Abstract
A summary of the ion types observed in the Fast Atom Bombardment Mass Spectrometry (FAB-MS) and collision induced decomposition (CID) MS/MS spectra of glycoconjugates (glycosphingolipids, glycopeptides, glycosides and carbohydrates) is presented. The variety of product ion types that arise by cleavages within the carbohydrate moieties has prompted us to introduce a systematic nomenclature to designate these ions. The proposed nomenclature has been developed primarily for FAB-MS, but can be used as well for other ionization techniques [field desorption (FD), direct chemical ionization (DCI), laser desorption/Fourier transform (LD/FT), etc.], and is applicable to spectra recorded in either the positive or negative ion mode during both MS and MS/MS experiments.
Ai, Bi and Ci labels are used to designate fragments containing a terminal (nonreducing end) sugar unit, whereas Xj, Yj and Zj represent ions still containing the aglycone (or the reducing sugar unit). Subscripts indicate the position relative to the termini analogous to the system used in peptides, and superscripts indicate cleavages within carbohydrate rings.
FAB-MS/MS spectra of a native glycosphingolipid and glycopeptide, and a permethylated ganglioside are shown as illustrations.
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Abbreviations
- FAB-MS:
-
fast atom bombardment mass spectrometry
- CID:
-
collision induced decomposition
- DCI:
-
direct chemical ionisation
- FD:
-
field desorotion
- LD/FT:
-
laser desorption/Fourier transform
- EI:
-
electron impact
- Gm1-ganglioside:
-
II3NeuAc-GgOse4Cer
- Fuc-GM1:
-
IV2Fuc,II3NeuAc-GgOse4Cer
- hex:
-
hexose
- hexNAc:
-
N-acetylhexosamine
References
Barber M, Bordoli RS, Sedgwick RD, Vickerman JC (1982) J Chem Soc Faraday Trans 1 78:1291-96.
Egge H, Peter-Katalinic J (1987) Mass Spectrom Rev 6:331–93.
Biemann K (1986) Anal Chem 58:1288A-1300A.
Domon B, Costello CE (1988) Biochemistry 27:1534–43.
Carr SA, Reinhold VN, Green BN, Hass JR (1985) Biomed Mass Spectrom 12:288–95.
Crow FW, Tomer KB, Looker JH, Gross ML, (1986) Anal Biochem 155:286–307.
Costello CE, Domon B, Zeng C-H, Qian F, Du S, Li M (1987) Proc Ann Conf Mass Spectrom Allied Topics 35:1–2.
Fraisse D, Tabet JC, Becchi M, Raynaud J (1986) Biomed Mass Spectrom 13:1–14.
Dell A (1987) Adv Carbohydt Chem Biochem 45:19–72.
Coates ML, Wilkins CL (1987) Anal Chem 59:197–200.
Kochetkov NK, Chizhov OS (1966) Adv Carbohydr Chem Biochem 21:39–93.
Lönngren J, Svensson S (1974) Adv Carbohydr Chem Biochem 29:41–106.
Roepstorff P, Fohlman J (1984) Biomed Mass Spectrom 11:601.
Biemann K, Martin SA (1987) Mass Spectrom Rev 6:1–76.
Johnson RS, Biemann K (1987) Biochemistry 26:1209–14.
Spik G, Bayard B, Fournet B, Strecker G, Bouquelet S, Montreuil J (1975) FEBS Lett 50:296–99.
Ciucanu J, Kerek K (1984) Carbohydr Res 131:209–17.
Larson G, Karlsson H, Hansson GC, Pimlott W (1987) Carbohydr Res 161:281–90:
Sato K, Asada T, Kunihiro F, Kammei Y, Kubota E, Costello CE, Martin SA, Scoble HA, Biemann K (1987) Anal Chem 59:1652–59.
Prome JC, Aurelle H, Prome D, Savagnac A (1987) Org Mass Spectrom 22:6–12.
Gunnarsson A (1987) Glycoconjugate J 4:239–45.
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Domon, B., Costello, C.E. A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates. Glycoconjugate J 5, 397–409 (1988). https://doi.org/10.1007/BF01049915
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DOI: https://doi.org/10.1007/BF01049915