Abstract
Tissue specificity of the esterases of two closely related species, Drosophila aldrichi and D. mulleri, is determined by starch gel electrophoresis of dissected tissues and organs and an esterase staining technique. The determination of esterase homology between the two species is supplemented by tissue specificity as an added criterion of catalytic properties. General biological functions of some of the esterases are suggested by their presence in certain tissues. The potential usefulness of knowledge of tissue specificities in evolutionary and population genetic studies is discussed.
Similar content being viewed by others
References
Beckman, L., and Johnson, F. M. (1964). Esterase variations in Drosophila melanogaster. Hereditas 51 212.
Crow, J. F. (1942). Cross fertility and isolating mechanisms in the Drosophila mulleri group. Univ. Texas Publ. 4228 53.
Doane, W. W. (1960). Developmental physiology of the mutant female sterile (2) adipose of Drosophila melanogaster. I. Adult morphology, longevity, egg production, and egg lethality. J. Exptl. Zool. 145 1.
Ferris, G. F. (1950). External morphology of the adult. In Biology of Drosophila. M. Demerec (ed.), Wiley, New York.
Johnson, F. M. (1966a). An isozyme analysis of Drosophila speciation and development. Ph.D. dissertation, University of Texas.
Johnson, F. M. (1966b). Rapid single fly homogenization for the investigation of Drosophila isozymes. Drosophila Information Service 41 193.
Johnson, F. M., and Kanapi, C. G. (1966). Esterase differences between male and female D. melanogaster. Drosophila Information Service 41 158.
Johnson, F. M., Kanapi, C. G., Richardson, R. H., Wheeler, M. R., and Stone, W. S. (1966a). An analysis of polymorphisms among isozyme loci in dark and light Drosophila ananassae strains from American and Western Samoa. Proc. Natl. Acad. Sci. (Wash.) 56 119.
Johnson, F. M., Kanapi, C. G., Richardson, R. H., Wheeler, M. R., and Stone, W. S. (1966b). An operational classification of Drosophila esterases for species comparison. Univ. Texas Publ. 6615 517.
Johnson, F. M., Richardson, R. H., and Kambysellis, M. P. (1968). Isozyme variability in species of the genus Drosophila. III. Qualitative comparison of the esterases of D. aldrichi and D. mulleri. Biochem. Genet. 1 239.
Markert, C. L., and Møller, F. (1959). Multiple forms of enzymes: Tissue, ontogenetic, and species specific patterns. Proc. Natl. Acad. Sci. (Wash.) 45 753.
Miller, A. (1950). The internal anatomy and histology of the image of Drosophila melanogaster. In Biology of Drosophila, M. Demerec (ed.), Wiley, New York.
Newby, W. W. (1942). A study of intersexes produced by a dominant mutation in Drosophila virilis, Blanco stock. Univ. Texas Publ. 4228 113.
Smithies, O. (1955). Zone electrophoresis in starch gels: Group variations in the serum proteins of normal human adults. Biochem. J. 61 629.
Wright, T. R. F. (1963). The genetics of an esterase in Drosophila melanogaster. Genetics 48 787.
Author information
Authors and Affiliations
Additional information
This work was supported (in part) by a U.S. Public Health Service research grant (GM 11609 to W. S. Stone and M. R. Wheeler) and a training grant (2T1-GM 337-07 to R. P. Wagner et al.) from the National Institutes of Health.
Rights and permissions
About this article
Cite this article
Kambysellis, M.P., Johnson, F.M. & Richardson, R.H. Isozyme variability in species of the genus Drosophila. IV. Distribution of the esterases in the body tissues of D. aldrichi and D. mulleri adults. Biochem Genet 1, 249–265 (1968). https://doi.org/10.1007/BF00485180
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00485180