Abstract
Meiosis in the male mouse has been studied autoradiographically in air-dried preparations. Information has been obtained on the relative rates of DNA synthesis and the lengths of the S-periods in spermatogonia and spermatocytes. The average rate of synthesis in the spermatocyte is lower, and the S-period is of longer duration than the preceding spermatogonial generations. The labelling pattern of the sex-chromosomes and autosomes observed at diakinesis and metaphase II in cells labelled at the spermatocyte S-period appears to be similar to that found in cells labelled during the spermatogonial S-periods. Replication in the autosomes commences before the sex-chromosomes. Late replicating autosomal centric regions show a marked degree of asynchrony in labelling both between and within bivalents. The Y chromosome starts and finishes replication later than the X. There is a short, late-replicating, segment of the X in the vicinity of the centromere. There is a short, early-replicating segment of the Y in the vicinity of the centromere which may represent the euchromatic short arm. The X and Y appear to associate at diakinesis by the distal ends of their long arms.
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Bibliography
Callan, H. G., Taylor, J. H.: A radioautographic study of the time course of male meiosis in the newt, Triturus vulgaris. J. Cell Sci. 3, 615–626 (1968).
Carr, D. H., Walker, J. E.: Carbol fuchsin as a stain for mammalian chromosomes. Stain Teohnol. 36, 233–236 (1961).
Evans, E. P., Breckon, G., Ford, C. E.: An air-drying method for meiotic preparations from mammalian testes. Cytogenetics 3, 289–294 (1964).
Evans, H. J., Ford, C. E., Lyon, M. F., Gray, J.: DNA replication and genetic expression in female mice with morphologically distinguishable X chromosomes. Nature (Lond.) 206, 900–903 (1965).
Fawcett, D. W., Ito, S., Slautterback, D. B.: The occurrence of intracellular bridges in groups of cells exhibiting synchronous differentiation. J. biophys. biochem. Cytol. 5, 453–460 (1959).
Fraccaro, M., Hultén, M., Lindsten, J., Tiepolo, L.: A late-duplicating chromosome in spermatogonial mitosis of the mouse. Exp. Cell Res. 38, 675–677 (1965).
Galton, M., Holt, S. F.: Asynchronous replication of the mouse sex chromosomes. Exp. Cell Res. 37, 111–116 (1965).
Heslop-Harrison, J.: Cytoplasmic connections between angiosperm meiocytes. Ann. Bot., N.S. 30, 221–230 (1966).
Hotta, Y., Ito, M., Stern, H.: Synthesis of DNA during meiosis. Proc. nat. Acad. Sci. (Wash.) 56, 1184–1191 (1966).
Ito, M., Hotta, Y., Stern, H.: Studies of meiosis in vitro. II. Effect of inhibiting DNA synthesis during meiotic prophase on chromosome structure and behavior. Develop. Biol. 16, 54–77 (1967).
Lima-de-Faria, A., German, J., Ghatnekar, M., McGovern, J., Anderson, L.: In vitro labelling of human meiotic chromosomes with H3-thymidine. Hereditas (Lund) 60, 249–261 (1968).
Makino, S.: Studies on the murine chromosomes. I. Cytologioal investigations of mice included in the genus Mus. J. Fac. Sci. Hokkaido. Imp. Univ. Ser. VI Zool. 7, 305–380 (1941).
Matthey, R.: Les chromosomes des Muridae. Rev. Suisse Zool. 60, 225–283 (1953).
Meredith, R.: A simple method for preparing meiotic chromosomes from mammalian testis. Chromosoma (Berl.) 26, 254–258 (1969).
Monesi, V.: Autoradiographic study of DNA synthesis and the cell cycle in spermatogonia and spermatocytes of mouse testis using tritiated thymidine. J. Cell Biol. 14, 1–18 (1962).
Mukherjee, A. B., Cohen, M. M.: DNA synthesis during meiotic prophase in male mice. Nature (Lond.) 219, 489–490 (1968).
Mukherjee, B. B., Ghosal, S. K.: Replicative differentiation of mammalian sex chromosomes during spermatogenesis. Exp. Cell Res. 54, 101–106 (1969).
Nesbitt, M. N., Gartler, S. M.: Replication of the mouse sex chromosomes early in the S-period. Cytogenetics 9, 212–221 (1970).
Odartchenko, N., Pavillard, M.: Late DNA replication in male mouse meiotic chromosomes. Science 167, 1133–1134 (1970).
Ohno, S., Kaplan, W. D., Kinosita, R.: On the end-to-end association of the X and Y chromosomes of Mus musculus. Exp. Cell Res. 18, 282–290 (1959).
Solari, A. J.: The spatial relationship of the X and Y chromosomes during meiotic prophase in mouse spermatocytes. Chromosoma (Berl.) 29, 217–236 (1970).
Swift, H. H.: The constancy of desoxyribose nucleic acid in plant nuclei. Proc. nat. Acad. Sci. (Wash.) 36, 643–654 (1950).
Taylor, J. H., McMaster, R. D.: Autoradiographic and microphotometric studies of DNA during microgametogenesis in Lilium longiflorum. Chromosoma (Berl.) 6, 489–521 (1954).
Tiepolo, L., Fraccaro, M., Hulten, M., Lindsten, J., Mannini, A., Ming, Pen-Ming, L.: Timing of sex chromosome replication in somatic and germ-line cells of the mouse and rat. Cytogenetics 6, 51–66 (1967).
Wimber, D. E., Prensky, W.: Autoradiography with meiotic chromosomes of the male newt (Triturus viridescens) using H3-thymidine. Genetics 48, 1731–1738 (1963).
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Kofman-Alfaro, S., Chandley, A.C. Meiosis in the male mouse. An autoradiographic investigation. Chromosoma 31, 404–420 (1970). https://doi.org/10.1007/BF00285832
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DOI: https://doi.org/10.1007/BF00285832