Abstract
An analysis is given of the fatty acid composition of 18 yeast species, predominantly of the genus Saccharomyces; respiratory deficient mutant strains are included. The results are discussed from chemotaxonomical and physiological viewpoints, with special attention to unsaturated fatty acids and their relation to the petite mutation.
The fatty acid composition of anaerobically grown Saccharomyces cerevisiae remains restricted, as far as unsaturated fatty acids are concerned, to those added to the medium and it may thus differ considerably from the composition after aerobic growth. Depending on the acids added, the cells may contain either palmitoleic or linoleic acids as the sole unsaturated fatty acid after anaerobic growth and as the predominant unsaturated fatty acid after aerobic growth. In contrast to all other known eukaryotes, Schizosaccharomyces japonicus seems to possess an anaerobic pathway for synthesis of unsaturated fatty acids.
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References
Andreasen, A. A. and Stier, T. J. B. 1953. Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium. — J. Cell. Comp. Physiol. 41: 23–36.
Andreasen, A. A. and Stier, T. J. B. 1954. Anaerobic nutrition of Saccharomyces cerevisiae. II. Unsaturated fatty acid requirement for growth in a defined medium. — J. Cell. Comp. Physiol. 43: 271–281.
Babij, T., Moss, F. J. and Ralph, B. J. 1969. Effects of oxygen and glucose levels on lipid composition of the yeast Candida utilis grown in continuous culture. — Biotechnol. Bioeng. 11: 593–603.
Brown, C. M. and Johnson, B. 1970. Influence of the concentration of glucose and galactose on the physiology of Saccharomyces cerevisiae in continuous culture. — J. Gen. Microbiol. 64: 279–287.
Brown, C. M. and Johnson, B. 1971. Influence of oxygen tension on the physiology of Saccharomyces cerevisiae in continuous culture. — Antonie van Leeuwenhoek 37: 477–487.
Brown, C. M. and Rose, A. H. 1969. Effects of temperature on composition and cell volume of Candida utilis. — J. Bactericl. 97: 261–272.
Bulder, C. J. E. A. 1964a. Induction of petite mutation and inhibition of synthesis of respiratory enzymes in various yeasts. — Antonie van Leeuwenhoek 30: 1–9.
Bulder, C. J. E. A. 1964b. Lethality of the petite mutation in petite negative yeasts. — Antonie van Leeuwenhoek 30: 442–454.
Bulder, C. J. E. A. 1971. Anaerobic growth, ergosterol content and sensitivity to a polyene antibiotic, of the yeast Schizosaccharomyces japonicus. — Antonie van Leeuwenhoek 37: 353–358.
De Deken, R. H. 1966. The Crabtree effect and its relation to the petite mutation. — J. Gen. Microbiol. 44: 157–165.
Hoppe, W. 1960. Die Lipide der Hefen, p. 465–513. In F. Reiff et al. (ed.), Die Hefen, I Band. — Hans Carl, Nürnberg.
Johnson, B. and Brown, C. M. 1972. A possible relationship between the fatty acid composition of yeasts and the “petite” mutation. — Antonie van Leeuwenhoek 38: 137–144.
Johnson, B., Nelson, S. J. and Brown, C. M. 1972. Influence of glucose concentration on the physiology and lipid composition of some yeasts. — Antonie van Leeuwenhoek 38: 129–136.
Kates, M. 1966. Biosynthesis of lipids in microorganisms. — Annu. Rev. Microbiol. 20: 13–44.
Millbank, J. W. 1962. The action of acriflavine on yeast protoplasts. — Antonie van Leeuwenhoek 28: 215–220.
Proudlock, J. W., Haslam, J. M. and Linnane, A. W. 1969. Specific effect of unsaturated fatty acid depletion on mitochondrial oxidative phosphorylation in Saccharomyces cerevisiae. — Biochem. Biophys. Res. Commun. 37: 847–852.
Resnick, M. A. and Mortimer, R. K. 1966. Unsaturated fatty acid mutants of Saccharomyces cerevisiae. — J. Bacteriol. 92: 597–600.
Stumpf, P. K., Vijay, I. and Harwood, J. L. 1972. Biosynthesis of unsaturated fatty acids by higher plant systems, p. 57–63. In Ganguly, J. and Smellie, R. M. S. (eds.). Current trends in the biochemistry of lipids. — Academic Press, London and New York.
Wisnieski, B. J., Keith, A. D. and Resnick, M. R. 1970. Double-bond requirement in a fatty acid desaturase mutant of Saccharomyces cerevisiae. — J. Bacteriol. 101: 160–165.
Wisnieski, B. J. and Kiyomoto, R. K. 1972. Fatty acid desaturase mutants of yeast: growth requirements and electron spin resonance spin-label distribution. — J. Bacteriol. 109: 186–195.
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Bulder, C.J.E.A., Reinink, M. Unsaturated fatty acid composition of wild type and respiratory deficient yeasts after aerobic and anaerobic growth. Antonie van Leeuwenhoek 40, 445–455 (1974). https://doi.org/10.1007/BF00399357
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DOI: https://doi.org/10.1007/BF00399357