Summary
By means of restriction endonuclease digests and DNA/hybridisation studies we analysed ten representative methicillin-resistantStaphylococcus aureus strains of our collection for plasmid similarities and plasmid associated resistance determinants. We found that strains isolated at our laboratory contained identical or at least most similar plasmids. Isolates from another geographical origin showed different plasmid patterns. We found resistance determinants for gentamicin to be chromosomally encoded, whereas resistance to heavy metal ions and chloramphenicol was always plasmid associated. Resistance to trimethoprim, tetracycline and erythromycin was usually chromosomally mediated but could also reside on a plasmid. Our results indicate that methicillin-resistant strains from our collection may have a common origin. The clinical relevance of these results is discussed.
Zusammenfassung
Wir untersuchten zehn repräsentativeStaphylococcus aureus-Stämme unserer Sammlung mit Hilfe von Restriktionsanalysen und Hybridisierungsstudien auf Ähnlichkeiten in ihrem Plasmid-Gehalt sowie auf Plasmid-kodierte Resistenzen. Hamburger Isolate enthielten dabei sehr ähnliche, wenn nicht gleiche Plasmide. Stämme aus einem anderen Gebiet der Bundesrepublik zeigten dagegen ein stark abweichendes Plasmid-Muster. Die Gentamicinresistenz fanden wir chromosomal-kodiert, während Resistenzen gegenüber Schwermetallionen und Chloramphenicol immer Plasmid-assoziiert waren. Resistenzen gegenüber Trimethoprim, Tetrazyklin und Erythromycin waren normalerweise chromosomalkodiert, konnten aber auch auf Plasmiden gefunden werden. Unsere Ergebnisse legen einen gemeinsamen Ursprung Hamburger Stämme nah. Die klinische Bedeutung dieser Ergebnisse wird diskutiert.
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Gatermann, S. Plasmid fingerprinting of methicillin-resistant staphylococcus aureus strains isolated in Hamburg. Infection 15, 459–462 (1987). https://doi.org/10.1007/BF01647236
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DOI: https://doi.org/10.1007/BF01647236