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On the ability of Salmonella typhimurium cells to form deoxycytidine nucleotides

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Summary

It is known that cdd − S. typhimurium mutants selected for resistance to 5-fluorodeoxycytidine (FdCyd) possess no deoxycytidine kinase activity. The present study postulates that this method of screening selects double mutants defective in cytidine deaminase and in deoxycytidine kinase. To prove this hypothesis, the cdd mutant of S. typhimurium was constructed by P1-mediated transfer of ccd - gene into a new genetic background, and the activity of deoxycytidine kinase was assayed. Transductants exhibited no deoxycytidine kinase activity, showing that the absence of this enzyme is not limited to a specific cdd - mutant, but includes all strains of S. typhimurium. The toxicity of FdCyd for the bacterial strains possessing deoxycytidine kinase, as well as the role of nucleoside phosphorylase in nucleotide formation by S. typhimurium, is discussed.

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References

  • Antón, D.N.: Histidine regulatory mutants in Salmonella typhimurium V. Two new classes of histidine regulatory mutants. J. molec. Biol. 33, 533–546 (1968)

    Google Scholar 

  • Beck, C.F., Ingraham, J.L.: Location on the chromosome of Salmonella typhimurium of genes governing pyrimidine metabolism. Molec. gen. Genet. 111, 303–316 (1971)

    Google Scholar 

  • Brawerman, G., Chargaff, E.: On the synthesis of nucleotides by nucleoside phosphotransferases. Biochim. biophys. Acta (Amst.) 15, 549–559 (1954)

    Google Scholar 

  • Brunngraber, E.F., Chargaff, E.: A nucleotide phosphotransferase from Escherichia coli. Biochem. 12, 3005–3012 (1973)

    Google Scholar 

  • Cooper, G.M., Greer, S.: Phosphorylation of 5-halogenated deoxycytidine analogues by deoxycytidine kinase. Molec. Pharmacol. 9, 704–710 (1973a)

    Google Scholar 

  • Cooper, G.M., Greer, S.: The effect of inhibition of cytidine deaminase by tetrahydrouridine on the utilization of deoxycytidine and 5-bromodeoxycytidine for deoxyribonucleic acid synthesis. Molec. Pharmacol. 9, 698–703 (1973b)

    Google Scholar 

  • Hanze, A.R.: Nucleic acids. IV. The catalytic reduction of pyrimidine nucleosides. J. Amer. chem. Soc. 89, 6720–6725 (1967)

    Google Scholar 

  • Kallings, L.O.: Sensitivity of various Salmonella strains to Felix 0–1 phage. Acta path. microbiol. scand. 70, 446–454 (1967)

    Google Scholar 

  • Karlström, H.O., Inability of Escherichia coli B to incorporate added deoxycytidine, deoxyadenosine and deoxyguanosine into DNA. Europ. J. Biochem. 17, 68–71 (1970)

    Google Scholar 

  • Katagiri, H., Yamada, H., Mitsugi, K., Tsunoda, T.: Bacterial synthesis of nucleotides Part I. Nucleoside phosphotransferase of Escherichia coli. Agr. Biol. Chem. 28, 577–585 (1964)

    Google Scholar 

  • Miller, J.H.: Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory, 1972

    Google Scholar 

  • Mitsugi, K., Komagata, K., Takahashi, M., Itzuka, H., Katagiri, H.: Bacterial synthesis of nucleotides Part II. Distribution of nucleoside phosphotransferases in bacteria. Agr. Biol. Chem. 28, 586–600 (1964)

    CAS  PubMed  Google Scholar 

  • Mojica-A, T.: Transduction by phage P1CM clr-100 in Salmonella typhimurium. Molec. gen. Genet. 138, 113–126 (1975)

    Google Scholar 

  • Neuhard, J.: Pyrimidine nucleotide metabolim and pathways of thymidine triphosphatase biosynthesis in Salmonella typhimurium. J. Bact. 96, 1519–1527 (1968)

    Google Scholar 

  • Neuhard, J., Ingraham, J.: Mutants of Salmonella typhimurium requiring cytidine for growth. J. Bact. 95, 2431–2433 (1968)

    Google Scholar 

  • Popowska, E., Janion, C.: The metabolism of N4-hydroxycytidine—a mutagen for Salmonella typhimurium. Nucleic Acids Res. 2, 1143–1151 (1975)

    Google Scholar 

  • Rosner, J.L.: Formation induction, and curing of bacteriophage Pl lysogens. Virology 48, 679–689 (1972)

    Google Scholar 

  • Roth, J.R.: UGA nonsence mutation in Salmonella typhimurium. J. Bact. 102, 467–475 (1970)

    Google Scholar 

  • Shatkin, A.J., in: Fundamental techniques in virology, pp. 231–237, eds. K. Habel and N.P. Salzman. New-York-London: Academic Press 1969

    Google Scholar 

  • Taylor, A.L., Trotter, C.D.: Revised linkage map of Escherichia coli. Bact. Rev. 31, 332–353 (1967)

    Google Scholar 

  • Vogel, H.J., Bonner, D.M.: Acetylornitase of Escherichia coli: partial purification and some properties. J. biol. Chem. 218, 97–106 (1956)

    Google Scholar 

  • Washmann, J.T., Morgan, D.D.: Demonstration of a deoxycytidine kinase activity in extracts of Bacillus meganterium K M. Appl. Microbiol. 25, 506–508 (1973)

    Google Scholar 

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  1. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Rakowiecka 36, 02-532, Warszawa, Poland

    Celina Janion

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  1. Celina Janion
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Communicated by F. Kaudewitz

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Janion, C. On the ability of Salmonella typhimurium cells to form deoxycytidine nucleotides. Molec. Gen. Genet. 153, 179–183 (1977). https://doi.org/10.1007/BF00264733

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  • DOI: https://doi.org/10.1007/BF00264733

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