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Mise en évidence par immunofluorescence des cellules sécrétrices de glucagon dans le pancréas endocrine du poisson téléostéen Xiphophorus helleri H.

Immunofluorescent demonstration of glucagon secreting islet cells in the bony fish Xiphophorus helleri H.

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Summary

By immunofluorescence technique performed on material embedded in epoxyresin, glucagon containing islet cells were demonstrated in the teleost, Xiphophorus helleri H. Rabbit anti-pig glucagon serum was employed. Correlative tryptophan DMAB-nitrite staining shows that only some of the immunofluorescent glucagon cells are positive; this discrepancy is discussed.

Résumé

La technique d'immunofluorescence démontre l'existence de cellules sécrétrices de glucagon dans l'îlot pancréatique endocrine du Poisson téléostéen Xiphophorus helleri H. La réaction est faite sur du matériel fixé et inclus pour la microscopie électronique, avec un sérum de lapin anti-glucagon de porc. Le tryptophane est mis en évidence sur les coupes adjacentes par la réaction au DMAB-nitrite; un certain nombre de cellules fluorescentes sont négatives pour cette réaction. La différence entre les résultats donnés par les deux techniques est discutée.

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Authors and Affiliations

  1. Laboratoire de Zoologie et d'Embryologie expérimentale, Université Louis Pasteur, Strasbourg, France

    Catherine Klein

  2. Anatomisches Institut der Universität, Gießen, Deutschland

    Rainer H. Lange

Authors
  1. Catherine Klein
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  2. Rainer H. Lange
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Additional information

Travail réalisé grâce à l'aide du Deutscher Akademischer Austauschdienst et de la Deutsche Forschungsgemeinschaft.

Nous exprimons nos vifs remerciements à la firme Novo (GmbH, Mainz) qui nous a fourni le sérum anti-glucagon.

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Klein, C., Lange, R.H. Mise en évidence par immunofluorescence des cellules sécrétrices de glucagon dans le pancréas endocrine du poisson téléostéen Xiphophorus helleri H.. Histochemie 29, 213–219 (1972). https://doi.org/10.1007/BF00306145

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  • DOI: https://doi.org/10.1007/BF00306145

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