Abstract
The induction by d,l-nicotine of the enantiozymes 6-hydroxy-L-nicotine oxidase and 6-hydroxy-D-nicotine oxidase in Archrobacter oxidans was differently affected by the inhibitors of Escherichia coli gyrase, novobiocin and nalidixic acid. These compounds inhibited 6-hydroxy-L-nicotine oxidase induction slightly, but led to an increase in the level of 6-hydroxy-D-nicotine oxidase activity. Furthermore, the specific repression by glucose of 6-hydroxy-D-nicotine oxidase synthesis was not abolished by the addition of cAMP but by that of novobiocin.
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Abbreviations
- 6-HDNO:
-
6-hydroxy-D-nicotine oxidase
- 6-HLNO:
-
6-hydroxy-L-nicotine oxidase
- cAMP:
-
cyclic 3′,5′-adenosine monophosphate
- Enzymes:
-
Adenylate cyclase
- ATP:
-
pyrophosphate-lyase (cyclizing) (EC 4.6.1.1)
- cAMP-phosphodiesterase:
-
3′:5′-cyclic-nucleotide 5′-nucleotido-hydrolase (EC 3.1.4.17)
- DNA gyrase:
-
DNA topoisomerase II (EC 5.99)
- DNA polymerase:
-
deoxynucleosidetriphosphate: DNA desoxynucleotidyl-transferase (EC 2.7.7.7)
- 6-hydroxy-L-nicotine oxidase:
-
6-hydroxy-L-nicotine: oxygen oxidoreductase (EC 1.5.3.5)
- 6-hydroxy-D-nicotine oxidase:
-
6-hydroxy-D-nicotine: oxygen oxidoreductase (EC 1.5.3.6)
- β-lactamase:
-
penicillin amido-β-lactamhydrolase (EC 3.5.2.6)
- nicotine dehydrogenase:
-
nicotine: (acceptor)6-oxidoreductase (hydroxylating) (EC 1.5.99.4)
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Brandsch, R., Decker, K. The effect of gyrase inhibitors and cyclic AMP on induction and glucose repression of the 6-hydroxy-nicotine oxidases in Arthrobacter oxidans . Arch. Microbiol. 133, 274–277 (1982). https://doi.org/10.1007/BF00521289
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DOI: https://doi.org/10.1007/BF00521289