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Substance P immunoreactivity in the rat mammary nipple and the effects of capsaicin treatment on lactation

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Summary

  1. 1.

    Tissue concentrations of substance P immunoreactivity (SP-I) were measured in rat mammary nipples and were significantly greater than in ventral abdominal skin in nonpregnant and pregnant rats. In contrast, the concentration of nipple SP-I was lower than that of skin in twelve day lactating animals.

  2. 2.

    The mean total SP-I content of the pooled twelve nipples from each rat was not significantly different in nonpregnant, pregnant or lactating rats. However, the mean weight of the pooled twelve nipples from each rat was significantly higher in the lactating rats than in pregnant rats.

  3. 3.

    Immunohistochemistry revealed SP-I nerve trunks and single fibers throughout the nipples of lactating rats. Nerve fibers were observed among smooth muscle and along blood vessels throughout the dermis and in association with epidermal structures. Some SP-I fibers were also observed in association with the main lactiferous duct and mammary gland secretory parenchyma.

  4. 4.

    Radioimmunoassay and immunohistochemistry of nipples from lacting rats treated with capsaicin as neonates revealed a marked depletion of SP-I.

  5. 5.

    Rats treated with capsaicin as neonates had a normal gestation period and produced litters of normal size and birth weight. However, the litters of these lactating rats grew at a significantly slower rate than litters from controls.

  6. 6.

    The quantity of milk obtained from capsaicintreated lactating dams, following a one hour suckling period on the twelfth day of lactation, was significantly less than that obtained by litters of control dams.

  7. 7.

    It is concluded that capsaicin-sensitive primary sensory nerves of the mammary nipple play a role in the afferent limb of the suckling reflex. One transmitter candidate for these nerves is substance P.

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References

  • Amenomori CL, Chen CL, Meites J (1970) Serum prolactin levels in rats during different reproductive states. Endocrinology 86:506–510

    Google Scholar 

  • Brodin E, Nilsson G (1981) Concentration of substance P-like immunoreactivity (SPLI) in tissue of dog, rat and mouse. Acta Physiol Scand 112:305–312

    Google Scholar 

  • Cauna M (1969) The fine morphology of the sensory receptor organs in the auricle of the rat. J Comp Neurol 136:81–98

    Google Scholar 

  • Chang M, Leeman S (1970) Isolation of a sialogogic peptide from bovine hypothalamic tissue and its characterization as substance P. J Biol Chem 245:4784–4790

    Google Scholar 

  • Costa M, Buffa R, Furness J, Solcia E (1980) Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparations. Histochemistry 65: 157–165

    Google Scholar 

  • Cotton J, Traurig H, Papka R (1983) Substance P and vasoactive intestinal polypeptide-immunoreactive nerves in the female reproductive system. Anat Rec 205:38A-39A

    Google Scholar 

  • Cowie AT, Tindall JS (1971) The neuroendocrine regulation of mammary function. In: The Physiology of Lactation. Williams and Wilkins. Baltimore, pp 223–282

    Google Scholar 

  • Cowie AT, Forsyth IA, Hart IC (1980) Lactation. In: Cowie AT, Forsyth IA, Hart IC (eds) Hormonal control of Lactation. Springer, Berlin Heidelberg New York, pp 146–210

    Google Scholar 

  • Cuello AC, Del Fiacco M, Paxinos G (1978) The central and peripheral ends of the substance P-containing sensory neurones in the rat trigeminal system. Brain Res 152: 499–509

    Google Scholar 

  • Edwardson JA, Eayrs JT (1967) Neural factors in the maintenance of lactation in the rat. J Endocrinol 38:51–59

    Google Scholar 

  • Findley ALR (1966) Sensory discharges from lactating mammary glands. Nature 211:1183–1184

    Google Scholar 

  • Findley ALR (1968) The effect of teat anesthesia on the milkejection reflex in the rabbit. J Endocrinol 40:127–128

    Google Scholar 

  • Fitzgerald M (1983) Capsaicin and sensory neurons—a review. Pain 15:109–130

    Google Scholar 

  • Floor E, Leeman SE (1980) Substance P sulfoxide: Separation from substance P by high-pressure liquid chromatography, biological and immunological activities, and chemical reactions. Anal Biochem 101:498–503

    Google Scholar 

  • Freeman ME, Sterman JR (1978) Ovarian steroid modulation of prolactin surges in cervically stimulated rats. Endocrinology 102:1915–1920

