Abstract
A method has been worked out that allows the detection and isolation of bacteria fermenting molecular hydrogen and carbon dioxide to acetic acid.
The ratio of methanogenic to acetogenic bacteria in sludge and lake sediment samples has been found to be approximately 100 to 1. Acetogenic bacteria could not be detected in rumen samples.
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Andreesen, J. R., Schaupp, A., Neurauter, C., Brown, A., Ljungdahl, L. G.: Fermentation of glucose, fructose, and xylose by Clostridium thermoaceticum: effect of metals on growth yield, enzymes, and synthesis of acetate from CO2. J. Bacteriol. 144, 743–751 (1973)
Balch, W. E., Schoberth, S., Tanner, R. S., Wolfe, R. S.: Acetobacterium, a new genus of hydrogen-oxidizing, carbon-dioxide-reducing, anaerobic bacteria. Int. J. Syst. Bacteriol. 27, 355–361 (1977)
Dorn, M., Andreesen, J. R., Gottschalk, G.: Fermentation of fumarate and L-malate by Clostridium formicoaceticum. J. Bacteriol. 133, 26–32 (1978)
Fischer, F., Lieske, R., Winzer, K.: Biologische Gasreaktionen. II. Mitteilung: Über die Bildung von Essigsäure bei der biologischen Umsetzung von Kohlenoxyd und Kohlensäure mit Wasserstoff zu Methan. Biochem. Z. 245, 2–12 (1932)
Hungate, R. E.: The rumen and its microbes. New York: Academic Press 1966
Hungate, R. E.: A roll tube method for cultivation of strict anaerobes. In: Methods in microbiology, vol. 3B (J. R. Norris, D. W. Ribbons, eds.), pp. 117–132. New York: Academic Press 1969
Karlsson, J. L., Volcani, B. E., Barker, H. A.: The nutritional requirements of Clostridium aceticum. J. Bacteriol 56, 781–782 (1948)
O'Brien, W. E., Ljungdahl, L. G.: Fermentation of fructose and synthesis of acetate from carbon dioxide by Clostridium formicoaceticum. J. Bacteriol. 109, 626–632 (1972)
Ohwaki, K., Hungate, R. E.: Hydrogen utilization by clostridia in sewage sludge. Appl. Environ. Microbiol. 33, 1270–1274 (1977)
Pantskhava, E. S.: Anaerobic formation of acetate from CH3OH, CH2O, HCOOH, Na2CO3 by cell suspensions of a thermophilic culture of Methanobacillus kuzneceovii. Dokl. Akad. Nauk SSR 188, 215–218 (1969)
Paynter, M. J. B., Hungate, R. E.: Characterization of Methanobacterium mobilis, sp. n., isolated from the bovine rumen. J. Bacteriol. 95, 1943–1951 (1968)
Prins, R. A., Lankhorst, A.: Synthesis of acetate from CO2 in the cecum of some rodents. FEMS Microbiol. Letters 1, 255–258 (1977)
Schoberth, S., Gottschalk, G.: Considerations on the energy metabolism of Clostridium kluyveri. Arch. Mikrobiol. 65, 318–328 (1969)
Schoberth, S. M., Balch, W. E.: A new hydrogen-oxidizing acetate-forming anaerobe. Abstr. Ann. Meeting, Amer. Soc. Microbiol. I 90, p. 131 (1975)
Schoberth, S.: Acetic acid from H2 and CO2. Formation of acetate by cell extracts of Acetobacterium woodii. Arch. Microbiol. 114, 143–148 (1977)
Wieringa, K. T.: Over het verdwijnen van waterstof en koolzuur onder anaerobe voorwarden. Antonie van Leeuwenhoek. J. Microbiol. Serol. 3, 263–273 (1936)
Wieringa, K. T.: The formation of acetic acid from carbon dioxide and hydrogen by anaerobic spore-forming bacteria. Antonie van Leeuwenhoek, J. Mikrobiol. Serol. 6, 251–262 (1940)
Wolin, E. A., Wolfe, R. S., Wolin, M. J.: Viologen dye inhibition of methane formation by Methanobacillus omelianskii. J. Bacteriol. 87, 993–998 (1964)
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Braun, M., Schoberth, S. & Gottschalk, G. Enumeration of bacteria forming acetate from H2 and CO2 in anaerobic habitats. Arch. Microbiol. 120, 201–204 (1979). https://doi.org/10.1007/BF00423066
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DOI: https://doi.org/10.1007/BF00423066