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Silver-stained structures in mammalian meiotic prophase

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Abstract

Silver staining of mammalian spermatocytes revealed, in light microscopy, synaptonemal complex and structures within the sex vesicle. It is feasible to follow the chromosome pairing phenomenon from zygotene to pachytene by examining the behavior of synaptonemal complexes. Nucleolus organizer regions take heavy silver stain in pachytene but are no longer detectable in later stages of meiosis.

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References

  • Bloom, S.E., Goodpasture, C.: An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes. Hum. Genet. 34, 199–206 (1976)

    Google Scholar 

  • Brinkley, B.R., Bryan, J.H.D.: The ultrastructure of meiotic prophase chromosomes as revealed by silver-aldehyde staining. J. Cell Biol. 32, 14 a (1964)

    Google Scholar 

  • Coleman, J.R., Moses, M.J.: DNA and the fine structure of synaptic chromosomes in the domestic rooster (Gallus domesticus). J. Cell Biol. 23, 63–78 (1964)

    Google Scholar 

  • Comings, D.E., Okada, T.A.: Whole mount electron microscopy of meiotic chromosomes and the synaptonemal complex. Chromosoma (Berl.) 30, 269–286 (1970)

    Google Scholar 

  • Counce, S.J., Meyer, G.F.: Differentiation of the synaptonemal complex and the kinetochore in Locusta spermatocytes studied by whole mount electron microscopy. Chromosoma (Berl.) 44, 231–253 (1973)

    Google Scholar 

  • Gillies, C.B.: Synaptonemal complex and chromosome structure. Ann. Rev. Genet. 9, 91–109 (1975)

    Google Scholar 

  • Goodpasture, C., Bloom, S.E.: Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma (Berl.) 53, 37–50 (1975)

    Google Scholar 

  • Hess, O., Meyer, G.F.: Chromosomal differentiations of the lampbrush type formed by the Y chromosome in Drosophila hydei and Drosophila neohydei. J. Cell Biol. 16, 527–539 (1963 a)

    Google Scholar 

  • Hess, O., Meyer, G.F.: Artspezifische funktionelle Differenzierungen des Y-heterochromatins bei Drosophila-Arten der D. hydei-Subgruppe. Portugaliae Acta Biol. (A), 7, 29–46 (1963b)

    Google Scholar 

  • Hsu, T.C., Cooper, J.E.K., Mace, Jr., M.L., Brinkley, B.R.: Arrangement of centromeres in mouse cells. Chromosoma (Berl.) 34, 73–87 (1971)

    Google Scholar 

  • Kofman-Alfaro, S., Chandley, A.C.: Meiosis in the male mouse. An autoradiographic investigation. Chromosoma (Berl.) 31, 404–420 (1970)

    Google Scholar 

  • Lau, Y.F., Pfeiffer, R.A., Arrighi, F.E., Hsu, T.C.: Combination of silver and fluorescent staining for metaphase chromosomes. Amer. J. hum. Genet. 30, 76–79 (1978)

    Google Scholar 

  • Miller, D.A., Dev, V.G., Tantravahi, R., Miller, O.J.: Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Exp. Cell Res. 101, 235–243 (1976a)

    Google Scholar 

  • Miller, O.J., Miller, D.A., Dev, V.G., Tantravahi, R., Croce, C.M.: Expression of human and suppression of mouse nucleolus activity in mouse-human somatic cell hybrids. Proc. nat. Acad. Sci. (Wash.) 73, 4531–4533 (1976b)

    Google Scholar 

  • Moses, M.J.: Chromosomal structures in crayfish spermatocytes. J. biophys. biochem. Cytol. 2, 215–218 (1956)

    Google Scholar 

  • Moses, M.J.: Synaptonemal complex karyotyping in spermatocytes of the Chines hamster (Cricetulus griseus) I. Morphology of the autosomal complement in spread preparations. Chromosoma (Berl.) 60, 99–125 (1977 a)

    Google Scholar 

  • Moses, M.J.: Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus) II. Morphology of the XY pair in spread preparations. Chromosoma (Berl.) 60, 127–137 (1977b)

    Google Scholar 

  • Moses, M.J., Slatton, G.H., Gambling, T.M., Starmer, C.F.: Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus) III. Quantitaive evaluation. Chromosoma (Berl.) 60, 345–375 (1977c)

    Google Scholar 

  • Moses, M.J., Counce, S.J.: Synaptonemal complex karyotyping in spreads of mammalian spermatocytes. In Symp. Mechanisms in Recombination, (R. Grell, ed.), pp. 385–390. New York: Plenum Press 1974

    Google Scholar 

  • Moses, M.J., Counce, S.J.: Analysis of synaptonemal complex karyotypes in five mammals. J. Cell Biol. 70, 131 a (1976)

    Google Scholar 

  • Moses, M.J., Counce, S.J., Paulson, D.F.: Synaptonemal complex complement of man in spreads of spermatocytes with details of the sex chromosome pair. Science 187, 363–365 (1975)

    Google Scholar 

  • Pathak, S., Hsu, T.C.: Chromosomes and DNA of Mus. The behavior of constitutive heterochromatin in spermatogenesis of M. dunni. Chromosoma (Berl.) 57, 227–234 (1976)

    Google Scholar 

  • Pathak, S., Hsu, T.C., Markvong, A.: Pachytene mapping of the male Chinese hamster. Cytogenet. Cell Genet. 17, 1–8 (1976)

    Google Scholar 

  • Pathak, S., Kieffer, N.M.: Sterility in hybrid cattle. I. Dustribution of constitutive heterochromatin and nucleolus organizing regions in somatic and meiotic chromosomes. Cytog. Cell genet. (in press, 1979)

  • Polani, P.E.: Centromere localization at meiosis and the position of chiasmata in the male and female mouse. Chromosoma (Berl.) 36, 343–374 (1972)

    Google Scholar 

  • Sachs, L.: Sex linkage and sex chromosomes in man. Ann. Eugen. (Lond.) 18, 255–261 (1954)

    Google Scholar 

  • Schmid, M., Hofgärtner, F.J., Zenzes, M.T., Engel, W.: Evidence for postmeiotic expression of ribosomal RNA genes during male gametogenesis. Hum. Genet. 38, 279–284 (1977)

    Google Scholar 

  • Schnedl, W.: End-to-end association of X and Y chromosomes in mouse meiosis. Nature (Lond.) New Biol. 236, 29–30 (1972)

    Google Scholar 

  • Solari, A.J.: The spatial relationship of the X and Y chromosomes during meiotic prophase in mouse spermatocytes. Chromosoma (Berl.) 29, 217–236 (1970)

    Google Scholar 

  • Tres, L.L.: Extensive pairing of the XY bivalent in mouse spermatocytes as visualized by wholemount electron microscopy. J. Cell Sci 25, 1–15 (1977)

    Google Scholar 

  • Wettstein, R., Sotelo, J.R.: Electron microscope serial reconstruction of the spermatocytes I nuclei at pachytene. J. Microscopie 6, 557–576 (1967)

    Google Scholar 

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Pathak, S., Hsu, T.C. Silver-stained structures in mammalian meiotic prophase. Chromosoma 70, 195–203 (1979). https://doi.org/10.1007/BF00288406

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