    Google Scholar 

  • Freeman ME, Smith MS, Nazian SJ, Neill JD (1974) Ovarian and hypothalamic control of the daily surges of prolactin secretion during pseudopregnancy in the rat. Endocrinology 94: 875–882

    Google Scholar 

  • Gamse R (1982) Capsaicin and nociception in the rat and mouse. Possible role of substance P. Naunyn-Schmiedeberg's Arch Pharmacol 320:205–216

    Google Scholar 

  • Gamse R, Lembeck F, Cuello A (1979) Substance P in the vagus nerve-immunochemical and immunohistochemical evidence for axoplasmic transport. Naunyn-Schmiedeberg's Arch Pharmacol 306:37–44

    Google Scholar 

  • Gamse R, Holzer P, Lembeck F (1980) Decrease of substance P in primary afferent neurones and impairment of neurogenic plasma extravasation by capsaicin. Br J Pharmacol 68: 207–213

    Google Scholar 

  • Gamse R, Lackner D, Gamse G, Leeman S (1981) Effect of capsaicin pretreatment on capsaicin-evoked release of immunoreactive somatostatin and substance P from primary sensory neurons. Naunyn-Schmiedeberg's Arch Pharmacol 316:38–41

    Google Scholar 

  • Gram BR, Ottesen B (1982) Increased myometrial blood flow evoked by substance P. Pflügers Arch 395:347–350

    Google Scholar 

  • Grosvenor CE (1965) Evidence that extroceptive stimuli can release prolactin from the pituitary gland of the lactating rat. Endocrinology 76:340–342

    Google Scholar 

  • Grosvenor CE, Mena F (1967) Effect of auditory, olfactory and optic stimuli upon milk ejection and suckling-induced release of prolactin in lactating rats. Endocrinology 80:840–846

    Google Scholar 

  • Grosvenor CE, Findley ALR (1968) Effect of denervation on fluid flow into the rat mammary gland. Am J Physiol 214:820–824

    Google Scholar 

  • Grosvenor CE, Mena F, Schaefgen DA (1967) The effect of nonsuckling interval and duration of suckling on the suckling-induced fall in pituitary prolactin concentration in the rat. Endocrinology 81:449–453

    Google Scholar 

  • Gu J, Huang W, Islam K, McGregor G, Terenghi G, Morrison J, Bloom S, Polak J (1983) Substance P-containing nerves in the mammalian genitalia. In: Skrabanek P and D Powell (eds) Substance P-Dublin 1983. Boole Press Limited, Dublin, pp 263–264

    Google Scholar 

  • Harmar A, Keen P (1982) Synthesis, and the central and peripheral transport of substance P in dorsal root ganglion-nerve preparations in vitro. Brain Res 231:379–385

    Google Scholar 

  • Hebb C, Linzell LJ (1970) Innervation of the mammary gland. A histochemical study in the rabbit. Histochem J 2:491–505

    Google Scholar 

  • Hökfelt T, Kellerth JO, Nilsson G, Pernow B (1975) Experimental immunohistochemical studies on the localization and distribution of substance P in cat primary sensory neurons. Brain Res 100:235–252

    Google Scholar 

  • Holzer P, Bucsics A, Lembeck F (1982) Distribution of capsaicin-sensitive nerve fibers containing immunoreactive substance P in cutaneous and visceral tissues of the rat. Neurosci Lett 31:253–258

    Google Scholar 

  • Jancsó G, Király E (1981) Sensory neurotoxins: chemically induced selective destruction of primary sensory neurons. Brain Res 210:83–89

    Google Scholar 

  • Jancsó N, Jancsó-Gabor A, Szolcsányi J (1967) Direct evidence for neurogenic inflammation and its prevention by denervation and by pretreatment with capsaicin. Br J Pharmacol Chemother 31:138–151

    Google Scholar 

  • Jancsó G, Király E, Jancsó-Gabor A (1977) Pharmacologically induced selective degeneration of chemosensitive primary sensory neurones. Nature 270:741–743

    Google Scholar 

  • Jessell T, Iversen L, Cuello A (1978) Capsaicin-induced depletion of substance P from primary sensory neurons. Brain Res 152: 183–188

    Google Scholar 

  • Lembeck F (1953) Zur Frage der zentralen Übertragung afferenter Impulse III. Mitteilung. Das Vorkommen und die Bedeutung der Substanz P in den dorsalen Wurzeln des Rückenmarks. Naunyn-Schmiedeberg's Arch Exp Pathol Pharmakol 219:197–213

    Google Scholar 

  • Lembeck F, Holzer P (1979) Substance P as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation. Naunyn-Schmiedeberg's Arch Pharmacol 310: 175–183

