD in CSF 8.3 ng/ml (2.0–24.8), in plasma 14.5 ng/ml (7.0–36.0). 24,25(OH)2D in CSF 1.8 ng/ml (0.3–4.6) and 2.5 ng/ml (0.4–4.7) in plasma. 1.25(OH)2 D in CSF 25.0 pg/ml (2.2–39.0) and 31.0 pg/ml (10.1–55.0) in plasma. The correlations between plasma and CSF concentrations were as follows: 25-OHDr=0.479 (P<0.001); 24,25(OH)2Dr=0.815 (P<0.001) and for 1.25(OH)2Dr=0.497 (P<0.001).

Our findings showed vitamin D metabolites to be present in human CSF.

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Abbreviations

Ca:

Calcium

CSF:

Cerebrospinal fluid

Vitamin D3 :

Cholecalciferol

CPM:

Counts per min

24, 25 (OH)2D:

24, 25-dihydroxyvitamin D3

1,25(OH)2D:

1,25-dihydroxyvitamin D3

Vitamin D2 :

Ergocalciferol

HPLC:

High-pressure liquid chromatography

25OHD:

25-hydroxyvitamin D3

PTH:

Parathyroid hormone

PBA:

Protein binding assay

RIA:

Radioimmunoassay

D-CaBP:

Vitamin D dependent calcium-binding protein

References

  1. Anderson DC, Cooper AF, Naylor GJ (1968) Vitamin D-intoxication with hypernatremia, potassium, and water depletion, and mental depression. Br Med J 4:744–746

    Google Scholar 

  2. Anderson J (1968) Psychiatric aspects of primary hyperparathyroidism. Proc R Soc Med 61:1123–1124

    Google Scholar 

  3. Arieff AI, Massry SG (1974) Calcium metabolism of brain in acute renal failure. J Clin Invest 53:387–392

    Google Scholar 

  4. Brainbridge KG, Miller JJ (1982) Calcium binding distribution in rat brain. Brain Res 239:519–525

    Google Scholar 

  5. Balabanov S, Hanle J, Richter HP, Antoniadis G, Teller WM (1984) 1,25 Dihydroxyvitamin D3 in human cerebrospinal fluid. Acta Endocrinol 105 [Suppl 264 (Abstract)]: × 11

  6. Boyle IT, Gray RW, Omdahl JL, DeLuca HF (1972) Calcium control of the in vivo biosynthesis of 1,25-dihydroxyvitamin D3: Nicolaysen's endogenous factor. In: Taylors (ed) Endocrinology 1971 Proc 3rd International Symposium. Heinemann Medical, London, pp 468–479

    Google Scholar 

  7. Carman JS, Wyatt RJ (1979) Calcium: bivalent cation in the bivalent psychoses. Biol Psychiatry 14:295–336

    Google Scholar 

  8. Chaplin H, Clark LD, Ropes MW (1951) Vitamin D intoxication. Am J Med Sci 221:369–378

    Google Scholar 

  9. Christakos S, Brunette MG, Norman AW (1981) Localization of immunoreactive vitamin D-dependent calcium binding in chick nephron. Endocrinology 109:322–324

    Google Scholar 

  10. DeLuca HF (1979) Vitamin D. Metabolism and function. In: Gross F, Grumbach MM, Labhardt A, Lipsett MB, Mann T, Samuels LT, and Zander J (eds) Monographs on Endocrinology. Springer, Berlin Heidelberg New York, p 45

    Google Scholar 

  11. Eitinger L (1942) Hyperparathyroidism with mental changes. Nord Med 14:1581–1585

    Google Scholar 

  12. Fairney A, Turner C, Baggilioni EG, Uskokovic MR (1977) Development of a radioimmunoassay for vitamin D. In: Norman AW, Schaefer K, Coburn JW, DeLuca HF, Fraser D, Grigoleit H-G, von Herrath D (eds) Vitamin D, biochemical, chemical, and clinical aspects related to calcium metabolism. De Gruyter, Berlin, p 459

    Google Scholar 

  13. Fitz TE, Hallman BL (1952) Mental changes associated with hyperparathyroidism. AMA Arch Int Med 89:547–551

    Google Scholar 

  14. Garabedian M, Holick MF, DeLuca HF, Boyler IT (1972) Control of 25-hydroxycholecalciferol metabolism by parathyroid glands. Proc Nat Acad Sci 69:1673–1676

    Google Scholar 

  15. Haddad JG, Chyu KJ (1971) Competitive protein-binding radioassay for 25-hydrocholecalciferol. J Clin Endocrinol 33:992–995

    Google Scholar 

  16. Horst RW, DeLuca HF, Jorgenson N (1978) Effect of age on calcium absorption and accumulation of 1,25-dihydroxyvitamin D3 in intestinal mucosa of rats. Metab Bone Dis Rel Res 1:29–34

    Google Scholar 

  17. Howard JE, Meyer RJ (1948) Intoxication with vitamin D. J Clin Endocrinol 8:895–910

    Google Scholar 

  18. Hughes RE, Hurley RJ, Jones PR (1971) The retention of ascorbic acid by guinea pig tissues. Br J Nutr 26:433–438

    Google Scholar 

  19. Idler DR, Baumann CA (1952) Skin sterols II. Isolation of Δ7-cholesterol. J Biol Chem 195:623–628

    Google Scholar 

  20. Jande SS, Maler L, Lawson DEM (1981) Immunohistochemical mapping of vitamin D-dependent calcium-binding protein in brain. Nature 294:765–767

