Summary
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1.
The influence of (+)-propranolol, 10−6 to 4×10−5 mol/l, upon the positive inotropic effect of dihydro-ouabain was studied on guinea-pig papillary muscle. At 1 Hz, negative inotropically effective concentrations of (+)-propranolol decreased the inotropic effectiveness of the glycoside.
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2.
For equivalent diminution of the basal force of contraction, either by reduction of stimulation frequency or by increasing concentrations of (+)-propranolol, a similar decrease in the inotropic effectiveness of dihydro-ouabain was observed. In contrast, reduction of [Ca2+]0 had almost no effect on the effectiveness of the glycoside.
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3.
The inhibitory effect of (+)-propranolol on the inotropic effectiveness of dihydro-ouabain declined with reduction of stimulation frequency. At any stimulation frequency tested, the inhibitory effect of (+)-propranolol was proportional to the effect of a reduction of that particular frequency to the rested-state condition in the absence of (+)-propranolol.
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4.
The results are consistent with the hypothesis that the inhibition of the excitation-coupled sodium influx by (+)-propranolol is the cause of its inhibiting effect on the effectiveness of dihydro-ouabain.
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Ebner, F., Reiter, M. The influence of (+)-propranolol on the inotropic effect of dihydro-ouabain in relation to stimulation frequency. Naunyn-Schmiedeberg's Arch. Pharmacol. 307, 105–113 (1979). https://doi.org/10.1007/BF00498451
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DOI: https://doi.org/10.1007/BF00498451