Abstract
The circular and linear forms of viroid RNAs can be separated by two-dimensional polyacrylamide gel electrophoresis (PAGE) based on the selective delay in mobility that circular RNAs experience under denaturing conditions. First PAGE separates RNA preparations from viroid-infected plants, and the whole lane from this first gel is next perpendicularly loaded on top of a second gel. Separation continues under new conditions that differ in the degree of denaturation from the first. The result is a two-dimensional separation of the RNAs in which circular and linear molecules are distributed in two parallel diagonals.
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Acknowledgments
I thank Verónica Aragonés for excellent technical assistance. This work was supported by grants BIO2017-83184-R and BIO2017-91865-EXP from Ministerio de Cienciae, Innovación (Spain), co-financed by European Regional Development Fund (European Commission).
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Daròs, JA. (2022). Two-Dimensional Polyacrylamide Gel Electrophoresis Analysis of Viroid RNAs. In: Rao, A.L.N., Lavagi-Craddock, I., Vidalakis, G. (eds) Viroids. Methods in Molecular Biology, vol 2316. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1464-8_8
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DOI: https://doi.org/10.1007/978-1-0716-1464-8_8
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