Skip to main content
SpringerLink
Log in

Demonstration of intracytoplasmic glycogen of megakaryocytes and blood platelets by means of the periodic acid-thiocarbohydrazide-silver proteinate method

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

The glycogen of megakaryocytes and blood platelets has been investigated in glutaraldehyde and osmium tetroxide fixed tissues by the periodic acid-thiocarbohydrazide-silver proteinate method (PA-TCH-SP). The PA-TCH-SP method involves the staining of intracytoplasmic glycogens more densely than the routine lead citrate method. Glycogen having a mean particle diameter of 21.1 nm has been shown localizing in the matrix of mature megakaryocytes, while that of glycogen in the platelets was 26.2 nm. The staining pattern of the glycogen in blood platelets was classified into three groups according to staining intensity.

It is found that the PA-TCH-SP method is a very suitable one for the demonstration of intracytoplasmic glycogen from the viewpoints of reaction specificity, reproducibility, fineness of reaction products, sufficiency of electron density, and experimental cost. This method is also a very useful one for differentiating intracytoplasmic glycogens and ribosomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson, W.A.: Cytochemistry of sea-urchian gametes. 1. Intramitochondrial localization of glycogen, glucose 6-phosphatase and adenosine triphosphatase in spermatozoa of paracentrotus lividus. J. Ultrastruct. Res. 24, 398–411 (1968)

    Google Scholar 

  • Anderson, W.A., Personne, P.: The localization of glycogen in the spermatozoa of various invertebrate and vertebrate species. J. Cell Biol. 44, 29–51 (1970)

    Google Scholar 

  • Bernhold, C.H.: Ultrastructural appearance of glycogen in the β-neurons in the lumbar spinal ganglion of the frogs. J. Ultrastruct. Res. 14, 254–267 (1966)

    Google Scholar 

  • Bradbury, S., Stoward, P.J.: The specific cytochemical demonstration in the electron microscope of periodate-reactive mucosubstances and polysaccharides containing vic-glycol groups. Histochemie 11, 71–80 (1967)

    Google Scholar 

  • Cawley, J.C., Hayhoe, F.G.J.: The megakaryocyte series. In: ultrastructure of haemic cells: a cytological atlas of normal and leukaemic blood and bone marrow, p. 122–131. London Philadelphia toronto: W.B. Saunders 1973

    Google Scholar 

  • Davis, R.B.: Glycogen distribution in rat blood platelets. Amer. J. Pathol. 72, 241–252 (1973)

    Google Scholar 

  • Drochman, P.: Morphologie du glycogène: Etude au microscope électronique de coloration négative du glycogène particulaire. J. Ultrastruct. Res. 6, 141–163 (1962)

    Google Scholar 

  • Gibb, R.P., Stowell, R.E.: Glycogen in human blood cells. Blood 4, 569–579 (1949)

    Google Scholar 

  • Hanker, J.S., Seaman, A.R., Weiss, L.P., Ueno, H., Bergman, R.A., Seligman, A.M.: Osmiophilic reagents: new cytochemical principle for light and electron microscopy. Science 146, 1039–1043 (1964)

    Google Scholar 

  • Ishikawa, T., Pei, Y.F.: Intramitochondrial glycogen particles in rat retinal receptor cells. J. Cell Biol. 25, 402–407 (1965)

    Google Scholar 

  • Itoga, T., Oka, S., Inoko, K.: Methods of polysaccharide staining by means of PAS including simplified method. (In Japanese). Rinsho Byori Suppl. 13, 55–64 (1967)

    Google Scholar 

  • Jain, N.C.: Glycogen content of leukocytes of some animal species. Acta haematol. (Basel) 41, 249–253 (1969)

    Google Scholar 

  • Lentz, T.L.: Intramitochondrial glycogen granules in digestive cells of hydra. J. Cell Biol. 29, 162–167 (1966)

    Google Scholar 

  • Leske, E., Mayersbach, H. v.: The role of histochemical and biochemical preparation methods for the detection of glycogen. J. Histochem. Cytochem. 17, 527–538 (1969)

    Google Scholar 

  • Luft, J.H.: Improvement in epoxy resin embedding methods. J. Biophys. Biochem. Cytol. 9, 409–414 (1961)

    Google Scholar 

  • Miwa, S.: Staining methods of dehydrogenase, esterase and phosphorylase (In Japanese). Rinsho Byori Suppl. 13, 49–54 (1967)

