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Consecutive demonstration of catecholamines and dopamine-β-hydroxylase within the same specimen

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Summary

A combined method was developed for characterization and differentiation of catecholamines in neuron populations containing more than one catecholamine, e.g. dopamine and norepinephrine. Its application to small intensely fluorescent (SIF-) cell clusters in sympathetic ganglia allows the successive demonstration of glyoxylic acid-induced catecholamine fluorescence and dopamine-β-hydroxylase by indirect immunofluorescence within the same tissue section. Applying this technique to an example, two types of SIF-cells were demonstrated in the guinea pig superior cervical ganglion.

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Authors and Affiliations

  1. Anatomisches Institut der Universität, Im Neuenheimer Feld 307, D-6900, Heidelberg, Federal Republic of Germany

    R. König

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  1. R. König
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Supported by the “Deutsche Forschungsgemeinschaft” grant He 919/4

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König, R. Consecutive demonstration of catecholamines and dopamine-β-hydroxylase within the same specimen. Histochemistry 61, 301–305 (1979). https://doi.org/10.1007/BF00508451

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  • DOI: https://doi.org/10.1007/BF00508451

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