Summary
Isolated epidermal strips of Commelina communis L. showed progressively smaller stomatal openings when incubated in abscisic acid solutions ranging in concentration from 10-8 to 10-4 M. The effects were reproducible and did not appear to be affected by the presence of auxin, gibberellic acid or kinetin. This specificity suggests that this method may prove valuable as a quick, sensitive bioassay for abscisic acid and other related compounds which might be used as antitranspirants on field crops. The fungal toxin fusicoccin, previously reported to cause increased stomatal opening on intact leaves, partially reversed the closure induced by abscisic acid.
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Allaway, W. G., Mansfield, T. A.: Experiments and observations on the after effect of wilting on stomata of Rumex sanguineus. Canad. J. Bot. 48, 513–521 (1970).
Fischer, R. A.: Stomatal opening in isolated epidermal strips of Vicia faba. I. Response to light and CO2-free air. Plant Physiol. 43, 1947–1952 (1968).
—: After effect of water stress on stomatal opening potential. II. Possible causes. J. exp. Bot. 21, 386–404 (1970).
Fujino, M.: Role of adenosinetriphosphate and adenosine-triphosphatase in stomatal movement. Sci. Bull. Fac. Educ., Nagaski Univ. 18, 1–47 (1967).
Jones, R. J., Mansfield, T. A.: Suppression of stomatal opening in leaves treated with abscisic acid. J. exp. Bot. 21, 714–719 (1970).
Majernik, O.: Responses of stomata of barley and maize to phenylmercuric acetate. Biol. Plantarum 12, 419–423 (1970).
Mansfield, T. A.: Stomatal behaviour following treatment with auxin-like substances and phenylmercuric acetate. New Phytol. 66, 325–330 (1967).
Meidner, H.: The effect of kinetin on stomatal opening and the rate of intake of carbon dioxide in mature primary leaves of barley. J. exp. Bot. 18, 556–561 (1967).
Milborrow, B. V.: The identification of (+)-abscisin II [(+)-dormin] in plants and measurements of its concentration. Planta (Berl.) 76, 93–113 (1967).
Parkinson, K. J.: The effects of silicone coatings on leaves. J. exp. Bot. 21, 566–579 (1970).
Squire, G. R., Jones, M. B.: Studies on the mechanisms of action of the antitranspirant phenylmercuric acetate, and its penetration into the mesophyll. J. exp. Bot. (in the press).
Tal, M., Imber, D., Itai, C.: Abnormal stomatal behaviour and hormonal imbalance in flacca, a wilty mutant of tomato. I. Root effect and kinetin-like activity. Plant Physiol. 46, 367–372 (1970).
———: Abnormal stomatal behaviour and hormonal imbalance in flacca, a wilty mutant of tomato. II. Auxin and abscisic acid-like activity. Plant Physiol. 46, 373–376 (1970).
Thomas, D. A.: The regulation of stomatal aperture in tobacco leaf epidermal strips. I. The effect of ions. Aust. J. Biol. Sci. 23, 961–979 (1970).
Turner, N. C., Graniti, A.: Fusicoccin: a fungal toxin that opens stomata. Nature (Lond.) 223, 1070–1071 (1970).
Waggoner, P. E., Pallas, J. E.: Saving the water spent by plants. U.S. Dept. Agric. Yearbook, 213–217 (1968).
Willmer, C. M., Mansfield, T. A.: A critical examination of the use of detached epidermis in studies of stomatal physiology. New Phytol. 68, 363–375 (1969).
——: Active cation transport and stomatal opening: a possible physiological role of sodium ions. Z. Pflanzenphysiol. 61, 398–400 (1969).
Wright, S. T. C.: An increase in the inhibitor-β content of detached wheat leaves following a period of wilting. Planta (Berl.) 86, 10–20 (1969).
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Tucker, D.J., Mansfield, T.A. A simple bioassay for detecting “antitranspirant” activity of naturally occurring compounds such as abscisic acid. Planta 98, 157–163 (1971). https://doi.org/10.1007/BF00385348
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DOI: https://doi.org/10.1007/BF00385348