    Google Scholar 

  • Monneron, A., Bernhard, W.: Action de certaines enzymes sur de tissus inclus en epon. J. Microscopie 5, 697–714 (1966)

    Google Scholar 

  • Murata, F., Hardin, J.H., Spicer, S.S.: Coexistence of acid phosphatase and acid mucosubstance in the nucleoid of human blood platelets granules. Histochemie 35, 319–329 (1973a)

    Google Scholar 

  • Murata, F., Spicer, S.S.: Morphologic and cytochemical studies of rabbit heterophilic leukocytes: evidence for tertiary granules. Lab. Invest. 20, 65–72 (1973b)

    Google Scholar 

  • Murata, F., Nagata, T., Spicer, S.S.: Fine structural localization of arylsulfatase B activity in the rabbit blood platelets. Histochemistry 44, 307–312 (1975)

    Google Scholar 

  • Nawa, T., Nagata, T., Yokota, S., Murata, F.: Omochi, S.: Two new models of freeze-drying apparatus for tissues. Med. J. Schinshu Univ. 14, 1–15 (1969)

    Google Scholar 

  • Oguro, M., Hayami, K.: Procedure of PAS staining of blood smear preparations including Giemsa staining and its clinical significace. (In Japanese). Rinsho Byori Suppl. 13, 7–13 (1965)

    Google Scholar 

  • Quaglino, D., Hayhoe, F.G.J.: Phosphorylase activity in haemic cells. Nature 4832, 929–931 (1962)

    Google Scholar 

  • Rambourg, A.: An improved silver methenamine technique for the detection of periodic acid-reactive complex. J. Histochem. Cytochem. 15, 409–412 (1967)

    Google Scholar 

  • Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–211 (1963)

    Google Scholar 

  • Sabatini, D.D., Bensch, K., Barrnett, R.J.: Cytochemistry and electron microscopy: the preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963)

    Google Scholar 

  • Scott, R.B.: Activation of glycogen phosphorylase in blood platelets. Blood 30, 321–330 (1967)

    Google Scholar 

  • Scott, R.B.: The role of glycogen in blood cells. New Eng. J. Med. 278, 1436–1439 (1968)

    Google Scholar 

  • Stoward, P.J., Bradbury, S.: A note on the p-fluorophenylhydrazine for the cytochemical demonstration of periodate-reactive mucosaccharides and polysaccharides in the electron microscope. Histochemie 15, 93–94 (1968)

    Google Scholar 

  • Takeuchi, T., Kinoshita, K.: Histochemical demonstration of phosphorylase in blood and bone marrow cells. Blood 11, 375–379 (1956)

    Google Scholar 

  • Takeuchi, T.: carbohydrate, homoglycans. (in Japanese). In: New Histochemistry, p. 525–539. Tokyo: Asakura 1975

    Google Scholar 

  • Themann, H.L Zur elektronenmikroskopischen Darstellung von Glycogen mit Best's Carmin. J. Ultrastruct. Res. 4, 401–412 (1960)

    Google Scholar 

  • Thiéry, J.P.: Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J. Microscopie 6, 987–1018 (1967)

    Google Scholar 

  • Wachstein, M.: The distribution of histochemically demonstrable glycogen in human blood and bone marrow cells. Blood 4, 54–59 (1949)

    Google Scholar 

  • Watson, M.L.: Staining of tissue sections for electron microscopy with heavy metals. J. Biophys. Biochem. Cytol. 4, 475–478 (1958)

    Google Scholar 

  • Wislocki, G.B., Rheingold, J.J., Dempsey, E.W.: The occurrence of the periodic acid-Schiff reaction in various normal cells of blood and connective tissue. Blood 4, 562–568 (1949)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, Shinshu University School of Medicine, 390, Matsumoto, Japan

    Fusayoshi Murata, Yuriko Momose & Tetsuji Nagata

Authors
  1. Fusayoshi Murata
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yuriko Momose
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tetsuji Nagata
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Murata, F., Momose, Y. & Nagata, T. Demonstration of intracytoplasmic glycogen of megakaryocytes and blood platelets by means of the periodic acid-thiocarbohydrazide-silver proteinate method. Histochemistry 52, 307–316 (1977). https://doi.org/10.1007/BF00508403

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00508403

Keywords

Search

Navigation