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Differentiation of pathogenic species of Candida by their recovery characteristics following ultraviolet irradiation

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Abstract

Each of seven pathogenic species of Candida exhibits a unique pattern of light and dark recovery responses to ultraviolet irradiation. C. guilliermondii, C. parapsilosis and C. pseudotropicalis photoreactivate whereas C. albicans, C. krusei, C. stellatoidea and C. tropicalis do not. Within eachof these groups, individual species are distinguishable by whether or not they express differential dark recovery during postirradiation growth at 25 C or 37 C on oxidative vs fermentative carbon sources, on inorganic vs amino acid nitrogen sources or in the presence rather than absence of ergosterol. Equivalent recovery patterns are obtained for species of Candida and the ascosporogenous species which are their corresponding perfect forms. These observations indicate strongly that the postirradation recovery is a reliable, species-specific characteristic of yeasts.

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References

  • Bak, A. L. and Stenderup, A. 1969. Deoxyribonucleic acid homology in yeasts. Genetic relatedness within the genus Candida. — J. Gen. Microbiol. 59: 21–30.

    Google Scholar 

  • Busbee, D. S. and Sarachek, A. 1969. Inactivation of Candida albicans. — Arch. Microbiol. 64: 289–314.

    Google Scholar 

  • Cox, B. and Game, J. 1974. Repair systems in Saccharomyces. — Mutation Res. 26: 257–264.

    Google Scholar 

  • Cox, B. S. and Parry, J. M. 1968. The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast. — Mutation Res. 6: 37–55.

    Google Scholar 

  • Fabre, F. and Moustacchi, E. 1973. Removal of pyrimidine dimers in cells of Schizosaccharomyces pombe mutated in different repair pathways. — Biochim. Biophys. Acta 312: 617–625.

    Google Scholar 

  • Game, J. C. and Cox, B. S. 1969. Saccharomyces cerevisiae mutant which may show cytoplasmic sensitivity to ultraviolet light. — Nature (Lond.) 223: 1067–1068.

    Google Scholar 

  • Howard-Flanders, P. 1973. DNA repair and recombination. — Brit. Med. Bull. 29: 226–235.

    Google Scholar 

  • Hurley, R. 1966. Pathogenicity of the genus Candida, p. 13–25. In H. I. Winner and R. Hurley, (eds.), Symposium on Candida infections. — E. and S. Livingstone LtD., Edinburgh.

    Google Scholar 

  • James, A. P. and Werner, M. M. 1965. The radiobiology of yeast. — Radiat. Bot. 5: 359–382.

    Google Scholar 

  • Kondo, S. 1974. Radiation genetics in microorganisms and evolutionary considerations. —Genetics 78: 149–161.

    Google Scholar 

  • Miller, G. R. and Sarachek, A. 1974. Absence of photoreactivating enzyme in Candida albicans, Candida stellatoidea and Candida tropicalis. — Infect. Immun. 10: 398–399.

    Google Scholar 

  • Murray, I. G. and Buckley, H. R. 1966. Serological study of Candida species, p. 44–49. In H. I. Winner and R. Hurley, (eds.), Symposium on Candida infections. — E. and S. Livingstone LtD., Edinburgh.

    Google Scholar 

  • Sarachek, A. 1958. The induction by ultraviolet radiation of heritable respiratory deficiency in Saccharomyces adapted and unadapted to aerobic respiration. — Cytologia 23: 143–148.

    Google Scholar 

  • Sarachek, A. and Bish, J. T. 1975. some phylogenetic implications of action spectra for photoreactivation of ultraviolet-inactivated yeasts. — Arch. Microbiol. 104: 73–75.

    Google Scholar 

  • Sarachek, A. and Higgins, N. P. 1972. Effects of ergosterol, palmitic acid and related simple lipids on the recovery of Candida albicans from ultraviolet irradiation. — Arch. Microbiol. 82: 38–54.

    Google Scholar 

  • Sarachek, A. and Ireland, R. 1970. Phylogenetic distribution in yeasts of the capacities for photoreactivation and for temperature-sensitive dark recovery following inactivation by ultraviolet radiation. — Can. J. Microbiol. 16: 1187–1198.

    Google Scholar 

  • Sarachek, A. and Ireland, R. 1971. Phylogenetic significances of the photoreactivable sectors of species of Hansenula. — Can. J. Microbiol. 17: 1217–1221.

    Google Scholar 

  • Sarachek, A. and Ireland, R. 1973. Photoreactivable sectors and the systematics of the genus Pichia. — Experientia 29: 131–133.

    Google Scholar 

  • Sarachek, A. and Pettriess, R. W. 1974. Influences of cellular susceptibility to amphotericin B and of post-irradiation growth conditions on inactivation of Candida albicans by ultraviolet radiation. — Mycopathol. Mycol. Appl. 54: 205–214.

    Google Scholar 

  • Schekman, R., Weiner, A. and Kornberg, A. 1974. Multienzyme systems of DNA replication. — Science 186: 987–993.

    Google Scholar 

  • Setlow, J. K., Boling, M. E. and Bollum, F. J. 1965. The chemical nature of photoreactivable lesions in DNA. — Proc. Natl. Acad. Sci. (U.S.) 53: 1430–1436.

    Google Scholar 

  • van Uden, N. and Buckley, H. 1970. Candida Berkhout, p. 893–1087. In J. Lodder, (ed.), The Yeasts, a taxonomic study. — North-Holland Publ. Co., Amsterdam.

    Google Scholar 

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These studies were added in part by a contract (AT11-1)-1772 with the U.S. Atomic Energy Commission.

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Sarachek, A., Brammer, J.A. Differentiation of pathogenic species of Candida by their recovery characteristics following ultraviolet irradiation. Antonie van Leeuwenhoek 42, 165–180 (1976). https://doi.org/10.1007/BF00399461

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  • DOI: https://doi.org/10.1007/BF00399461

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