Abstract
The embryonic lethal mutation in the t w5 haplotype is known to map near the H-2K region of the mouse major histocompatibility complex. Additional data obtained by classical genetic methods demonstrate that the t w5 lethal gene is effectively inseparable from H-2K. No recombinants were found between H-2K and t w5 in a sample representing over 1200 mice. On a statistical basis t w5 must be less than 250 kb from the H-2K gene. In the course of these mapping studies we obtained a set of 11 intra-H-2 recombinants. We have analyzed these and three others derived from another experiment to define their breakpoints as precisely as possible. Southern blot analysis with molecular probes to the D, S, I, and K regions of the H-2 complex defines seven recombinations between the D and S regions, two between S and I, none within the I region, and five events between I and K. The last category was studied in finer detail by developing unique copy probes to the I-K boundary region. Two of the five events occurred within probably less than 6 kb of each other: these two recombinants define the centromeric limit of the location of the t w5 gene within the H-2K region. The other three I-K recombinants occurred in at least two other nearby locations. Altogether at least three, and probably all five I-K recombinants fall within a 45 kb recombinational hot spot recently identified in Mus musculus castaneus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Artzt, K.: Gene mapping within the T/t-complex of the mouse. III. t-Lethal genes are arranged in three clusters on chromosome 17. Cell 39: 565–572, 1984
Artzt, K. and Bennett, D.: Analogies between embryonic (T/t) antigens and adult major histocompatibility (H-2) antigens. Nature 256: 545–547, 1975
Artzt, K., Shin, H.-S., Bennett, D., and Dimeo-Talento, A.: Analysis of major histocompatibility complex haplotypes of t-chromosomes reveals that the majority of diversity is generated by recombination. J. Exp. Med. 162: 93–104, 1985
Bennett, D.: The T-locus of the mouse: a review. Cell 6: 441–454, 1975
Bennett, D.: T/t locus, its role in embryogenesis and its relation to classical histocompatibility systems (a review). Prog. Allergy 29: 35–53, 1981
Chakravarti, A., Buetow, K., Antonarakis, S. E., Waber, P. G., Boehm, C. D., and Kazazian, H. H.: Nonuniform recombination within the human β-globin gene cluster. Am. J. Hum. Genet. 36: 1239–1258, 1984
Chakravarti, A., Elbein, S. C., and Permutt, M. A.: Evidence for increased recombination near the human insulin gene: implications for disease association studies. Proc. Natl. Acad. Sci. U.S.A. 83: 1045–1049, 1986
Herrmann, B., Bucan, M., Mains, P. E., Frischauf, A.-M., Silver, L. M., and Lehrach, H.: Genetic analysis of the proximal portion of the mouse t complex: evidence for a second inversion within t haplotypes. Cell 44: 469–476, 1986
Jeffreys, A. J., Wilson, V., and Thein, S. L.: Hypervariable ‘minisatel-lite’ regions in human DNA. Nature 314: 67–73, 1985
Jeffreys, A. J., Wilson, V., Kelly, R., Taylor, B. A., and Bulfield, G.: Mouse DNA ‘fingerprints’: analysis of chromosome localization and germ-line stability of hypervariable loci in recombinant inbred strains. Nucleic Acids Res. 15: 2823–2836, 1987
Klein, J.: Biology ofthe Mouse Histocompatzbility-2 Complex, Springer-Verlag, New York, Heidelberg, Berlin, 1975
Klein, J.: Natural History of the Major Histocompatibility Complex, John Wiley & Sons, New York, Chichester, Brisbane, Toronto, Singapore. 1986
Lange, K., Kunkel, L., Aldridge, J., and Latt, S.-A.: Accurate and superaccurate gene mapping. Am. J. Hum. Genet. 37: 853–867, 1985
Lebo, R. V., Chakravarti, V. A., Beutow, K. H., Cheung, M. C., Cann, H., Cordell, B., and Goodman, H.: Recombination within and between the human insulin and β-globin gene loci. Proc. Natl. Acad. Sci. U.S.A. 80: 4808–4812, 1983
Lyon, M. F.: Transmission ratio distortion in mouse t-haplotypes is due to multiple distorter genes acting on a responder locus. Cell 37: 621–628, 1984
Orkin, S. H. and Kazazian, H. H., Jr.: The mutation and polymorphism of the human β-globin gene and its surrounding DNA. Annu. Rev. Genet. 18: 131–171, 1984
Shin, H.-S., Stavnezer, J., Artzt, K., and Bennett, D.: The genetic structure and origin of t-haplotypes of mice, analyzed with H-2 cDNA probes. Cell 29: 969–976, 1982
Shin, H.-S., Flaherty, L., Artzt, K., Bennett, D., and Ravetch, J.: Inversion in the H-2 complex of t-haplotypes in mice. Nature 306: 380–383, 1983a
Shin, H.-S., McCormick, P., Artzt, K., and Bennett, D.: Cis-trans test shows a functional relationship between non-allelic lethal mutations in the T/t complex. Cell 33: 925–929, 1983b
Shin, H.-S., Bennett, D., and Artzt, K.: Gene mapping within the T/t-complex of the mouse. IV. The inverted MHC is intermingled with several t-lethal genes. Cell 39: 573–578, 1984
Shiroishi, T., Sagai, T., and Moriwaki, K.: Sexual preference of meiotic recombination within the H-2 complex. Immunogenetics 25: 258–262, 1987
Silver, L. M. and Artzt, K.: Recombination suppression of mouse thaplotypes due to chromatin mismatching. Nature 290: 68–70, 1981
Silver, L. M. and Remis, D.: Five of the nine genetically defined regions of mouse t haplotypes are involved in transmission ratio distortion. Genet. Res. Comb. 49: 51–56, 1987
Skow, L. C., Nadeau, J. N., Ahn, J.-C., Shin, H.-S., Artzt, K., and Bennett, D.: Polymorphism and linkage of the αA-crystallin gene in t-haplotypes of the mouse. Genetics 116: 107–111, 1987
Steinmetz, M., Stephan, D., and Fischer-Lindahl, K.: Gene organization and recombination hotspots in the routine major histocompatibility complex. Cell 44: 895–904, 1986
Steinmetz, M., Uematsu, Y., and Fischer-Lindahl, K.: Hotspots of homologous recombination in mammalian genomes. Trends Genet. 3: 7–10, 1987
Tosi, M., Levi-Strauss, M., Duponchel, C., and Meo, T.: Sequence heterogeneity of mutine complementary DNA clones related to the C4 and C4-Slp isoforms of the fourth complement component. Philos. Trans. R. Soc. Lond. (Biol.) 306: 389–394, 1984
Uehara, H., Abe, K., Park, C.-H., Shin, H.-S., Bennett, D., and Artzt, K.: Comparison of the molecular organization of the H-2K region of two t-haplotypes: implications for the evolution of genetic diversity in the major histocompatibility complex. EMBO J. 6: 83–90, 1987
Widera, G. and Flavell, R. A.: The I region of the C57BL/10 mouse: characterization and physical linkage to H-2K of an SB β-like class 11 pseudogene, ΨAβ3. Proc. Natl. Acad. Sci. U.S.A. 82: 5500–5504, 1985
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Artzt, K., Abe, K., Uehara, H. et al. Intra-H-2 recombination in t haplotypes shows a hot spot and close linkage of l tw5 to H-2K . Immunogenetics 28, 30–37 (1988). https://doi.org/10.1007/BF00372526
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00372526