Summary
The gold method was further developed for fluorescent microscopy. Gold granules (12 nm in size) were labelled with rhodamine conjugates of Concanavalin A and avidin. The fluorescent markers were used to mark cell wall mannan on the yeast Saccharomyces cerevisiae either by the one-step, or by the two-step method via a biotinyl derivative of ConA. By fluorescence or transmission electron microscopy, the two-step method was found to achieve a higher density of marking.
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Horisberger, M., Vonlanthen, M. Fluorescent colloidal gold: A cytochemical marker for fluorescent and electron microscopy. Histochemistry 64, 115–118 (1979). https://doi.org/10.1007/BF00493360
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DOI: https://doi.org/10.1007/BF00493360