Summary
The insulin, glucagon, somatostatin and pancreatic polypeptide content of acid-ethanol extracts of pancreas from lean and obese (ob/ob) mice of various ages was determined by radioimmunoassay. Rat and mouse pancreatic polypeptide react weakly with antibodies to avian, bovine and canine pancreatic polypeptide, but immunoassay for this peptide was possible using an antibody to the carboxyl-terminal hexapeptide of bovine pancreatic polypeptide and amino-terminal labelled bovine pancreatic polypeptide as tracer. The insulin, glucagon and pancreatic polypeptide content of pancreas from obese mice was greater than that of lean controls. The increase in insulin content uniformly involved ventral, dorsal and splenic lobes. Glucagon content was elevated primarily in the splenic lobe. Pancreatic polypeptide content was most significantly elevated in the splenic lobe where pancreatic polypeptide cells are infrequent in normal lean mice and this was accompanied by increased numbers of pancreatic polypeptide cells per islet in this lobe.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bray GA, York DA (1979) Hypothalamic and genetic obesity in experimental animals: an autonomic and endocrine hypothesis. Physiol Rev 59: 719–809
Gepts W, Christophe J, Mayer J (1960) Pancreatic islets in mice with the obese-hyperglycemic syndrome. Diabetes 9: 63–69
Hellman B, Brolin S, Hellerstrom C, Hellman K (1961) The distribution pattern of the pancreatic islet cell volume in normal and hyperglycemic mice. Acta Endocrinol. 36: 609–616
Findlay JA, Rookledge KA, Beloff-Chain A, Lever JD (1973) A combined biochemical and histological study on the islets of Langerhans in the genetically obese hyperglycaemic mouse and in the lean mouse, including observations on the effects of streptozotocin treatment. J Endocrinol 56: 571–583
Stauffacher W, Lambert AE, Vecchio D, Renold AE (1967) Measurements of insulin activities in pancreas and serum of mice with spontaneous (“obese” and “New Zealand obese”) and induced (gold thioglucose) obesity and hyperglycaemia, with considerations on the pathogenesis of the spontaneous syndrome. Diabetologia 3: 230–237
Malaisse WJ, Malaisse-Lagae F, Coleman DL (1968) Insulin secretion in experimental obesity. Metabolism 17: 802–807
Genuth SM (1969) Hyperinsulinemia in mice with genetically determined obesity. Endocrinol 84: 386–391
Patel YC, Cameron DP, Stefan Y, Malaisse-Lagae F, Orci L (1977) Somatostatin: widespread abnormality in tissues of spontaneously diabetic mice. Science 198: 930–931
Petersson B, Lundqvist G, Andersson A (1979) Somatostatin content and release from pancreatic islets from obese-hyperglycemic mice. Experentia 35: 127–128
Makino H, Matsushima Y, Kanatsuka A, Yamamoto M, Kumagai A, Nishimura M (1979) Changes in pancreatic somatostatin content in spontaneously diabetic mice, as determined by radioimmunoassay and immunohistochemical methods. Endocrinology 104: 243–247
Dolais-Kitabgi J, Le Marchand-Brustel Y, Freychet P (1979) Somatostatin in the pancreas and hypothalamus of obese mice. Diabetologia 17: 257–261
Malaisse-Lagae F, Carpentier J-L, Patel YC, Malaisse WJ, Orci L (1977) Pancreatic polypeptide: a possible role in the regulation of food intake. Experientia 33: 915–917
Baetens D, Stefan Y, Ravazzola M, Malaisse-Lagae F, Coleman DL, Orci L (1978) Alteration of islet cell populations in spontaneously diabetic mice. Diabetes 27: 1–7
Strautz RL (1970) Studies of hereditary obese mice (ob/ob) after implantation of pancreatic islets in millipore filter capsules. Diabetologia 6: 306–312
Gates RJ, Hunt MI, Smith R, Lazarus NR (1972) Return to normal of blood glucose, plasma insulin, and weight gain in New Zealand obese mice after implantation of islets of Langerhans. Lancet: 567–570
Gates RJ, Hunt MI, Lazarus NR (1974) Further studies on the amelioration of the characteristics of New Zealand obese (NZO) mice following implantation of islets of Langerhans. Diabetologia 10: 401–406
Gates RJ, Lazarus NR (1977) The ability of pancreatic polypeptide (APP and BPP) to return to normal the hyperglycemia, hyperinsulinemia and weight gain of New Zealand obese mice. Hormone Res 8: 189–202
Gingerich RL, Gersell DJ, Greider MH, Finke EH, Lacy PE (1978) Elevated levels of pancreatic polypeptide in obese hyperglycemic mice. Metabolism 27: 1526–1532
Mordes JP, Eastwood GL, Loo S, Rossini AA (1982) Pancreatic polypeptide causes diarrhea and weight loss in obese mice but not in lean littermates. Peptides 3: 873–875
Marco J, Zulueta MA, Correas I, Villaneuva ML (1980) Reduced pancreatic polypeptide secretion in obese subjects. Endocrinology 50: 744–747
Lassmann V, Vague P, Vialettes B, Simon MC (1980) Low plasma levels of pancreatic polypeptide in obesity. Diabetes 29: 428–430
Ziff G, O'Dorisio M, Cataland S, Dixon K (1983) Pancreatic polypeptide responses to protein meal challenges in obese but otherwise normal children and obese children with Prader-Willi syndrome. J Clin Endocrinol Metab 57: 1074–1080
Kimmel JR, Hayden LJ, Pollock HG (1975) Isolation and characterization of a new pancreatic polypeptide hormone. J Biol Chem 250: 9369–9376
Baetens D, Malaisse-Lagae F, Perrelet A, Orci L (1979) Endocrine pancreas: three-dimensional reconstruction shows two types of islets of Langerhans. Science 206: 1323–1325
Morgan CR, Lazarow A (1963) Immunoassay of insulin: two antibody system. Diabetes 12: 115–126
Tomita T (1980) Effect of alloxan on insulin and glucagon secretion in perifused islets. Diabetologia 19: 154–157
Lindall AW (1979) Somatostatin by radioimmunoassay. Immunonuclear Corporation Publications, Stillwater, MI, pp 20–21
Pollock HG, Kimmel JR (1981) Immunoassay for avian pancreatic polypeptide and applications in chickens. Gen Comp Endocrinol 45: 386–394
Bolton AE, Hunter WM (1973) The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J 133: 529–538
Arimura A, Sato H, Dupont A, Nishi N, Schally AV (1981) Somatostatin: abundance of immunoreactive hormone in rat stomach and pancreas. Science 189: 1007–1009
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tomita, T., Doull, V., Kimmel, J.R. et al. Pancreatic polypeptide and other hormones in pancreas of obese (ob/ob) mice. Diabetologia 27, 454–459 (1984). https://doi.org/10.1007/BF00273910
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00273910