Summary
Virus and cell DNA were separated by isopycnic centrifugation after subjecting a mixture to denaturation and reannealing. Virus DNA renatured, while cell DNA retained the density of single-stranded material. This process magnified the originally very small density difference between the two DNA's. The method's usefulness was demonstrated with mixtures of pure virus DNA and pure cell DNA, and was applied to the study of DNA synthesis in vaccinia infected cells.
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References
Sheinin, R.: Virology28, 621 (1966).
Green, M.: Cold Spr. Harb. Symp. quant. Biol.27, 219 (1962).
Joklik, W. K.: J. molec. Biol.5, 265 (1962).
Schildkraut, C. L., J. Marmur, andP. Doty: J. molec. Biol.4, 430 (1962).
Cairns, H. J. F.: Virology11, 603 (1960).
Dounce, A. L., R. F. Witter, K. J. Monty, andS. Pate: J. biophys. biochem. Cytol.1, 139 (1955).
Joklik, W. K., andY. Becker: J. molec. Biol.10, 452 (1964).
Marmur, J., andP. Doty: J. molec. Biol.3, 595 (1961).
Schildkraut, C. L., J. Marmur, andP. Doty: J. molec. Biol.3, 595 (1961).
Subirana, J. A.: Biopolymers4, 189 (1966).
Britten, R. C., andM. J. Waring: In: Carnegie Inst. of Washington, Year Book 64, pp. 316–333 (1965).
Marmur, J.: J. molec. Biol.3, 208 (1961).
Dawid, I. B.: J. molec. Biol.12, 581 (1965).
Hearst, J. E., andJ. Vinograd: Fortschr. Chem. organ. Naturstoffe20, 372 (1962).
Szybalski, W., R. L. Erikson, G. A. Gentry, L. G. Gafford, andC. C. Randall: Virology19, 586 (1963).
Hershey, A. D., E. Burgi, andC. I. Davern: Biochem. biophys. Res. Commun.18, 675 (1965).
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Supported by Stiftung Volkswagenwerk and Deutsche Forschungsgemeinschaft (We 165/3).
On leave of absence from the Carnegie Institute of Washington.
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Jungwirth, C., Dawid, I.B. Vaccinia DNA: Separation of viral from host cell DNA. Archiv f Virusforschung 20, 464–468 (1967). https://doi.org/10.1007/BF01275228
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DOI: https://doi.org/10.1007/BF01275228