    Google Scholar 

  • Lincoln DW, Wakerley JB (1975) Factors governing the periodic activation of supraoptic and paraventricular neurosecretory cells during suckling in the rat. J Physiol (Lond) 250:443–461

    Google Scholar 

  • Linzell JE (1950) Vasomotor nerve fibers to the mammary glands of the cat and dog. Q J Exp Physiol 35:424–429

    Google Scholar 

  • Lundberg JM, Brodin E, Hua X, Saria A (1984) Vascular permeability changes and smooth muscle contraction in relation to capsaicin-sensitive substance P afferents in the guinea-pig. Acta Physiol Scand 120:217–227

    Google Scholar 

  • Mena F, Grosvenor CE (1968) The effect of number of pups upon suckling-induced fall in pituitary prolactin concentration and milk ejection in the rat. Endocrinology 82:623–626

    Google Scholar 

  • Montagna W (1970) Histology and cytochemistry of human skin. XXXV. The nipple and areola. Br J Dermatol 83:2–13

    Google Scholar 

  • Mroz E, Brownstein M, Leeman S (1977) Distribution of immunoassayable substance P in the rat brain: evidence for the existance of substance P containing tracts. In: von Euler US and Pernow B (eds) Substance P, Raven Press, New York, pp 147–154

    Google Scholar 

  • Nagy G, Halasz B (1983) Time course of the litter removal-induced depletion of plasma prolactin levels of lactating rats. Neuroendocrinology 37:459–463

    Google Scholar 

  • Nagy JI, Hunt SP, Iversen LL, Emson PC (1981) Biochemical and anatomical observations on the degeneration of peptide-containing primary afferent neurons after neonatal capsaicin. Neuroscience 6:1923–1934

    Google Scholar 

  • Nagy J, Vincent S, Staines W, Fibiger H, Reisine T, Yamamura H (1980) Neurotoxic action of capsaicin on spinal substance P. Brain Res 186:435–444

    Google Scholar 

  • Ottesen B, Gram BR, Fahrenkrug J (1983) Neuropeptides in the female genital tract: effect on vascular smooth muscle. Peptides 4:387–392

    Google Scholar 

  • Papka RE, Furness JB, Della NG, Murphy R, Costa M (1984) The time course of effect of capsaicin on ultrastructure and histochemistry of substance P-immunoreactive nerves associated with the cardiovascular system of the guinea-pig. Neuroscience (in press)

  • Petsche U, Fleischer E, Lembeck F, Handwerker H (1983) The effect of capsaicin application to a peripheral nerve on impulse conduction in functionally identified afferent nerve fibers. Brain Res 265:233–240

    Google Scholar 

  • Theodorsson-Norheim E, Brodin E, Norheim I, Rosell S (1984) Antisera raised against eledoisin and kassinin detect immuno-reactive material in rat tissue extracts: tissue distribution and chromatographic characterization. Reg Peptides (in press)

  • Traurig H, Cotton J, Papka R, Saria A, Lembeck F (1983) Substance P in the female reproductive system and the effects of capsaicin. Neurosci Abst 9:111

    Google Scholar 

  • Traurig H, Saria A, Lembeck F (1984) Distribution of substance P in the female reproductive system. Naunyn-Schmiedeberg's Arch Pharmacol 326:343–346

    Google Scholar 

  • Vorherr H (1974) The Breast: Morphology, physiology, and lactation, Academic Press, New York, pp 34–41

    Google Scholar 

  • Wakerley JB, Lincoln DW (1971) Intermittent release of oxytocin during suckling in the rat. Nature (New Biol) 233:180–181

    Google Scholar 

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Author notes

  1. H. Traurig & R. E. Papka

    Present address: Department of Anatomy, University of Kentucky College of Medicine, 40536, Lexington, KY, USA

Authors and Affiliations

  1. Institut für experimentelle und klinische Pharmakologie der Universität Graz, Universitätsplatz 4, A-8010, Graz, Austria

    H. Traurig, R. E. Papka, A. Saria & F. Lembeck

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  1. H. Traurig
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  2. R. E. Papka
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  3. A. Saria
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  4. F. Lembeck
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Traurig, H., Papka, R.E., Saria, A. et al. Substance P immunoreactivity in the rat mammary nipple and the effects of capsaicin treatment on lactation. Naunyn-Schmiedeberg's Arch. Pharmacol. 328, 1–8 (1984). https://doi.org/10.1007/BF00496096

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  • DOI: https://doi.org/10.1007/BF00496096

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