Abstract
Polyamines (spermine, spermidine, and putrescine) and nucleic acids were studied during the first cell cycle after the break of dormancy of tuber slices of Helianthus tuberosus L., cv. OB1. Immediately after the break of dormancy, a marked decrease in stored arginine and glutamine and a corresponding increase of polyamines were observed. This first synthesis of polyamines were observed. This firs synthesis of polyamines occurred very early during the G1 phase, concomitant to the synthesis of RNAs. A RNA, probably messenger-like RNA, was synthesized very actively only during the first hours of activation in the culture medium plus 2,4-dichlorophenoxyacetic acid, or in water. At the onset of the S phase, after 12h of activation, an incorporation of [3H] thymidine was also detected. A second putrescine synthesis and polyamine accumulation began during the progression of the S phase. During the progression of mitosis, there was a decrease of polyamine synthesis and accumulation.
Similar content being viewed by others
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- GA7 :
-
Gibberellin A7
- MAK:
-
methylated albumin column
References
Bagni, N.: Aliphatic amines as a growth-factor of coconut milk as stimulating cellular proliferation of Helianthus tuberosus (Jerusalem artichoke) in vitro. Experientia 22, 732–736 (1966)
Bagni, N., Calzoni, G.L., Speranza, A.: Polyamines as sole nitrogen sources for Helianthus tuberosus explants in vitro. New Phytol. 80, 317–323 (1978)
Bagni, N., Corsini, E., Serafini Fracassini, D.: Growth-factors and nucleic acids synthesis in Helianthus tuberosus. I. Reversal of actinomycin D inhibition by spermidine. Physiol. Plant. 24, 112–117 (1971)
Bagni, N., Donini, A., Serafini Fracassini, D.: Content and aggregation of ribosomes during formation, dormancy and sprouting of tubers of Helianthus tuberosus. Physiol. Plant. 27, 379–375 (1972)
Bagni, N., Serafini Fracassini, D.: The role of polyamines as growth-factors in higher plants and their mechanism of action. In: Plant Growth Substances 1973, pp. 1205–1217. Tokyo: Hirokawa Publishing Co. Inc. 1974
Bagni, N., Speranza, A.: Pathways of polyamine biosynthesis during the growth of Helianthus tuberosus parenchymatic tissue. In: Plant Growth Regulators, pp. 75–78. Kudrev, T., Ivanova, I., Karanov, E., eds. Sofia, Publ. House Bulgarian Academy of Sciences 1977
Bertossi, F., Bagni, N., Moruzzi, G., Caldarera, C.M.: Spermine as a new growth-promoting substance for Helianthus tuberosus (Jerusalem artichoke) in vitro. Experientia 21, 80–81 (1965)
Blankenship, J., Walle, T.: In vitro studies of enzymatic synthesis and metabolism of N-acctylated polyamines. In: Advances in Polyamine Research, vol. 2, pp. 97–110. Campbell, R.A., Morris, D.R., Bartos, D., Daves, G.D. Jr., Bartos, F., eds. New York: Raven Press 1978
Bonner, J., Addicott, F.: Cultivation in vitro of excised pea roots. Bot. Gaz. 99, 144–170 (1937)
Cocucci, S., Bagni, N.: Polyamine-induced activation of protein synthesis in ribosomal preparation from Helianthus tuberosus tissue. Life Sci. 7, 113–120 (1968)
Duranton, H., Maille, M.: The metabolism of proline and the synthesis of the pigments in the Jerusalem artichoke. Photochem. Photobiol. 2, 111–117 (1963)
Durst, F., Duranton, H.: Quelques aspects de l'influence de la lumiére sur le métabolisme de l'arginine dans les tissus du Topinambour cultivés in vitro. Physiol. Vég. 4, 283–298 (1966)
Eraser, R.S.S.: Studies on messenger and ribosomal RNA synthesis in plant tissue cultures induced to undergo synchronous cell division. Eur. J. Biochem. 50, 529–537 (1975)
Gerner, E.W., Russell, D.H.: The relationship between polyamine accumlation and DNA replication in synchronized chinese hamster ovary cells after heat shock. Cancer Res. 37, 482–489 (1977)
Heby, O., Gray, J.W., Lindl, P.A., Marton, L.J., Wilson, C.B.: Changes in L-ornithine decarboxylase activity during the cell cycle. Biochem. Biophys. Res. Commun. 71, 99–105 (1976)
Heby, O., Sarna, G.P., Marton, L.J., Omine, M., Perry, S., Russell, D.H.: Polyamine content of AKR leukemic cells in relation to the cell cycle. Cancer Res. 33, 2959–2964 (1978)
Jänne, J., Pösö, H., Raina, A.: Polyamines in rapid growth and cancer. Biochim. Biophys. Acta 473, 241–293 (1978)
Mascarenhas, J.P., Goralnick, R.D.: Synthesis of small molecular weight RNA in the pollen tube of Tradescantia paludosa. Biochim. Biophys. Acta 240, 56–61 (1971)
Mitchell, J.P.: DNA synthesis during the early division cycles of Jerusalem artichoke callus cultures. Ann. Bot. 31, 427–435 (1967)
Raina, A.: Studies on the determination of spermidine and spermine and their metabolism in the developing chick embryo. Acta Physiol. Scand. [Suppl.] 60, 218 (1963)
Sato, T., Watanabe, A., Imaseki, H.: RNA synthesis in the early stage of aerobic incubation of potato tuber discs. Plant Cell Physiol. 19, 609–616 (1978)
Serafini Fracassini, D., Bagni, N., Calzoni, G.L., Speranza, A.: RNA nel polline di melo. Riv. Ortoflorofrutt. Ital. 6, 669–673 (1978)
Serafini Fracassini, D., Filiti, N.: The effect of GA7 on nucleic acids during the early phases of the activation of Helianthus tuberosus tuber. G. Bot. Ital. 110, 378 (1976)
Smith, T.A.: The biosynthesis of putrescine in higher plants. Ann. N.Y. Acad. Sci. 171, 988–1001 (1970)
Speranza, A., Bagni, N.: Products of L-[14C]carbamoyl]citrulline metabolism in Helianthus tuberosus activated tissue. Z. Pflanzenphysiol. 88, 163–168 (1978)
Tabor, H., Rosenthal, S.M., Tabor, C.W.: The biosynthesis of spermidine and spermine from putrescine and methionine. J. Biol. Chem. 233, 907–914 (1958)
Williams, E., Heslop-Harrison, J., Dickinson, H.G.: The activity of the nucleolus organising region and the origin of cytoplasmic nucleolus organising region and the origin of cytoplasmic nucleoloids in meiocytes of Lilium. Protoplasma 77, 79–93 (1973)
Yeoman, M.M., Davidson, A.W.: Effect of light on cell division in developing callus cultures. Ann. Bot. 35, 1085–1100 (1971)
Yeoman, M.M., Dyer, A.F., Robertson, A.I.: Growth and differentiation of plant tissue cultures. I. Changes accompanying the growth of explants from Helianthus tuberosus tubers. Ann. Bot. 29, 265–276 (1965)
Yeoman, M.M., Evans, P.K.: Growth and differentiation of plant tissue cultures. II. Synchronous cell division in developing callus cultures. Ann. Bot. 31, 323–332 (1967)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fracassini, D.S., Bagni, N., Cionini, P.G. et al. Polyamines and nucleic acids during the first cell cycle of Helianthus tuberosus tissue after the dormancy break. Planta 148, 332–337 (1980). https://doi.org/10.1007/BF00388120
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00388120