    Google Scholar 

  21. Keck E, Durdel R, Schweikert HU, Meier W, Hennes U, Krück F, Krüskemper HL (1980) Mettabolism of 25-hydroxycholecalciferol in human spongiosa. 6th Int Congress of Endocrinology, Melbourne. Program and abstracts, p 254

  22. Kremer R, Herscovitch P, Goltzman D (1980) Detection of 25-hydroxyvitamin D in human cerebrospinal fluid. Clin Res 28:692 (Abstract)

    Google Scholar 

  23. Lehrer GM, Levit MF (1960) Neuropsychiatric presentation of hypercalcemia. J Mt Sinai Hosp 27:10–18

    Google Scholar 

  24. Malluche HH, Henry H, Meyer WA, Sherman D, Massry SG, Norman AW (1979) Effects and interaction of 1,25-(OH2)D3 and 24,25-(OH)2D3 on bone. In: Norman AW, Schaefer K, von Herrat D, Grigoleit HG, Coburn JW, DeLuca HF, Mawer EB, Suda T (eds) Vitamin D, basic research and its clinical application. De Gruyter, Berlin, p 425

    Google Scholar 

  25. Mann GW, Newton P (1975) The membrane transport of ascorbic acid. Ann NY Acad Sci 258:243–252

    Google Scholar 

  26. Manolagas SC, Culler FL, Howard JE, Brickman AS, Deftos LJ (1983) The cytoreceptor assay for 1,25-dihydroxy vitamin D and its application to clinical studies. J Clin Endocrinol Metab 56:751–760

    Google Scholar 

  27. Martin GR, Mecca CE (1961) Studies on the distribution of L-ascorbic acid in the rat. Arch Biochem Biophys 93:110–114

    Google Scholar 

  28. Oliver WA (1939) Acute hyperparathyroidism. Lancet 2:240–244

    Google Scholar 

  29. Patterson JW, Mastin DW (1951) Some effects of dehydroascorbic acid on the central nervous system Am J Physiol 167:119–126

    Google Scholar 

  30. Ponchon G, DeLuca HF (1969) The role of the liver in the metabolism of vitamin D. J Clin Invest 48:1273–1279

    Google Scholar 

  31. Rapoport SI (1976) Blood-brain barrier in physiology and medicine. Raven Press, New York, p 177–207

    Google Scholar 

  32. Rasmussen H, DeLuca HF, Arnaud C, Hawker C, von Stedingk M (1963) The relationship between vitamin D and parathyroid hormone. J Clin Invest 42:1940–1946

    Google Scholar 

  33. Riggs BL, Gallagher JC, DeLuca HF (1979) Osteoporosis and age-related osteopenia: evaluation of possible role of vitamin D endocrine system in pathogenesis of impaired intestinal calcium absorption. In: Norman AW, Schaefer K, von Herrath D, Grigoleit H-G, Coburn JW, DeLuca HF, Mawer EB, Suda T (eds). De Gruyter, Berlin, p 107

    Google Scholar 

  34. Roth J, Baetens D, Norman AW, Garcia-Segura L-M (1981) Specific neurons in chick central nervous system stain with an antibody against chick intestinal vitamin D-dependent calcium-binding protein. Brain Res 222:452–457

    Google Scholar 

  35. Spector R, Lorenzo AV (1973) Ascorbic acid homeostasis in the central nervous system. Am J Physiol 225:757–763

    Google Scholar 

  36. Spector R (1976) Thiamine transport in the central nervous system. Am J Physiol 230:1101–1107

    Google Scholar 

  37. Spector R (1977) Vitamin homeostasis in the central nervous system. N Engl J Med 296:1393–1398

    Google Scholar 

  38. Taylor AN (1977) Chick brain calcium binding protein: response to cholecalciferol and some developmental aspects. J Nutr 107:480–486

    Google Scholar 

  39. Ziegler R, Minne H, Raue F, Paar G, Delling G (1975) Beobachtungen zur Vitamin-D- und Dihydrotachysterin-Vergiftung. Dtsch Med Wochenschr 100:415–423

    Google Scholar 

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Authors and Affiliations

  1. Zentrum für Kinderheilkunde und Abteilung für Neurochirurgie, Universität Ulm, Germany

    H. -P. Richter & G. Antoniadis

Authors
  1. S. Balabanova
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  2. H. -P. Richter
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  3. G. Antoniadis
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  4. J. Homoki
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  5. N. Kremmer
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  6. J. Hanle
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  7. W. M. Teller
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Balabanova, S., Richter, H.P., Antoniadis, G. et al. 25-hydroxyvitamin D, 24, 25-dihydroxyvitamin D and 1,25-dihydroxyvitamin D in human cerebrospinal fluid. Klin Wochenschr 62, 1086–1090 (1984). https://doi.org/10.1007/BF01711378

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  • DOI: https://doi.org/10.1007/BF01711378

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