Summary
In tissue of rats with disseminated intravascular coagulation, fibrin microthrombi can be sensitively detected by immunohistochemical methods, using antisera against rat fibrinogen or fibrin monomer. An indirect immunoperoxidase procedure on paraplast-embedded sections yields best results with regard to the morphology of the thrombi and their localization in the tissue.
Only fibrillar immunoreactive material, oriented lengthwise in the vessels, should be regarded as microthrombi formed in vivo.
Similar content being viewed by others
References
Arias, F., Mancilla-Jimenez, R.: Hepatic fibrinogen deposits in pre-eclampsia. N. Engl. J. Med. 295, 578–582 (1976)
Beneke, G.: Demonstration of plasma proteins in microscopic sections with emphasis on the identification of fibrin. In: Thrombosis and bleeding disorders (ed. N.U. Bang, F.K. Beller, E. Deutsch, E.F. Mammen), p. 524–533. Stuttgart: Georg Thieme 1971
Bleyl, U.: Morphologic diagnosis of disseminated intravascular coagulation: histologic, histochemical and electronmicroscopic studies. Semin. Thromb. Hemostasis 3, 247–267 (1977)
Bleyl, U., Sebening, H., Kuhn, W.: Morphologischer Nachweis von Fibrinomeren im histologischen Schnitt. Thromb. Diath. Haemorrh. 22, 68–86 (1969)
Boyd, J.F.: Disseminated fibrin thrombo-embolism among stillbirths and neonatal deaths. J. Pathol. Bact. 90, 53–63 (1965)
Davidson, A.M., Thomson, D., Macdonald, M.K., Rae, J.K., Uttley, W.S., Clarkson, A.R.: Identification of intrarenal fibrin deposition. J. clin. Pathol. 26, 102–112 (1973)
Emeis, J.J., Lindeman, J.: Rat liver macrophages will not phagocytose fibrin during disseminated intravascular coagulation. Haemostasis 5, 193–210 (1976)
Godal, H.C., Abildgaard, U.: Gelation of soluble fibrin in plasma by ethanol. Scand. J. Haemat. 3, 342–350 (1966)
Grabar, P.: The immuno-electrophoretic method of analysis. In: Immunoelectrophoretic analysis (ed. P. Grabar, P. Burtin), p. 3–29. Amsterdam: Elsevier 1964
Graham, R.C., Karnovsky, M.J.: The early stages of absorbtion of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291–302 (1966)
Haverkate, F., Timan, G.: Preparation of highly purified bovine fibrin plates. In: Progress in chemical fibrinolysis and thrombolysis (ed. J.F. Davidson, M.M. Samama, P.C. Desnoyers), p. 67–71. New York: Raven Press 1976
MacIver, A.G.: A comparison of tinctorial and immunohistological methods for the detection of fibrinoid change and fibrin deposition in the kidney. Histochem. J. 4, 169–176 (1972)
Margaretten, W., Csavossy, I., McKay, D.G.: An electron microscopic study of thrombin-induced disseminated intravascular coagulation. Blood 29, 269–275 (1967)
Mueller-Berghaus, G.: Pathophysiology of generalized intravascular coagulation. Semin. Thromb. and Hemostasis 3, 209–246 (1977)
Mueller-Berghaus, G., Mahn, I., Köveker, G., Maul, F.D.: In vivo behavior of homologous urea-soluble 131I-fibrin and 125I-fibrinogen in rabbits. Brit. J. Haematol. 33, 61–79 (1976)
Nieuwenhuizen, W., Emeis, J.J., Sabben, C.M. van: Localization of lipase-like immunoreactivity in porcine adipose, aortic and myocardial tissue. Atherosclerosis 27, 97–106 (1977)
Nordstoga, K.: Disseminated intravascular coagulation: a pathologist's view. Thromb. Haemostas. 37, 180 (1977)
Orfila, C., Duffaut, M., Mignon-Conte, M., Rumeau, J.-L., Bugat, R., Suc J.-M.: Localisation en immunofluorescence directe dans le tissu hépatique de l'AgHBs, des immunoglobulines, de la fibrine et de la fraction C3 du complément. Pathol. Biol. 24, 313–318 (1976)
Ouchterlony O.: Handbook of immunodiffusion and immunoelectrophoresis, p. 25. London: Ann Arbor-Humphrey Science 1968
Robboy S.J., Colman R.W., Minna J.D.: Pathology of disseminated intravascular coagulation. Human Pathol. 3, 327–343 (1972)
Ruijven-Vermeer, I.A.M. van, Nieuwenhuizen, W.: Purification of rat fibrinogen and its constituent chains. Biochem. J. 169, 653–658 (1978)
Streefkerk, J.G.: Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol. J. Histochem. Cytochem. 20, 829–831 (1972)
Taylor, C.R., Burns, J.: The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody. J. clin. Pathol. 27, 14–20 (1974)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Craane, H., Emeis, J.J., Lindeman, J. et al. Immunoenzymehistochemical detection of fibrin microthrombi during disseminated intravascular coagulation in rats. Histochemistry 57, 97–105 (1978). https://doi.org/10.1007/BF00496674
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00496674