Abstract
To determine whether the nasopharynx of children is colonized by a single or multiple strains of nontypeableHaemophilus influenzae, cultures were obtained from six nasopharyngeal sites in five children. For each child, all isolates yielded identical polymerase chain reaction fingerprints. The results indicate that these children were colonized in the nasopharynx with a single strain of nontypeableHaemophilus influenzae at one time.
Similar content being viewed by others
References
Klein JO: Otitis media. Clinical Infectious Diseases 1994, 19: 823–833.
Murphy TF, Bernstein JM, Dryja DD, Campagnari AA, Apicella MA: Outer membrane protein and lipooligosaccharide analysis of paired nasopharyngeal and middle ear isolates in otitis media due to nontypeableHaemophilus influenzae: pathogenetic and epidemiological observations. Journal of Infectious Diseases 1987, 156: 723–731.
Prellner K, Christensen P, Hovelius B, Rosen C: Nasopharyngeal carriage of bacteria in otitis-prone and nonotitis-prone children in day-care centres. Acta Otolaryngologica 1984, 98: 343–350.
Aniansson G, Alm B, Andersson B, Larsson P, Nylen O, Peterson H, Rigner P, Svanborg M, Svanborg C: Nasopharyngeal colonization during the first year of life. Journal of Infectious Diseases 1992, 165, Supplement 1: 38–42.
Harabuchi Y, Faden H, Yamanaka N, Duffy L, Wolf J, Krystofik D: Nasopharyngeai colonization with nontypeableHaemophilus influenzae and recurrent otitis media. Journal of Infectious Diseases 1994, 170: 862–866.
Faden H, Duffy L, Williams A, Krystofik DA, Wolf J: Epidemiology of nasopharyngeai colonization with non-typeableHaemophilus influenzae in the first 2 years of life. Journal of Infectious Diseases 1995, 172: 132–135.
van Belkum A, Duim B, Regelink A, Moller L, Quint W, van Alphen L: Genomic DNA fingerprinting of clinicalHaemophilus influenzae isolates by polymerase chain reaction amplification: comparison with major outermembrane protein and restriction fragment length polymorphism. Journal of Medical Microbiology 1994, 41: 63–68.
Jordens JZ, Leaves NI, Anderson EC, Slack MPE: Polymerase chain reaction-based strain characterization of noncapsulateHaemophilus influenzae. Journal of Clinical Microbiology 1993, 31: 2981–2987.
Versalovic J, Koeuth T, Lupski JR: Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Research 1991, 19: 6823–6831.
Leach AJ, Boswell JB, Asche V, Nienhuys TG, Mathews JD: Bacterial colonization of the nasopharynx predicts very early onset and persistence of otitis media in Australian Aboriginal infants. Pediatric Infectious Diseases Journal 1994, 13: 983–989.
Forsgren J, Samuelson A, Ahlin A, Jonasson J, Rynnel-Dagoo B, Lindberg A:Haemophilus influenzae resides and multiplies intracellularly in human adenoid tissue as demonstrated by in situ hybridization and bacterial viability assay. Infection and Immunity 1994, 62: 673–679.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bernstein, J.M., Dryja, D., Yuskiw, N. et al. Analysis of isolates recovered from multiple sites of the nasopharynx of children colonized by nontypeableHaemophilus influenzae . Eur. J. Clin. Microbiol. Infect. Dis. 16, 750–753 (1997). https://doi.org/10.1007/BF01709258
Issue Date:
DOI: https://doi.org/10.1007/BF01709258