Abstract
Conditions for the isolation of intact poly(A)+mRNP from cryptobiotic gastrulae ofA. salina are described. In the presence of Mg2+ ions nucleolytic cleavage occurs in vitro in the vicinity of the 3′-poly(A) segment of mRNP during the isolation procedure. The resulting two parts of poly(A)+mRNP complex are separated by thermal elution from oligo(dT)-cellulose affinity column. Analysis by SDS-gel electrophoresis of protein components associated with intact poly(A)+mRNP has revealed the existence of 20–30 S RNP complex containing five major proteins with Mr 68,000, 53,000, 50,000, 45,000 and 38,000, respectively, but completely lacking the poly(A)-specific Mr 76,000 protein.
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References
GrosfeldH. and LittauerU.Z., Biochem. Biophys. Res. Commun. 67, 176–181 (1975).
SlegersH., MettrieR. and KondoM., FEBS Letters 80, 390–394 (1977).
De Herdt, E., Slegers, H. and Kondo, M., (submitted for publication).
SlegersH. and KondoM., Nucleic Acids Res. 4, 625–639 (1977).
SimonsJ., DeHerdtE., KondoM. and SlegersH., FEBS Letters 91, 53–56 (1978).
BlanchardJ.M., BrissacC. and JeanteurPh., Proc. Acad. Nat. Sci. U.S.A. 71, 1882–1886 (1974).
VanVenrooijW.J., vanEekelenC.A.G., JansenR.T.P., and PrincenJ.M.G., Nature 270, 189–191 (1977).
VincentA., CivelliO., BuriJ.-F. and ScherrerK., FEBS Letters 77, 281–286 (1977).
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Slegers, H., De Herdt, E. & Kondo, M. Characterization of protein components of poly(A)-containing messenger ribonucleoproteins from cryptobiotic gastrulae of Artemia salina. Mol Biol Rep 5, 65–69 (1979). https://doi.org/10.1007/BF00777490
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DOI: https://doi.org/10.1007/BF00777490