Summary
Methodological studies on the histochemical technique for the demonstration of G6Pase activity showed that the occurrence of common artifacts: morphological destruction, extracellular precipitation of reaction product and nuclear staining are dependent on the concentration of lead nitrate, buffer and substrate. By studying the effects of systematic variation of the incubation media on the histochemical reaction optimal concentrations of either of these components were determined. An improved medium containing 3.6 mM lead nitrate, 40 mM tris-maleate buffer, pH 6.5, 10 mM G6P and 300 mM sucrose was used for the study of G6Pase distribution patterns in liver acini of juvenile and adult rats of both sexes and in those of starved adult female rats. The results obtained indicate sex dependent differences in the functional organization of the liver acinus and furthermore demonstrate the rapid functional adaptability of liver parenchyma to changes of the nutritional situation.
Similar content being viewed by others
References
Chiquoine, A.D.: The distribution of glucose-6-phosphatase in the liver and kidney of the mouse. J. Histochem. Cytochem. 1, 429–439 (1953)
Chiquoine, A.D.: Further studies on the histochemistry of glucose-6-phosphatase. J. Histochem. Cytochem. 3, 471–478 (1955)
Glenner, G.G.: Evaluation of the specificity of enzyme histochemical reactions. J. Histochem. Cytochem. 16, 519–529 (1968)
Holt, S.J.: Factors governing the validity of staining methods for enzymes, and their bearing upon the Gomori acid phosphatase technique. Exp. Cell. Res. Suppl. 7, 1–27 (1959)
Jacobsen, N.O., Jørgensen, P.L.: A quantitative biochemical and histochemical study of the lead method for localization of adenosine triphosphate-hydrolyzing enzymes. J. Histochem. Cytochem. 17, 443–453 (1969)
Katz, N., Teutsch, H.F., Sasse, D., Jungermann, K.: Heterogeneous distribution of glucose-6-phosphatase in microdissected periportal and perivenous rat liver tissue. FEBS Lett. 76, 226–230 (1977)
Koudstaal, J., Hardonk, M.J.: Histochemical demonstration of enzymes in rat liver during post-natal development. Enzymes related to NADPH-dependent hydroxylating systems and to sexdifference. Histochemie 23, 71–81 (1970)
Leskes, A., Siekevitz, P., Palade, G.E.: Differentiation of endoplasmic reticulum in hepatocytes. I. Glucose-6-phosphatase distribution in situ. J. Cell Biol. 49, 264–287 (1971)
Ménard, D., Penasse, W., Drochmans, P., Hugon, J.S.: Glucose-6-phosphatase heterogeneity within the hepatic lobule of the phenobarbital-treated rat. Histochemistry 38, 229–239 (1974)
Novikoff, A.: Cell heterogeneity within the hepatic lobule of the rat. (Staining reactions). J. Histochem. Cytochem. 7, 240–244 (1959)
Pearse, A.G.E.: Histochemistry. Theoretical and applied. 3rd ed. Vol. I. London: J. & A. Churchill 1968
Rappaport, A.M.: The microcirculatory acinar concept of normal and pathological hepatic structure. Beitr. Pathol. 157, 215–243 (1976)
Rosenthal, A.S., Moses, H.L., Tice, L., Genote, CH.E.: Lead ion and phosphatase histochemistry. III. The effects of lead and adenosine triphosphate concentration on the incorporation of phosphate into fixed tissue. J. Histochem. Cytochem. 17, 608–612 (1969)
Sasse, D., Katz, N., Jungermann, K.: Functional heterogeneity of rat liver parenchyma and of isolated hepatocytes. FEBS Lett. 57, 83–88 (1975)
Sasse, D., Teutsch, H.F., Katz, N., Jungermann, K.: Arbeitsteilung und adaptive Gesamtleistung des Leberparenchyms. Histochemische und biochemische Untersuchungen zum Kohlenhydratstoffwechsel. Verh. Anat. Ges. 71, 873–877 (1977)
Teutsch, H.F., Sasse, D., Katz, N., Jungermann, K.: Zur qualitativen und quantitativen Chemomorphologie des Leberparenchyms (G6Pase). Verh. Anat. Ges. 72, (in press)
Tice, L., Barrnett, R.: The fine structural localization of glucose-6-phosphatase in rat liver. J. Histochem. Cytochem. 10, 754–762 (1962)
Tice, L.: Lead-adenosine triphosphate complexes in adenosine triphosphatase histochemistry. J. Histochem. Cytochem. 17, 85–94 (1968)
Wachstein, M., Meisel, E.: On the histochemical demonstration of glucose-6-phosphatase. J. Histochem. Cytochem. 4, 592 (1956)
Author information
Authors and Affiliations
Additional information
Supported by a grant of the Deutsche Forschungsgemeinschaft
Rights and permissions
About this article
Cite this article
Teutsch, H.F. Improved method for the histochemical demonstration of glucose-6-phosphatase activity. Histochemistry 57, 107–117 (1978). https://doi.org/10.1007/BF00496675
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00496675