Abstract
A portion of the X chromosome was examined for puffing activity shortly before puparium formation in several species of Hawaiian Drosophila. A large puff was induced by incubating excised salivary glands with β ecdysone. In two of the seven species studied, the region in question has been moved by inversion to a new position relative to the banding sequence in the other species, but the reaction to β ecdysone remained unchanged. Another puff, not affected by short term incubations with β ecdysone, displayed different degrees of activity in some homosequential species, affording a cytological means for distinguishing these species.
Similar content being viewed by others
Literature
Ashburner, M.: On the problem of genetic similarity between sibling species, puffing patterns in Drosophila melanogaster and D. simulans. Amer. Naturalist 103, 189–197 (1969).
Ashburner, M.: A prodromus to the genetic analysis of puffing in Drosophila. Cold Spr. Harb. Symp. quant. Biol. 35, 533–538 (1970).
Ashburner, M., Lemeunier, F.: Patterns of puffing activity in the salivary gland chromosomes of Drosophila. VII. Homology of puffing patterns on chromosome arm 3 L in D. melanogaster and D. yakuba, with notes on puffing in D. teissieri. Chromosoma (Berl.) 38, 283–295 (1972).
Berendes, H. D.: The hormone ecdysone as effector of specific changes in the pattern of gene activities of Drosophila hydei. Chromosoma (Berl.) 22, 274–293 (1967).
Carson, H. L.: Polytene chromosome relationships in Hawaiian species of Drosophila V. Additions to the chromosomal phylogeny of the picture-winged species. Studies in Genetics. VI. Univ. of Texas Publ. 7103, 183–191 (1971).
Carson, H. L., Clayton, F. E., Stalker, H. D.: Karyotype stability and speciation in Hawaiian Drosophila. Proc. nat. Acad. Sci. (Wash.) 57, 1280–1285 (1967).
Carson, H. L., Hardy, D. E., Spieth, H. T., Stone, W. S.: The evolutionary biology of the Hawaiian Drosophilidae. In: Essays in evolution and genetics in honor of Theodosius Dobzhansky (M.K. Hecht and W.C. Steere, eds.). Evol. Biol. Supplement. New York: Appleton-Century-Crofts 1970.
Crick, F. E.: General model for the chromosome of higher organisms. Nature (Lond.) 234, 25–27 (1971).
Hardy, D. E.: Insects of Hawaii, vol. 12. Honolulu: Univ. of Hawaii Press 1965.
Hardy, D. E., Kaneshiro, K.: New picture-winged Drosophila from Hawaii. Studies in Genetics. IV. Univ. of Texas Publ. 6818, 171–262 (1968).
Leenders, H. J., Wullems, G.K., Berendes, H.D.: Competitive interaction of adenosine 3–5 monophosphate in gene activation by eedysterone. Exp. Cell Res. 63, 159–164 (1970).
Raikow, R., Fristrom, J. W.: Effects of β ecdysone on RNA metabolism of imaginai discs of Drosophila melanogaster. J. Insect Physiol. 17, 1599–1614 (1971).
Wheeler, M. R., Clayton, F. E.: A new Drosophila culture technique. Dros. Inf. Serv. 40, 98 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Raikow, R.B. Puffing in salivary gland chromosomes of picture-winged Hawaiian Drosophila . Chromosoma 41, 221–230 (1973). https://doi.org/10.1007/BF00319697
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00319697