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Chinese hamster × mouse hybrid cells segregating mouse chromosomes and isozymes

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Somatic Cell Genetics

Abstract

Hybrid cells are readily formed by fusing clonal Chinese hamster cells to fresh, noncultured, adult mouse spleen cells followed by isolation in selective medium. The vast majority of such hybrids retain Chinese hamster chromosomes and isozymes while segregating mouse chromosomes and isozymes. The growth, plating efficiency, ease of karyology, and rapid segregation of mouse markers allows linkage tests in primary clones. Analysis of 13 isozymes showed 12 to be asyntenic and one pair (PGD-PGM2) to be syntenic. This system will allow extensive somatic cell hybrid gene mapping in the mouse and permit a comparison of human and mouse linkage relationships.

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Authors and Affiliations

  1. Laboratory of Biochemical Genetics, National Heart and Lung Institute, Bethesda, Maryland

    John D. Minna, Thomas H. Marshall & Patricia V. Shaffer-Berman

  2. NCI-VA Medical Oncology Branch, National Institutes of Health, Washington, D.C.

    John D. Minna, Thomas H. Marshall & Patricia V. Shaffer-Berman

Authors
  1. John D. Minna
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  2. Thomas H. Marshall
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  3. Patricia V. Shaffer-Berman
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Minna, J.D., Marshall, T.H. & Shaffer-Berman, P.V. Chinese hamster × mouse hybrid cells segregating mouse chromosomes and isozymes. Somat Cell Mol Genet 1, 355–369 (1975). https://doi.org/10.1007/BF01538667

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  • DOI: https://doi.org/10.1007/BF01538667

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